博碩士論文 88326009 詳細資訊




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姓名 陳睿斌(JUI-PIN CHEN )  查詢紙本館藏   畢業系所 環境工程研究所
論文名稱 應用分子生物技術進行生物處理程序菌相分析之研究
(Using Molecular Biotechnology To Investigate The Microbial Diversity Of Biological Wastewater Treatment Processes)
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摘要(中) 生物處理程序主要是利用反應槽中各不同微生物的代謝特性,以分解、去除污水中的有機物質及營養鹽類,故微生物菌種與處理效果息息相關。而由於傳統分析方法有許多缺點,故本文將利用以16S rDNA為基礎的分子生物技術,探討在實廠、A2O模廠及TNCU-I模廠的處理程序中,微生物菌群的結構、菌種的變化及其所佔比例等等,藉以對生物處理程序有更進一步的了解。
由實驗結果顯示,在各樣本的菌群結構方面,皆以相似於Proteobacteria的菌株所佔比例最大,最少的A2O為47.4 %,最多的TNCU-I活性污泥則有63.3 %。另外,在Proteobacteria的各分枝中,屬於alpha-subclass的菌株在四個樣本中均未出現,而屬於Beta-subclass的菌株,在營養鹽處理程序中為主要菌群,但在民生廠實廠中則明顯減少。Gamma-subclass在四個樣本中所在比例差異不大,均在14.6 %?21.6 %之間。
而文獻中較少提及的Delta-subclass則僅在TNCU-I活性污泥中有發現,至於Epsilon-subclass在營養鹽處理程序中出現的比例並不高,但在民生廠活性污泥中卻多達23.4 %。至於CFB group,在民生廠則有21.5 %。
在菌種比較方面,除了在民生廠活性污泥外,其餘三個樣本皆可以發現Nitrospira group的存在,可見Nitrospira group確實較易在營養鹽處理程序中生長,另外在RBC生物膜上尚發現Nitrosomonas屬的存在,故推論RBC確實提供了硝化菌較多的生長空間。
在水質實驗的結果中顯示,A2O模廠除氮效率為63.2 %,TNCU-I則
為82.9 %,兩模廠的除磷效率更是高達100 %,而由菌相分析的結果亦顯示,在A2O污泥與TNCU-I污泥中,相關於除氮及除磷的真細菌皆佔有相當的比例。
另外,在TNCU-I活性污泥中,菌相分析的結果顯示有脫硝除磷菌的存在,且比例高達13.2 %,再配合水質分析的數據,可以發現,流經厭氧槽的PO4,有47 %會在缺氧槽中被攝取,證實了在TNCU-I的污泥中,確實存在著脫硝除磷菌。
摘要(英) The microorganisms are very important in the biological nutrient removal (BNR) process due to the directly degradation of nutrient from wastewater. Different microorganism has its own metabolism, i.e., to degrade different substrate. Thus, to understand the microbial diversity of a BNR process is necessary. Since the traditional microorganism analytical method has lots of bias, this dissertation utilizes the 16S rDNA molecular biotechnology to investigate the microbial diversity of several wastewater treatment processes, include a municipal wastewater treatment plant, an A2O pilot plant and a TNCU-I pilot plant.
The result showed that the Proteobacteria was the predominant bacteria in all samples, that is 47.4%, and 63.3% in A2O and TNCU-I process, respectively. The beta-subclass of Proteobacteria was the most predominant one in A2O and TNCU-I processes, but not in municipal wastewater treatment plant. The Gamma-subclass was predominant in all samples in a range of 14.6% to 21.6 %. No alpha-subclass was identified in all samples. The Delta-subclass was only observed in TNCU-I activated sludge. The epsilon-subclass was minor in the BNR process but was predominant in municipal wastewater treatment plant that is about 23.4 %. Furthermore, all the BNR samples could observe the genus Nitrospira and genus Nitrosospira. The genus Nitrosomonas was also observed in RBC biofilm.
As the nitrogen removal performance of A2O and TNCU-I was 63.2% and 82.9 % respectively, a certain bacteria were identified as nitrogen removal bacteria in both two processes. Additionally, lots of bacteria were identified as phosphate removal bacteria, corresponding to the phosphate removal efficiency of 100% in both processes.The result showed that the nitrifier, denitrifier and phosphate removal bacteria could be observed in TNCU-I process, corresponding to the excellent nitrogen and phosphate removal efficiencies.
Among the phosphate removal bacteria in TNCU-I process, 13.2% of total bacteria were identified as DNPAO-like bacteria, which could uptake phosphate under anoxic condition by using nitrate as electron acceptor.
關鍵字(中) ★ 分子生物技術
★  生物處理
★  聚合脢連鎖反應
★  變性梯度凝膠電泳
關鍵字(英) ★ 16S rDNA
★  BNR
★  molecular biotechnology
論文目次 目錄
摘要Ⅰ
英文摘要Ⅲ
目錄Ⅳ
圖目錄Ⅵ
表目錄Ⅷ
第一章 前言1
1.1研究緣起1
1.2研究目的與內容2
第二章 文獻回顧4
2.1生物處理程序之基本理論4
2.1.1生物除碳4
2.1.2生物除氮5
2.1.3生物除磷8
2.2各處理程序之基本原理12
2.2.1 標準活性污泥法程序12
2.2.2 A2O程序12
2.2.3 TNCU-I 程序14
2.2.4 生物處理程序中常見的微生物15
2.3 以16S rDNA為基礎的分子生物技術16
2.3.1 採樣18
2.3.2 污泥總DNA的萃取19
2.3.3 PCR反應19
2.3.4 cloning21
2.3.5 族群指紋譜的建立23
2.3.6 定序及親緣樹的建立26
第三章 實驗設備與方法27
3.1 實驗規劃27
3.2 污泥來源27
3.2.1 實廠活性污泥(台北民生污水廠)27
3.2.2 A2O模廠污泥28
3.2.3 TNCU-I 模廠污泥30
3.3 實驗方法32
3.3.1 DNA萃取33
3.3.2 PCR34
3.3.3 Cloning35
3.3.4 DGGE39
3.3.5 定序及類緣樹製作40
3.4 實驗設備41
第四章 結果與討論42
4.1 實廠活性污泥之菌相分析42
4.2 模廠生物營養鹽去除程序之菌相分析49
4.2.1 A2O污泥菌相分析結果49
4.2.2 TNCU-I 之活性污泥菌相分析結果58
4.2.3 TNCU-I 之RBC菌相分析結果65
4.3 各不同污泥菌相之綜合比較71
4.3.1 生物營養鹽程序活性污泥之菌相綜合比較71
4.3.2 標準活性污泥與生物營養鹽去除程序菌相之綜合比較78
4.3.3 TNCU-I之活性污泥與RBC污泥菌相之綜合比較82
4.4 綜合討論87
第五章 結論與建議90
5.1結論90
5.2 建議92
參考文獻93
參考文獻 1.中華民國環境工程學會,(2000) 環境微生物
2.經濟部工業局,(1995) 廢水處理功能生物診斷技術
3.歐陽嶠暉,(2000)下水道工程學,長松出版社
4.蘇昭郎,(1996) 厭氧好氧RBC及活性污泥法去除營養鹽之特性研究,國立
中央大學環境工程研究所博士論文
英文參考資料:
6.Amann R.I., Binder B., Chisholm S.W., Olsen R., Devereux R.
and Stahl D.A. (1990) Combination of 16S rRNA-targeted
oligonucleotide probes with flow cytometry for analyzing
mixed microbial populations. Appl. Environ. Microbiol. 56(6),
1919-1925
7.Amann R.I., Stromley J., Devereux R., Key R., and Stahl D.A.
(1992) Molecular and microscopic identification of sulfate-
reducing bacteria in multispecies biofilms. Appl. Environ.
Microbiol. 58(2), 614-623
8.Amann R.I., Ludwig W. and Schleifer K.-H.(1995) Phylogenetic
identification and in situ detection of individual microbial
cells without cultivation. Microbiological Reviews 59(1),143-
169
9.Blackall L.L.(1994) Molecular identification of activated
sludge foaming bacteria. Wat. Sci. Technol. 29(7),35-42
10.Blackall L.L, Burrell P.C., Gwilliam H., Bradford D., Bond
P.L.and Hugenholtz P.(1998) The use of 16S rDNA clone
libraries to describe the microbial diversity of activated
sludge communities. Wat. Sci. Technol. 37(4-5),451-454
11.Bodrossy L., Holmes EM., Holmes AJ., Kovacs KL. and Murrell
JC.(1997) Analysis of 16S rRNA and methane monooxygenase
gene sequences reveals a novel group of thermotolernt and
thermophilic methanotrophs, Methylocaldum gen.nov. Arch.
Microbiol. 168(6),493-503
12.Bond P.L., Hugenholtz P., Keller J. and Blackall L.L.
(1995) Bacterial community structures of phosphate-removing
and non-phosphate removing activated sludges from sequencing
batch reactors. Applied and Environmental Microbiology. 61
(5),1910-1916
13.Brodisch K.E.U. and Joyner S.J.(1983) The role of
microorganisms other than Acinetobacter in biological
phosphate removal in activated sludge process. Wat. Sci.
Technol .15(3-4) ,117-125
14.Brodisch K.E.U. Interaction of different groups of
microorganisms in biological phosphate removal. Water
Sci.Technol. 17(12),89-97
Burrell P.C., Keller J. and Blackall L.L.(1998) Microbiology
of a nitrite-oxidizing bioreactor. Appl. and Environ.
Microbiology 64(5), 1878-1883
15.Chuang S.H., Ouyang C.F., Yuang H.C. and You S.J., (1997)
Phosphorus and polyhydroxyalkanoates variation in a combined
process with activated sludge and bilfilm. Wat. Sci.
Technol. 37(4-5), 593-597
16.Comeau Y., Oldham W.K. and Hall K.J. (1987) Dynamics of
carbon reserves in biological dephosphatation of wastewater.
In: Biological Phosphate Removal from Wastewater ,R.Ramadori
(ed.),Pergamon Press Oxford, 39-55
17.Comeau Y., Hall K.J. and Oldham W.K.(1988) Determination of
poly-β-hydroxyvalerate in activated sludge by gas-liquid
chromatography. Appl. Environ.Microbiol. 54 (9) , 2325-2327
18.Crocetti G.R., Hugenholtz P., Bond P.L., Schuler A., Keller
J., Jenkins D. and Blackall L.L. (2000) Identification of
polyphosphate-accumulating organisms and design of 16S
rRNA directed probes for their detection and quantitation.
Applied and Environmental Microbiology. 66(3), 1175-1182
19.Cummings DE., Caccavo F., Spring S. and Rosenzweig R.F.
(1999) Ferribacterium limneticum gen.nov., sp.nov.,an Fe(3+)-
reducing microorganism isolated from mining-impacted
freshwater lake sediments. Arch. Microbiol. 171(3) ,183-188
20.Doronina NV. and Trotsenko YA.(1994) Methylophilus
leisingerii sp. nov.,a new species of restricted
facultatively methylotrophic bacteria. Microbiology. 63(3) ,
298-302
21.Felske A., Engelen B., Nubel U. and Backhaus H. (1996)
Direct ribosomal isolation from soil to extract bacterial
rRNA for community analysis. Appl. Environ. Microbiol.62
(11) ,4162-4167
22.Ferris M.J., Muyzer G. and Ward D.W. (1996) Denaturing
gradient gel electrophoresis profiles of 16S rRNA-defined
populations inhabiting a hot spring microbial mat community.
Appl. Environ.Microbiol.62(2), 340-346
23.Ferris M. J. and Ward D. M. (1997) Seasonal Distributions of
Dominant 16S rRNA-Defined Populations in a hot spring
microbial mat examined by denaturing gradient gel
electrophoresis. Appl. Environ. Microbiol. 63(4), 1375-1381
24.Fukui M., Teske A., Assmus B., Muyzer G. and Widdel F.
(1999) Physiology, phylogenetic relationships, and ecology
of filamentous sulfate-reducing bacteria(genus desulfonema).
Arch Microbiol. 172(4) ,193-203
25.Hallbeck L, Stahl F, Pedersen K.(1993) Phylogeny and
phenotypic characterization of stalk-forming and iron-
oxidizing bacterium Gallionella ferruginea. J Gen
Microbiol.139(7),1531-1535
26.Hallin S. and Lindgren P.-E. (1999) PCR detection of genes
encoding nitrite reductase in denitrifying bacteria. Applied
and Environmental Microbiology. 65(4) , 1652-1657
27.Hesselmann RP., Werlen C., Hahn D., van der Meer JR. and
Zehnder AJ.(1999) Enrichment, phylogenetic analysis and
detection of a bacterium that performs enhanced biological
phosphate removal in activated sludge. Syst. Appl.
Microbiol. 22(3), 454-465
28.Hiorns WD., Hastings RC., Head IM., McCarthy AJ., Saunders
JR., Pickup RW. and Hall GH. (1995) Amplification of 16S
ribosomal RNA genes of autotrophic ammonia-oxidizing
bacteria demonstrates the ubiquity of nitrosospira in the
environment. Microbiology 141(11), 2793-2800
29.Hippe H., Hagelstein A., Kramer I., Swiderski J.and
Stackebrandt E. (1999) Phylogenetic analysis of Formivibrio
citricus propionivibrio dicarboxylicus, anaerobiospirillum
thomasii, succinimonas amyloytica and succinivibrio
dextrinosolvens and proposal of succinivibrionaceae fam.nov.
Int.J. Syst.Bacteriol. 49(2), 779-782
30.Hiraishi A.(1988) Respiratory quinone profiles as tools for
identifying different bacterial populations in activated
sludge. J. Gen. Appl. Microbiol. 34(1), 39-56
31.Hiraishi A., Masamune K. and Kitamura.H.(1989)
Characterization of the bacterial population structure in an
anaerobic-aerobic activated sludge system on the basis of
respiratory quinone profiles. Appl. Environ. Microbiol.55
(4), 897-901
32.Hiraishi A. and Morishima Y.(1990) Capacity for
polyphosphate accumulation of predominant bacteria in
activated sludge showing enhanced phosphate removal. J.
Ferment. Bioeng.69 (6) , 368-371
33.Hurlbert RE., Weckesser J., Tharanathan RN.and Mayer H.(1978)
Isolation and character of the lipopolysaccharide of
Thiocapsa roseopersicina., Eur. J. Biochem. 90(2), 241-246
34.Holt G.J., Krieg R.N., Sneath H.P., Staley T.J. and Williams
T.S.(1994) Bergey’s mannal of determinatire
bacteriology.9th Edition ,Williams and Wilkins,Maryland.
Ingraham J.L., Maaloe O. and Neidhardt F.C.(1983) Growth of
the bacteria cell. Sunderland, Massachusetts. Sinauer,12-107
35.Jenkins D.(1992) Handout for the workshop of operation and
control in activated sludge systems. Graduate Institute of
Environment Engineering, National Central University
36.Juretschko S., Timmermann G., Schmid M., Schleifer KH.,
Pommerening-Roser A., Koops HP. and Wagner M.(1998)
Combined molecular and conventional analysis of nitrifying
bacterium diversity in activated sludge: Nitrosococcus
mobilis and Nitrospira-like bacteria as dominant
populations. Appl. Environ . Microbiol.64 (8),3042-3051
37.Kowalchuk G.A., Stephen J.R., de Boer W., Prosser J. I.,
Embley T. M., and Woldendrop J.W. (1997) Analysis of
ammonia-oxidizing bacteria of the β-subdivision of the lass
proteobacteria in coastal sand dunes by denaturing gradient
gel electrophoresis and sequencing of PCR-amplified 16S
ribosomal DNA fragments. Appl. Environ. Microbiol. 63(4),
1489-1497
38.Liu W. T., Marsh T. L., Cheng H. and Forney L. J. (1997)
Characterization of microbial diversity by determining
terminal restriction fragment length polymorphisms of genes
encoding 16S rRNA. Appl. Environ. Microbiol. 63(11), 4516-
4522
39.Liu W. T., Marsh T. L. and Forney L. J.(1997) Determination
of the microbial diversity of anaerobic-aerobic activated
sludge by a novel molecular biological techniqe. Wat.
Sci.Technol. 37(3), 417-422
40.Madigan MT, Jung DO, Woese CR, Achenbach LA.(2000)
Rhodoferax antarcticus sp.nov., a moderately psychrophilic
purple nonsulfur bacterium isolated from an Antarctic
microbial mat. Arch. Microbiol. 173(4), 269-277
41.Madigan T.M., Martinko J.M. and Parker J.(2000) Brock
biology of microorganisms 9th: Prokaryotic diversity:
bacteria,Sourthern Illinois University Carbondale. Prentice
Hall Tnternational Inc, 453-544
42.Manfredi R., Nanetti A., Ferri M., Mastroianni A., Coronado
O.V. and Chiodo F. (1999) Flavobacterium spp. organisms as
opportunistic bacterial pathogens during advanced HIV
disease.J Infect 39(2),146-152
43.Manz W., Wagner M., Amann R. and Schleifer K.H. (1994) In
situ characterization of the microbial consortia active in
two wastewater treatment plants., Water Res.28 (8) ,1715-1723
44.McCammon SA., Innes BH., Bowman JP., Franzmann PD., Dobson
SJ., Holloway PE., Skerratt JH., Nichols PD. and Rankin LM.
(1998) Flavobacterium hibernum sp. nov., a lactose-utilizing
bacterium from a freshwater Antarctic lake. Int.J.
Syst.Bacteriol. 48 (4), 1405-1412
45.Mino T. (2000) Microbial selection of polyphosphate-
accumulating bacteria in activated sludge wastewater
treatment processes for enhanced biological phosphate
removal. Biochemistry 65(3),341-348
46.Muyzer G., Dewaal E.C. and Uitterlinden A.G. (1993)
Profiling of complex microbial populations by denaturing
gradient gel electrophoresis analysis of polymerase chain
reaction amplified genes coding for 16S rRNA. Appl. Environ.
Microbiol.59 (3) , 695-700
47.Muyzer G. and Smalla K. (1998) Application of denaturing
gradient gel electrophoresis (DGGE) and temperature gradient
gel electrophoresis (TGGE) in microbial ecology. Antonie van
Leeuwenhoek. 73 (1) , 127-141
48.Myers R.M., Fisher S.G., Lerman L.S. and Maniatis T.(1985)
Nearly all single base substitutions in DNA fragments joined
to a GC-clamp can be detected by denaturing gradient gel
electrophoresis.,Nucleis Acids Res. 13(9) ,3131-3145
49.Myers R.M., Maniatis T. and Lerman L.S. (1987) Detection and
localization of single base changes by denaturing gradient
gel electrophoresis. Methods in Enzymology 155, 501-527
50.Nielsen PH., de Muro MA., Nielsen JL.(2000) Studies on the
in situ physiology of Thiothrix spp. Present in activated
sludge., Environ Microbiol. 2 (4), 389-398
51.Pace N.R. (1996) New perspectives on the natural microbial
world : molecular microbial ecology. ASM news 62,463-470
52.Randall C.W., Barnard J.L. and Stensel H.D. (1992) Design
and Retrofit of Wastewater Treatment Plants for Biological
Nutrient Removal. Chapters1-4 , Technomic Publishing Company
Inc.,Pennsylvania
53.Saiki R. K., Gelfand D. H., Stoffel S. J., Higuchi R., Horn
G. T., Mullis K. B. and Erlich H. A.(1988) Primer-directed
enzymatic amplification of DNA with thermostable DNA
polymerase. Science 239 (4839), 487-491
54.Santegoeds C.M., Nold S.C. and de Beer D.(1996) Denaturing
gradient gel electrophoresis used to monitor the enrichment
culture of aerobic chemoorganotrophic bacteria from a hot
spring cyanobacterial mat. Appl. Environ. Microbiol. 62
(11), 3922-3928
55.Scheffield V. C., Beck J. S., Stone E. M. and Myers R. M. A
(1992) Simple and efficient method for attachment of a 40-
base pair, GC-rich sequence to PCR amplified DNA.
BioTechniques 12(3), 386-387
56.Schulze R., Spring S., Amann R., Huber I., Ludwig W.,
Schleifer KH., Kampfer P. (1999) Genotypic diversity of
Acidovorax strains isolated from activated sludge and
description of Acidovorax defluvii sp. nov. Syst Appl
Microbol .22(2), 205-214
57.Schweitzer B., Huber I.I., Amann R., Ludwig W. and Simon M.
(2001) alpha- and beta-Proteobacteria control the
consumption and release of amino acids on lake snow
aggregates. Appl. Environ. Microbiol. 67(2), 632-645
58.Snaidr J., Amann R., Huber I., Ludwig W., Schleifer K.H.
(1997) Phylogenetic analysis and in situ identification of
bacteria in actived sludge. Applied and Environmental
Microbiology. 63(7),2884-2896
59.Snaidr J., Fuchs B., Wallner G., Wagner M., Schleifer KH.
and Amann R.(1999) Phylogeny and in situ identification of a
morphologically conspicuous bacterium, Candidatus Magnospira
bakii, present at very low frequency in activated sludge.
Environ. Microbiol. 1(2), 125-135
60.Sridhar J., Eiteman MA., Wiege JW. (2000) Elucidation of
enzymes in fermentation pathways used by Clostridium
thermosuccinogenes growing on inulin. Appl. Environ.
Microbiol. 66(1), 246-251
61.Stampi S., Varoli O., Zanetti F. and De Luca G.(1993)
Arcobacter cryaerophilus and thermophilic campylobacters in
a sewage treatment plant in Italy: two secondary treatments
compared. Epidemiol Infect.110 (3), 633-639
62.Stensel H.D. and Sedlak R.I.(1991) Principles of biological
phosphorus removal,In:Phosphorus and nitrogen removal from
municipal wastewater-principles and practices Lewis
publishers,New York.141-163
63.Stephen J.R., McCaig A.E., Smith Z., Prosser J.I. and Embely
T.M. (1996) Molecular diversity of soil and marine 16S rRNA
gene sequences related to β-subgroup ammonia-oxidizing
bacteria. Appl. Environ. Microbiol. 62 (11), 4147-4154
64.Su J.L. and Ouyang C.F.(1996) Nutrient removal using a
combined process with activated sludge and fixed biofilm.
Wat. Sci.Technol.34(1-2),477-486
65.Takeuchi J. (1991) Influence of nitrate on the bacterial
flora of activated sludge under anoxic condition .Wat. Sci.
Technol. 23(4-6),765-772
66.Vandamme P., Falsen E., Rossas R., Hoste B., Segers P.,
Tytgat R., De LeyJ. (1991) Revision of Campylobacter,
Helicobacter, and Wolinella taxonomy: emendation of generic
descriptions and proposal of Arcobacter gen.nov.. Int.J.
Syst.Bacteriol.41(1) , 88-103
67.Varma AK., Rigsby W. and Jordan DC.(1983) A new inorganic
pyrophosphate utilizing bacterium from a stagnant lake. Can
J Microbiol. 29(10),1470-1474
68.Wagner M., Amann R., Lemmer H. and Schleifer K.-H.(1993)
Probing activated sludge with proteobacteria-specific
oliginucleotides: inadequacy of culture-dependent methods
for describing microbial community structure. Appl. Environ.
Microbiol. 59(5), 1520-1525
69.Wagner M., Amann R., Kampfer P., Assmus B., Hartmann A.,
Hutzler P., Springer N. and Schleifer K.-H.(1994) Probing
activated sludge with proteobacteria-specific
oligonucleotides:inadequary of culture-dependent methods for
describing microbial community structure. Syst. Appl.
Microbiol. 17, 405-417
70.Wagner M., Erhart R., Manz W., Amann R., Lemmer H., Wedi D.
and Schleifer K.-H.(1994) Development of an rRNA-targeted
oligonucleotide probe specific for the genus Acinetobacter
and its application for in situ monitoring in activated
sludge. Appl. Environ. Microbiol. 60(3), 792-800
71.Wagner M., Rath G., Koops H.-P., Flood J. and Amann R.
(1996) In situ analysis of nitrifying bacteria in sewage
treatment plants. Wat. Sci. Technol. 34 (1-2) , 237-244
72.Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D.,
Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C.,
Murray R.G.E., Stackebrandt E., Starr M.P. and Turper H.G.
(1987) Report of the ad hoc committee on reconciliation of
approaches to bacterial systematic.Inc. J.Syst.
Bacteriol.37, 463-464
73.Williams TM. and Unz RF.(1985) Fliamentous sulfur bacteria
of activated sludge: characterization of
Thiothrix ,Beggiatoa , and Eikelboom type 021N strains.
Appl. Environ. Microbiol. 49(4), 887-898
74.Woese C.R. (1987) Bacterial evolution. Microbiol. Rev.51,
221-271
75.Zhang X., Mandelco L. and Wiegel J. (1994) Clostridium
hydroxybenzoicum sp. nov., an amino acid-
utilizing,hydroxybenzoate-decarboxylating bacterium isolated
from methanogenic freshwater pond sediment. Int.J.
Syst.Bacteriol. 44 (2) , 214-222
76.Zhou J., Palumbo AV. and Tiedje JM.(1997) Sensitive
detection of a novel class of toluene-degrading
denitrifiers, Azoarcus tolulyticus with small-subunit rRNA
primers and probes. Appl. Environ. Microbiol. 63(6), 2384-
2390
指導教授 歐陽嶠暉(Chaio-Fuei Ouyang) 審核日期 2001-6-29
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