博碩士論文 91324015 詳細資訊




以作者查詢圖書館館藏 以作者查詢臺灣博碩士 以作者查詢全國書目 勘誤回報 、線上人數:27 、訪客IP:3.145.107.223
姓名 高立安(Li-An Kao)  查詢紙本館藏   畢業系所 化學工程與材料工程學系
論文名稱 以表面電漿共振儀研究單股去氧核醣核酸之二級結構於去氧核糖核酸雜交在動力學與反應機制的效應
(Studies of the Effect of Secondary Structure of single-stranded DNA on the Kinetics and Mechanism of Hybridizationby Surface Plasmon Resonance)
相關論文
★ 類澱粉胜肽聚集行為之電腦模擬★ 溶解度參數計算及量測於HPLC純化胜肽程序之最佳化研究
★ 利用恆溫滴定微卡計量測蛋白質分子於溶液中之第二維里係數與自我聚集之行為★ 利用SPRi探討中性DNA探針相較於一般DNA探針在低鹽雜交環境下之優勢
★ 矽奈米線場效電晶體多點之核酸檢測研究★ 使用不帶電中性核酸探針於矽奈米線場效電晶體檢測去氧核醣核酸與微核醣核酸之研究
★ 運用nDNA 修飾引子於PCR及qPCR平台以提升專一性之研究★ 設計中性DNA引子及探針以提升PCR與qPCR專一性之研究
★ 使用中性不帶電去氧核醣核酸探針於矽奈米線場效電晶體檢測微核醣核酸之研究★ 使用不帶電中性核酸探針於原位雜交技術檢測微核醣核酸之研究
★ 設計不帶電中性核酸探針於矽奈米線場效電晶體來改善富含GC鹼基核醣核酸之檢測專一性★ 合成5’-MeNPOC-2’-deoxynucleoside p-methoxy phosphoramidite以作為應用於原位合成之新穎性中性核苷酸之研究
★ 立體紙基外泌體核酸萃取裝置應用於檢測不同微環境下癌細胞所釋放之外泌體與外泌體微小核醣核酸之表現量★ 利用抗原結合區段之抗體片段探針於矽奈米線場效電晶體來改善抗原檢測濃度極限之研究
★ 利用表面電漿共振影像儀驗證最適化之抗非專一性吸附場效電晶體表面於血清環境下之免疫測定★ 使用混合自組裝單層膜於矽奈米線場效電晶體檢測微小核醣核酸之研究
檔案 [Endnote RIS 格式]    [Bibtex 格式]    [相關文章]   [文章引用]   [完整記錄]   [館藏目錄]   [檢視]  [下載]
  1. 本電子論文使用權限為同意立即開放。
  2. 已達開放權限電子全文僅授權使用者為學術研究之目的,進行個人非營利性質之檢索、閱讀、列印。
  3. 請遵守中華民國著作權法之相關規定,切勿任意重製、散佈、改作、轉貼、播送,以免觸法。

摘要(中) 表面電漿共振(Surface Plasmon Resonance,SPR)為可利用檢測光學直接偵測晶片表面上因生物反應造成折射率微量變化來達到免標定之生醫光電技術。SPR 技術具有靈敏度遠高於其他檢測方法且可排除標定的繁複過程,並且能即時地分析生物分子之間的交互作用(biomolecular interaction analysis,BIA),而大大地縮短檢測的時間並提供了更多且精確的生物資訊。近年來,已被廣泛地應用在很多生物分子診斷的領域上,諸如receptor-ligand 或抗原(antigen)與抗體(antibody)之交互作用、蛋白質分子的非特定吸附、薄膜與蛋白質間交互反應或去氧核醣核酸分子雜交(DNAhybridization)等。
本實驗室在本校機械系陳顯禎教授之協助下,自行研發的表面電漿共振系統已成功應用於各式生物分子診斷等領域,如建立完整的核酸雜交動力學研究程序,並實際檢測寡去氧核糖核酸序列雜交試驗。使用此系統於檢測、研究分子間的交互反應動力學機制可提供一套非常準確、即時的資訊。
本研究是利用表面電漿共振儀,配合DNA 分子結構預測軟體(Oligo)所計算之DNA 二級結構,來量測不同二級結構之單股DNA 於不同溫度下雜交的行為。藉此得知處於不同雜交環境以及不同的DNA 分子結構下雜交反應之動力數據,再配合質傳限制模式(Mass transport limitation model)以動力學的角度進行數據分析,並使用Van’t Hoff 方程式在DNA 雜交反應之熱力學數據對DNA 雜交反應的機制作較詳細的討論。
摘要(英) This study utilizes a surface plasmon resonance (SPR) biosensor and a theoretical secondary structure calculation program(Oligo) to investigate the influence of secondary structures of ssDNA on the DNA hybridization. It is found that the SPR angular shifts associated with the
three pairs of 60mer oligo-nucleotides with prominent secondary structures are lower than those observed for the two pairs of oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35 oC to 45 oC reduces secondary structure effects on hybridization. On the hybridization with mixture target oligo-nucleotides, the SPR results demonstrate that presence of oligo-nucleotides mixture due to non-specific interactions
between non-complementary probes and targets interfere the extent of kinetics of hybridization significantly.
In this investigation, thermodynamics information was obtained by Van’t Hoff equation and combining with the kinetics data from SPR, the
hybridization mechanism were proposed and discussed.
關鍵字(中) ★ 表面電漿共振光譜儀
★ 二級結構
★ 寡核苷酸雜交
關鍵字(英) ★ surface plasmon resonance
★ oligo-nucleotide hybridization
★ secondary structure
論文目次 第一章前言...................................................................1
第二章文獻回顧...............................................................3
2.1 表面電漿共振(Surface Plasmon Resonance,SPR) ....................3
2.1.1 表面電漿現象原理.........................................................3
2.1.2 光學激發表面電漿之方式..................................................4
2.1.3 SPR 檢測生物反應.........................................................7
2.1.4 表面電漿共振光譜儀於分子交互作用上可提供之資訊.................8
2.1.5 其他類型之表面電漿共振..................................................9
2.2 SPR 於生物檢測上之應用領域................................................15
2.2.1 蛋白質變(復)性研究......................................................15
2.2.2 DNA 與Protein 之間的交互作用.....................................16
2.2.3 Epitop Mapping..........................................................18
2.2.4 抗原(Antigen)與抗體(Antibody)與之交互作用..............19
2.2.5 生物膜(membrane)與酯雙層(lipid bilayer)......................20
2.2.6 阿茲海默症致病研究(Alzheimer) ....................................22
2.2.7 高分子膜(polymer films)研究..........................................23
2.2.8 非特異吸附生物分子交互作用-生物相容性(biocompatibility)...............24
2.2.9 組織工程...............................................................25
2.3 去氧核醣核酸及核酸雜交反應................................................28
2.3.1 去氧核醣核酸分子簡介....................................................28
2.3.2 核酸雜交反應...........................................................30
2.3.3 單股核酸之二級結構.....................................................36
第三章實驗藥品與儀器設備.....................................................38
3.1 實驗藥品.................................................................38
3.2 實驗儀器設備..............................................................40
3.2.1 表面電漿共振儀(Surface Plasmon Resonance) ...............40
3.2.2 Oligo 序列模擬軟體.....................................................44
3.3 實驗目的..............................................................46
3.4 實驗方法..................................................................46
3.4.1 實驗溶液配製..........................................................46
3.4.2 實驗步驟............................................................47
第四章結果與討論..........................................................51
4.1 Oligo 模擬核酸序列之結果..............................................51
4.2 二級結構對核酸雜交的影響.............................................59
4.2.1 具明顯二級結構雜交實驗之結果....................................59
4.2.2 非明顯二級結構雜交實驗之結果....................................61
4.3 溫度效應對二級結構核酸雜交之影響.....................................62
4.3.1 溫度效應對具明顯二級結構於核酸雜交反應之影響...62
4.3.2 溫度效應對非明顯二級結構於核酸雜交反應之影響...65
4.4 雜交反應動力學分析........................................................67
4.4.1 反應模式的選擇........................................................67
4.4.2 動力曲線擬合............................................................70
4.4.3 擬合動力學參數之合理評估............................................75
4.4.4 動力學分析...........................................................77
4.5 混合樣品之雜交反應.......................................................80
4.5.1 實驗設計................................................................80
4.5.2 混合樣品之雜交反應................................................80
4.6 雜交反應熱力學參數分析..................................................85
第五章結論...............................................................87
Reference ..................................................................90
參考文獻 Reference
[1] Ritchie R. H., "Plasma losses by fast electrons in thin films" Phys.
Rev., 1957, 106, 874
[2] Nice E.C. and B. Catimel, "Instrumental biosensors: new perspectives
for the analysis of biomolecular interactions," BioEssay, 1999, 21.4,
339-352
[3] Stenberg E., B. Persson, H. Roos and C. Urbaniczky, "Quantitative
Determination of Surface Concentration of Protein with Surface
Plasmon Resonance Using Radiolabeled Proteins," J. Colloid
Interface Sci., 1991, 143, 513-526
[4] Watts H. J., D. Yeung and H. Parkes, "Real-time Detection and
Quantification of DNA Hybridization by an Optical Biosensor," Anal.
Chem., 1995, 67, 4283-4289
[5] Minunni M, "Simultaneous determination of β2-microglobulin and
IgE using real-time biospecific interaction analysis(BIA)," Anal. Lett.,
1995, 28, 933-944
[6] Lackmann M, T. Bucci, R. J. Mann, L. A. Kravets, E. Viney, F. Smith,
R. L. Moritz, W. Carter, R. J. Simpson and N. A. Nicola,
"Purification of a ligand for the EPH-like receptor HEK using a
biosensor-based affinity detection approach," Proc Natl Acad Sci
USA, 1996, 93, 2523-2527
[7] Markgren P. O., M. Hamalainen and U. Danielson, "Screening of
compounds interacting with HIV-1 proteinase using optical biosensor
technology," Anal Biochem, 1999, 265, 340-350
[8] Zeder L. G., A. R. Neurath and M. H. Van Regenmortel, "Kinetics of
interaction between 3-hydroxyphthaloyl-beta-lactoglobulin and CD4
molecules," Biologicals, 1999, 27, 29-34
[9] Morton T. A. and D. G. Myszka, "Kinetic analysis of macromolecular
interactions using surface plasmon resonance biosensors," Methods
Enzymol, 1998, 295, 268-294
[10]Myszka D. G., M. D. Jonsen and B. J. Graves, "Equilibrium analysis
of high affinity interactions using BIACORE," Anal. Biochem., 1998,
26, 326-330.
[11]Roos H., R. Karlsson, H. Nilshans and A. Persson, "Thermodynamic
analysis of protein interactions with biosensor technology," J. Mol.
Recognit., 1998, 11, 204-210
[12]Rebecca L. R. and D. G. Myszka, "Advance in Surface plasmon
resonance biosensor analysis," Curr. Opin. Biotechnol., 2000, 11, 54-
61
[13]簡汎清, "超高解析度表面電漿共振生物感測器之研製," 碩士論文,
國立中央大學機械工程研究所, 2003
[14]Nenninger G. G., J. Homola, S. S. Yee and P. Tobiska, "Long-range
surface plasmons for high resolution surface plasmon resonance
sensors," Sensors and Actuators B, 2001, 74, 145-151
[15]Salamon Z., M. F. Brown and G. Tollin, "Plasmon resonance
spectroscopy: probing molecular interactions within membranes,"
TIBS, 1999, 24, 214-219
[16]F.-C. Chien and S.-J. Chen, "A sensitivity comparison of optical
biosensors based on four different surface plasmon resonance
modes," Biosensors and Bioelectronics, 2004, 19,
[17]Liebermann T., W. Knoll, P. Sluka and R. Herrmann, "Complement
Hybridization from Solution to Surface-Attached Probe-
Oligonucleotides Observed by Surface-Plasmon-Field-Enhanced
Fluorescence Spectroscopy" Colloids and Surfaces A:
Physicochemical and Engineering Aspects, 2000, 169, 337-350
[18]Hutter E. and M. P. Pileni, "Detection of DNA Hybridization by Gold
Nanoparticle Enhanced Transmission Surface Plasmon Resonance
Spectroscopy," The Journal of Physical Chemistry B, 2003, 107, 27,
6497-6499
[19]Lyon L. A., M. D. Musick and M. J. natan, "Colloidal Au-Enhanced
Surface Plasomn Resonance Immunosensing," Anal. Chem., 1998, 70,
5177-5183
[20]Hu W. P., S. J. Chen, K. T. Huang, J. H. Hsu, W. Y. Chen, G. L.
Chang and K. A. Lai, "A novel ultrahigh-resolution surface plasmon
resonance biosensor with an Au nanocluster-embedded dielectric
film," Biosensors and Bioelectronics, 2004, 19, 1465-1471
[21]Sota, H. and Y. Hasegawa, "Detection of conformational changes in
an immobilized protein using surface plasmon resonance," Anal.
Chem.,1998, 270, 2019-2024
[22]Gestwicki, J. E., H. V. Hsieh and J. B. Pritner, "Using receptor
conformational change to detect low molecular weight analytes by
surface plasmon resonance," Anal. Chem., 2001, 73, 5732-5737
[23]Boussaad S., J. Pean and N. J. Tao, "High-resolution
multiwavelength surface plasmon resonance spectroscopy for probing
conformational and electronic changes in redox proteins," Anal.
Chem., 2001, 72, 222-226 (2001)
[24]Ogata K., S. Morikawa and H. Nakamura, "Solution structure of a
specific DNA complex of the Myb DNA-binding domain with
cooperative recognition helices," Cell, 1994, 79, 639-648
[25]Oda M., K. Furukawa, K. Ogata, A. Sarai and H. Nakamura,
"Thermodynamics of specific and non-specific DNA binding by the
c-Myb DNA-binding domain," J. Mol. Biol., 276, 571-590
[26]Oda M., K. Furukawa, A. Sarai and H. Nakamura, "Kinetic analysis
of DNA binding by the c-Myb DNA binding domain using surface
plasmon resonance," FEBS Letters, 1999,454,288-92
[27]Oda M. and H. Nakamura, "Thermodynamic and kinetic analyses for
understanding sequence-specific DNA recognition," Genes to Cells,
2000, 5, 319-326
[28]Zeder L. G., D. Altschuh, S. Denery-Papini, J. P. Briand, G. Tribbick
and MHV. Van Regenmortel, "Epitope analysis using kinetic
measurements of antibody binding to synthetic peptides presenting
single amino acid substitutions," J. Mol. Recognition, 1993, 6, 71-79.
[29]Tarrab E., L. Berthiaume, S. Grothe, M. O'Connor-McCourt, J.
Heppell and J. Lecomte, "Evidence of a major neutralizable
conformational epitope region on VP2 of infectious pancreatic
necrosis virus, " J. Gen. Virol., 1995, 76, 551-558.
[30]Ward L. D., P. Shi and R. J. Simpson, "Binding of anti-humaninterleukin-
6 monoclonal antibodies to synthetic peptides of human
interleukin-6 studied by surface plasmon resonance," Biochem. Int.,
1992, 26, 559-565.
[31]Johne B., M. Gadnell and K. Hansen, "Epitope mapping and binding
kinetics of monoclonal antibodies by real-time biospecific interaction
analysis using surface plasmon resonance, " J. Immunol. Meth., 1993,
160, 191-198.
[32]Green R. J., R. A. Frazier, K. M. Shakesheff, M. C. Davies, C. J.
Roberts and S. J. B. Tendler, "Surface plasmon resonance analysis of
dynamic biological interactions with biomaterials," Biomaterials,
2000, 21, 1823-1835
[33]Karlsson R., A. Michaelsson and L. Mattsson, "Kinetic analysis of
monoclonal antibody-antigen interactions with a new biosensor based
analytical system," J. Immunol. Methods, 1991, 145, 229-240.
[34]Myszka D. G., T. A. Morton, M. L. Doyle and I. M. Chaiken,
"Kinetic analysis of a protein antigen antibody interaction limited by
mass transport on an optical biosensor," Biophys Chem 1997, 64,
127-l 37.
[35]Zeder-Lutz G., E. Zuber, J. Witz and R. M. H. V. Van Regenmortel,
"Thermodynamic analysis of antigen-antibody binding using
biosensor measurements at different temperatures," Anal. Biochem.,
1997, 246, 123-l 32.
[36]Oddie G. W., L. C. Gruen, G. A. Odgers, L. G. King and A. A. Kortt,
"Identification and minimization of nonideal binding effects in
BIAcore analysis: ferritin/anti-ferritin Fab' interaction as a model
system, " Anal. Biochem., 1997, 244, 301-311.
[37]Mozsolits H., S. Unabia, A. Ahmad, C. Morton, W. G. Thosmas and
M. I. Aguilar, "Electrostatic and hydrophobic forces tether the the
proximal region of the angiotensin II receptor(AT1A) carboxylterminus
to the cell membrane," Biochemistry, 2002, 41, 7830-7840
[38]Lange C. and K. W. Koch, "Calcium-dependent binding of recoverin
to membranes monitored by surface plasmon resonance spectroscopy
in real time," Biochemistry, 1997, 36, 12019-12026
[39]Danelian E., A. Karlen, R. Karlsson, S. Winiwarter, A. Hansson, S.
Lofas, H. Lennernas and M. D. Hamalainen, "SPR biosensor studier
of the direct interaction between 27 drugs and a liposome surface:
correlation with fraction absorbed in humans," Journal of Medicinal
Chemistry, 2000, 43, 2083-2086
[40]Mozsolits H., W. G. Thomas and M. I. Aguilar, "surface plasmon
resonance spectroscopy in the study of membrane-mediated cell
signalling," J. Peptide Sci., 2003, 9, 77-89
[41]Hasegawa K., K. Ono, M. Yamada and H. Naiki, "Kinetic Modeling
and Determination of Reaction Constants of Alzheimer’s β-Amyloid
Fibril Extension and Dissociation Using Surface Plasmon
Resonance," Biochemistry, 2002, 41, 13489-13498
[42]Kremer J. J. and R. M. Murphy, "Kinetics of adsorption of β-amyloid
peptide Aβ(1-40) to lipid bilayers," J. Biochem. Biophys. Methods,
2003, 57, 159-169
[43]Green R. J., S. Corneillie, J. Davies, M. C. Davies, C. J. Roberts, E.
Schacht, S. J. B. Tendler and P. M. Williams, "The investigation of
the hydration kinetics of novel PEO containing polyurethanes,"
Langmuir, 2000, 16, 2744-2750
[44]Silin V. I., G. A. Balcytis, G. N. Zhizhin and V. A. Yakovlev,
"Application of surface electromagnetic wave and surface plasmon
techniques in a protein adsorption study and sensor construction,"
Vib. Spectrosc., 1993, 5, 133-142.
[45]Silin V. I., H. Weetall and D. J. Vanderah, "SPR studies of the
nonspecific adsorption kinetics of human IgG and BSA on gold
surfaces modified by self-assembled monolayers (SAMs)," J. Colloid
Interface Sci., 1997, 185, 94-103
[46]Flamagan M. T. and R. H. Pantell, "Surface Plasmon Resonance and
Immunosensors," Eletron Lett., 1984, 20, 968-970
[47]Caruso F., D. N. Furlong and P. Kingshott, "Characterization of
ferritin adsorption onto gold," J. Colloid Interface Sci., 1997, 186,
129-140.
[48]Mrksich M., G. B. Sigal and G. M. Whitesides, "Surface plasmon
resonance permits in situ measurements of protein adsorption on self-
95
assembled monolayers of alkanethiols on gold," Langmuir, 1995, 11,
4383-4385
[49]Jordan C. E. and R. M. Corn, "Surface plasmon resonance imaging
measurements of electrostatic biopolymer adsorption onto chemically
modified gold surfaces," Anal. Chem., 1997, 69, 1449-1456
[50]Cannizzaro S. M., R. F. Padera and R. Langer, "A novel biotinylated
degradable polymer for cell-interactive applications," Biotechnol.
Bioeng., 1998, 58, 529-535.
[51]Black F. E., M. Hartshorne and M. C. Davies, "Surface engineering
and surface analysis of a biodegradable polymer with biotinylated
end groups," Langmuir, 1999, 15, 3157-3161
[52]Myszka D. G. and R. L. Rich, "Implement surface plasmon resonance
biosensors in drug discovery," PSTT, 2000, 3, 310-317
[53]McKay D. and M. J. Davies, "BIAcore, La Jolla sense new drugs,"
Trends Biotechnol. 2001, 19, 130
[54]Bier F. F., F. Kleinjung and F. W. Scheller, "Real-time Measurement
of Nucleic-Acid Hybridization Using Evanescent-Wave Sensor: steps
towards the genosensor" Sensors and Actuators B, 1997, 38-39, 78-
82
[55]Gotoh M., Y. Hasegawa, Y. Shinohara, M. Shmizu and M. Tosu, "A
New Approach to Determine the Effect of Mismatches on Kinetic
Paramemters in DNA Hybridization Using an Optical Biosensor"
DNA Research, 1995, 2, 285-293
[56]Hou M. H., S. B. Lin, J. M. P. Yuann, W. C. Lin, A. H. J. Wang and
L. S. Kan, "Effects of polyamines on the thermal stability and
formation kinetics of DNA duplexes with abnormal structure"
Nucleic Acids Res., 2001, 29, 5121-5128
[57]Persson B., K. Stenhag, P. Nilsson, A. Larsson, M. Unlen and P.
Nygren, "Analysis of Oligonucleotide Probe Affinities Using Surface
Plasmon Resonance: A Means for Mutational Scanning" Anal.
Biochem., 1997, 246, 34-44
[58]Georgiadis R., K. P. Peterlinz and A. W. Peterson, "Quantitative
Measurements and Modeling of Kinetics in Nucleic Acid Monolayer
Films Using SPR Spectroscopy," J. Am. Chem. Soc., 2000, 122,
3166-3173
[59]Peterson A. W., L. K. Wolf and R. M. Georgiadis, "Hybridization of
Mismatched or Partially Matched DNA at Surfaces," J. Am. Chem.
Soc., 124, 14601-14607
[60]M. K. Campbell (ed.), "Biochemistry3rd" (Saunders College
Publishing, Philadelphia, 1999)
[61]I. E. Alcamo (ed.), "DNA Technology2nd: The awesome skill"
(Academic, San Diego, 2001)
[62]R. F. Weaver (ed.), "Molecular Biology" (WCB McGraw-Hill,
Boston, 1999)
[63]J. D. Watson, and F. H. C. Crick, "Molecular structure of nucleic
acids: A structure for deoxyribose nucleic acid", NATURE, 1953, No.
4356
[64]Agrawal S. and R. P. Iyer, "Modified oligonucleotides as therapeutic
and diagnostic agents," Curr. Opin. Biotechnol., 1995, 6, 12-19
[65]Southern E. M., U. Maskos and J. K. Elder, "Analyzing and
comparing nucleic acid sequences by hybridization to arrays of
oligonucleotides: evaluation using experimental models," Genomics,
1992, 13, 1008-1017
[66]Okahata Y., M. Kawase, K. Niikura, F. Ohtake, H. Furusawa, and Y.
Ebara, "Kinetic Measurements of DNA Hybridization on an
Oligonucleotide-Immobilized 27-MHz Quartz Crystal Microbalance"
Anal. Chem., 1998, 70, 1288-1296
[67]Yguerabide, J. and A. Ceballos, "Quantitative Fluorescence Method
for Continuous Measurement of DNA Hybridization Kinetics Using a
Fluorescent Intercalator," Anal. Biochem., 1995, 228, 208-220
[68]Graham C. R., D. Leslie and D. J. Squirrell, "Gene probe assays on
fibre-optic evanescent wave biosensor," Biosensors Bioelectron.,
1992, 7, 487
[69]Patel D. J., A. Pardi and K. Itakura, " DNA Conformation, Dynamics,
and Interactions in Solution," science, 1982, 216, 581-590
[70]Syvanen A. C., M. Laaksonen and H. Soderlund, " Fast quantification
of nucleic acid hybrids by affinity-based hybrid collection," Nucleic
Acids Res., 1986, 14, 5037
[71]Peterlinz K. A., R. Georgiadis, T. M. Herne and M. J. Tarlov,
"Observation of Hybridization and Dehybridization of Thiol-Tethered
DNA Using Two-Color Surface Plasmon Resonance Spectroscopy," J.
Am. Chem. Soc., 1997, 119, 3401-3402
[72]Ozkan D., A. Erdem, P. Kara, K. Kerman, J. J. Gooding, P. E.
Nielsen and M. Ozsoz, "Electrochemical detection of hybridization
using peptide nucleic acids and methlene blue on self-assembled
alkanethiol monolayer modified gold electrodes," Electrochemistry
Communications, 2002, 4, 796-802
[73]Wang J., G. Liu and A. Merkoci, "Electrochemical Coding
Technology for Simultaneous Detection of Multiple DNA Targets," J.
Am. Chem. Soc., 2003, 125, 3214-3215
[74]Zeng J., A. Almadidy, J. Watterson and U. J. Krull, "Interfacial
hybridization kinetics of oligonucleotides immobilized onto fused
silica surface," Sensors and Actuators B, 2003, 90, 68-75
[75]Kambhampati D., P. E. Nielsen and W. Knoll, "Investigating the
kinetics of DNA-DNA and PNA-DNA interactions using Surface
Plasmon Resonance-Enhanced Fluorescence Spectroscopy"
Biosensors & Bioelectronics, 2001, 16, 1109-1118
[76]Wilson D. S. and J. W. Szostak, "In vitro selection of functional
nucleic acids," Annu. Rev. Biochem., 1999, 68, 611-641
[77]Holbrook J. A., M. W. Capp, R. M. Saecker and M. T. Record, Jr.,
"Enthalpy and Heat Capacity Changes for Formation of an
Oligomeric DNA Duplex : Interpretation in Terms of Coupled
Processes of Formation and Association of Single-Stranded Helices"
Biochemistry, 1999, 38, 8409-8422
[78]Kushon S. A., J. P. Jordan, J. L. Seifert, H. Nielsen, P. E. Nielsen,
and B. A. Armitage, "Effect of Secondary Structure on the
Thermodynamics and Kinetics of PNA Hybridization to DNA
Hairpins" J. Am. Chem. Soc., 2001, 123, 10805-10813
[79]Zuker M., "Mfold web server for nucleic acid folding and
hybridization prediction", Nucleic Acids Res., 2003, 31, 3406-15
[80]Davies J., Surface analytical techniques for probing biomaterial
processes, CRC Press, 1996.
[81]Karlsson R., H. Roos, L. Fagerstam, and B. Persson, "Kinetic and
Concentration Analysis Using BIA Technology" METHODS: A
Companion to Methods in Enzymology,1994, 6, 99-110
[82]Hibbert D. B., J. J. Gooding and P. Erokhin, "Kinetics of Irreversible
Adsorption with Diffusion: Application to Biomolecule
Immobilization" Langmuir, 2002, 18, 1770-1776
[83]Glaser R. W., "Antigen-Antibody Binding and Mass Transport by
Convection and Diffusion to a Surface: A Two-Dimensional
Computer Model of Binding and Dissociation Kinetics" Anal.
Biochem., 1993, 213, 152-161
[84]Schuck P. and A. P. Minton, "Analysis of Mass Transport-Limited
Binding Kinetics in Evanescent Wave Biosensors" Anal. Biochem.,
1996, 240, 262-272
[85]Pappaert K., P. V. Hummelen, J. Vanderhoeven, G. V. Baron and G.
Desmet, "Diffusion-Reaction Modeling of DNA Hybridization
Kinetics on Biochips" Chemical Engineering Science, 2003, 58,
4921-4930
[86]Karlsson R. and A. Falt, "Experimental design for kinetic analysis of
protein-protein interactions with surface plasmon resonance
biosensors" Journal of Immunological Methods; 200 (1-2): 121-133;
(1997).
[87]Southern E., K. Mir and M. Shchepinov, "Molecular Interactions on
Microarrays" nature genetics supplement, 1999, 21, 5-9
[88]Amutha R., V. Subramanian and B. U. Nair, "Free energy calculation
for DNA bases in various solvents using Flory-Huggins theory,"
Chemical Physics Letters, 2001, 335, 489-495
指導教授 陳文逸(Wen-Yih Chen) 審核日期 2004-6-17
推文 facebook   plurk   twitter   funp   google   live   udn   HD   myshare   reddit   netvibes   friend   youpush   delicious   baidu   
網路書籤 Google bookmarks   del.icio.us   hemidemi   myshare   

若有論文相關問題,請聯絡國立中央大學圖書館推廣服務組 TEL:(03)422-7151轉57407,或E-mail聯絡  - 隱私權政策聲明