三氯乙烯與四氯乙烯對人類肺癌細胞之毒性研究

以作者查詢圖書館館藏

、以作者查詢臺灣博碩士

、以作者查詢全國書目

、勘誤回報

、線上人數：35

、訪客IP：3.145.58.90

姓名

蔡欣怡(Shin-Yi Tsai) 查詢紙本館藏

畢業系所

生命科學系

論文名稱

三氯乙烯與四氯乙烯對人類肺癌細胞之毒性研究
(Toxicity of Trichloroethylene and Tetrachloroethylene to Human Lung Cancer Cells)

相關論文

★ Galectin-1 與 Thioredoxin peroxidase II 基因之選殖及表現	★ 亞砷酸鈉誘引分裂中期停滯細胞之蛋白質體研究
★ Galectin-1蛋白與亞砷酸鈉毒性與結合作用之研究	★ 氧化壓迫與p53參與三氯乙烯及四氯乙烯誘導人類肺癌細胞凋亡之研究
★ Thioredoxin Peroxidase II蛋白與亞砷酸鈉毒性與結合作用之研究	★ 環型類
★ 亞砷酸鈉與Galectin-1蛋白交互作用之研究	★ PAG蛋白質對三氧化二砷誘發急性前骨髓性白血癌細胞毒性之角色研究
★ Galectin-1蛋白對細胞生長於幾丁聚醣膜上的影響及調控機制之研究	★ 低劑量亞砷酸鈉誘引第一型血紅素氧化酶調控路徑之研究
★ 砷化物抑制Galectin-1基因之表現及機制之研究	★ 砷化物誘導Thioredoxin Peroxidase II蛋白之表現及機制之研究
★ Galectin-1蛋白促進老鼠軟骨細胞於幾丁聚醣修飾之聚乳酸-聚乙酸醇共聚物支架生長之研究	★ Galectin-1對亞砷酸鈉毒性影響之研究
★ Galectin-1誘導前脂肪細胞分化機制之研究	★ 氧化性壓迫誘導Thioredoxin peroxidase II蛋白之表現機制研究

檔案

[Endnote RIS 格式]

[Bibtex 格式]

[相關文章]

[文章引用]

[完整記錄]

[館藏目錄]

[檢視]

[下載]

本電子論文使用權限為同意立即開放。
已達開放權限電子全文僅授權使用者為學術研究之目的，進行個人非營利性質之檢索、閱讀、列印。
請遵守中華民國著作權法之相關規定，切勿任意重製、散佈、改作、轉貼、播送，以免觸法。

關鍵字(中)

★ H1299
★ 毒性研究
★ 人類肺癌細胞
★ 四氯乙烯
★ 三氯乙烯
★ H460
★ H322
★ GSH

關鍵字(英)

★ toxicity
★ GSH
★ H322
★ H460
★ H1299
★ tetrachloroethylene
★ human lung cancer cells

論文目次

中文摘要………………………………………………….I
英文摘要………………………………………………….II
目錄 …………………………………………………….III
圖表目錄 ………………………………………………VI
縮寫表…………………………………………………….VIII
第一章緒論
1.1 碳氯化物的簡介…………………………………….1
1.1.1 碳氯化物的來源與用途
1.1.2 三氯乙烯與四氯乙烯對環境之污染途徑
1.1.3 三氯乙烯之物化特性及其對人體的危害
1.1.4 四氯乙烯之物化特性及其對人體的危害.
1.2 三氯乙烯對動物之毒性……………………………..2
1.3 四氯乙烯對動物之毒性……………………………..3
1.4 三氯乙烯與四氯乙烯的代謝途徑…………………..4
1.4.1 三氯乙烯的代謝途徑
1.4.2 四氯乙烯的代謝途徑
1.5 三氯乙烯在體外培養的研究………………………..5
1.6 研究動機與目的……………………………………..5
1.6.1 研究動機
1.6.2 研究目的
第二章材料與方法
2.1 細胞株………………………………………………..7
2.2 細胞培養……………………………………………..7
2.3 藥劑處理法…………………………………………..8
2.3.1 氣相處理法
2.3.2 培養液中藥劑濃度檢測
2.4 細胞毒性檢測………………………………………...9
2.5 細胞群落分析三氯乙烯及四氯乙烯之毒性………..9
2.6 MTT細胞活性分析法……………………………..10
2.7 微核之分析…………………………………………..10
2.8 GSH之檢測………………………………………..11
2.8.1 細胞樣品前處理
2.8.2 GSH濃度測量之原理與方法
2.9 GST之活性測定……………………………………..11
2.9.1 細胞樣品前處理
2.9.2 GST活性測定之原理與方法
2.10 蛋白質定量………………………………………...12
第三章結果
3.1 培養液中揮發性有機溶劑濃度檢測………………...13
3.2 三氯乙烯及四氯乙烯對人類肺癌細胞之生長抑制作用.…………………………………………………..13
3.3 GSH對細胞之影響…………………………………...14
3.3.1 細胞密度與細胞內GSH含量之關係
3.3.2 三氯乙烯及四氯乙烯對細胞內GSH含量之影響
3.3.3 BSO抑制細胞內GSH之生成
3.3.4 細胞內GSH對三氯乙烯及四氯乙烯的毒性影響
3.4 三氯乙烯與四氯乙烯對H460細胞之GST酵素活性影響…………………………………………………...16
3.5 三氯乙烯與四氯乙烯對人類肺癌細胞之遺傳毒性...16
第四章討論 ……………………………………………..17
第五章結論與建議………………………………………20
參考文獻…………………………………………………..38
附錄………………………………………………………..45

參考文獻

Barton, H. A., C. D. Flemming and J. C. Lipscomb. (1996). Evaluating human variability in chemical risk assessment: hazard identification and dose-response assessment for noncancer oral toxicity of trichloroethylene. Toxicology. 111: 271-287.
Bernauer, U., G. Birner and W. Dekant. (1996). Biotransformation of trichloroethene: dose-dependent excretion of 2,2,2-trichloro-metabolites and mercapturic acid in rats and humans after inhalation. Arch. Toxicol. 70: 338-346.
Birner, G., S. Vamvakas, W. Dekant and D. Henschler. (1993). Nephrotoxic and genotoxic N-acetyl-S-dichlorovinyl-L-cystein is a urinary metabolite after occupational 1,1,2-trichloroethene exposure in humans: implications for the risk of trichloroethene exposure. Environ. Health Perspect. 99: 281-284.
Boyd, M. R., C. N. Statham and N. S. Longo. (1979). The pulmonary Clara cell as a target for toxic chemicals requiring metabolic activation; studies with carbon tetrachloride. J. Pharmacol. Exp. Ther. 212: 109-114.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
Bushnell, P. J. (1997). Concentration-time relationships for the effects of inhaled trichloroethylene on signal detection behavior in rats. Fundam. Appl. Toxicol. 36: 30-38.
Cohn, V. H. and J. Lyle. (1966). A fluorometric assay for glutathione. Anal. Biochem. 14: 434-440.
Cojocel, C., W. Beuter, W. Muller and D. Mayer. (1989). Lipid peroxidation: a possible mechanism of trichloroethyene-induced nephrotoxicity. Toxicology. 55: 131-141.
Dawson, B. V., P. D. Johnson, S. J. Goldberg and J. B. Ulreich. (1990). Cardiac teratogenesis of trichloroethylene and dichloroethylene in mammalian model. J. Am. Coll. Cardiol. 16: 1304-1309.
Dawson, B. V., P. D. Johnson, S. J. Goldberg and J. B. Ulreich. (1993). Cardiac teratogenesis of halogenated hydrocarbon-contaminated drinking water. J. Am. Coll. Cardiol. 21: 1466-1472.
EPA. (1985). Health assessment document for tetrachloroethylene (perchloroethylene) - final report. Washington, D.C., U.S. Environ. Prot. Agency: EPA/600/8-82/006F.
Eskenazi, B., L. Fenster, M. Hudes, A. J. Wyrobek, D. F. Katz, J. Gerson and D. M. Rempel. (1991b). A study of the effect of perchloroethylene exposure on the reproductive outcomes of wives of dry-cleaning workers. Am. J. Ind. Med. 20: 593-600.
Fahrig, R., S. Madle and H. Baumann. (1995). Genetic toxicology of trichloroethylene (TCE). Mutat. Res. 340: 1-36.
Forkert, P. G., P. L. Sylvestre and J. S. Poland. (1985). Lung injury induced by trichloroethylene. Toxicology. 35: 143-160.
Galloway, S. M., M. J. Armstrong, C. Reuben, S. Colman, B. Brown, C. Cannon, F. Nakamura, M. Ahmed, S. Duk, J. Rimpo, B. H. Margolin, M. A. Resnick, B. Anderson and E. Zeiger. (1987). Chromosome aberrations and sister chromatid exchanges in Chinese hamster ovary cells: evaluations of 108 chemicals. Environ. Mol. Mutagen. 10: 1-175.
Gazdar, A. F. (1990). Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487.
Giaccone, G. (1992). Neuromedine B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s.
Green, T., J. Dow, J. R. Foster and P. M. Hext. (1998). Formic acid excretion in rats exposed to trichloroethylene: a possible explanation for renal toxicity in long-term studies. Tixicology. 127: 39-47.
IARC. (1987). Evaluation of carcinogenic risk to humans. Suppl. 7. IARC. Lyon. 93-95.
Kligerman, A. D., M. F. Bryant, C. L. Doerr, G. L. Erexson, P. A. Evansky, P. Kwanyuen and J. K. McGee. (1994). Inhalation studies of the genotoxicity of trichloroethylene to rodents. Mutat. Res. 322: 87-96.
Lash, L. H., W. Qian, D. A. Putt, K. Desai, A. A. Elfarra, A. R. Sicuri and J. C. Parker. (1998). Glutathione conjugation of perchloroethylene in rats and mice in vitro: sex-, species-, and tissue-dependent differences. Toxicol. Appl. Pharmacol. 150: 49-57.
Lash, L. H., W. Qian, D. A. Putt, K. Jacobs, A. A. Elfarra, R. J. Krause and J. C. Parker. (1998). Glutathione conjugation of trichloroethylene in rats and mice: sex-, species-, and tissue-dependent differences. Am. Soc. Pharmacol. Exp. Ther. 26: 12-19.
Lee, T. C. and I. C. Ho. (1995). Modulation of cellular antioxidant defense activities by sodium arsenite in human fibroblasts. Arch. Toxicol. 69: 498-504.
Levine, B., M. F. Fierro and S. W. Goza. (1981). A tetrachloroethylene Fatality. J. Forensic Sci. 26: 206-209.
Lipscomb, J. C., C. M. Garrett and J. E. Snawder. (1997). Cytochrome P450-dependent metabolism of trichloroethylene: interindividual differences in humans. Toxicol. Appl. Pharmcol. 142: 311-318.
Marshall, E. K. and A. H. Owens. (1954). Absorption, excretion and metabolic fate of chloralhydrate and trichloroethanol. Bull. Johns Hopkins Hosp. 95: 1-5.
NCI. (1977). Bioassay of tetrachloroethylene for possible carcinogenicity. Washington, DC, US Govt. printing office (NCI-CG-TR-13). DHEW Publ. No. (NIH) 77-813.
Onfelt, A. (1987). Spindle disturbances in mammalian cells III. Toxicity, c-mitosis and aneuploidy with 22 different compounds. Specific and unspecific mechanisms. Mutat. Res. 182: 135-154.
Richard, S. F. and G. C. Thomas. (1994). Apoptosis or necrosis: intracellular levels of glutathione influence mode of cell death. Biochem. Pharmacol. 48: 675-681.
Robbiano, L., E. Mereto, A. M. Morando, P. Pastore and G. Brambilla. (1998). Increased frequency of micronucleated kidney cells in rats exposed to halogenated anaesthetics. Mutat. Res. 413: 1-6.
Saillenfait, A. M., I. Langonne', J. P. Sabate'. (1995). Developmental toxicity of the trichloroethylene, tetrachloroethylene and four of their metabolites in rat whole embryo culture. Arch. Toxicol. 70: 71-82.
Sujatha, T. V. and M. J. Hegde. (1998). C-mitotic effects of trichloroethylene (TCE) on bone marrow cells of mice. Mutat. Res. 413: 151-158.
Takahashi, T. (1989). p53: A frequent target for genetic abnormalities in lung cancer. Science. 246: 491-494.
Toraason, M., J. Clark, D. Dankovic, P. Mathias, S. Skaggs, C. Walker and D. Werren. (1999). Oxidative stress and DNA damage in Fisher rats following acute exposure to trichloroethylene or perchloroethylene. Toxicology. 138: 43-53.
Wang, R. S., T. Nakajima and T. Honma. (1999). Trichloroethylene inhibits aldehyde dehydrogenase only for aliphatic aldehydes of short chains in rats. Toxicology. 132: 9-18.
Wang, T. S. and H. Huang. (1994). Active oxygen species are involved in the induction of micronuclei by arsenite in XRS-5 cells. Mutagenesis. 9: 253-257.
White, A. E., S. Takehisa, E. I. Eger, S. Wolff and W. C. Stevens. (1979). Sister chromatid exchanges induced by inhaled anesthetics. Anesthesiology. 50: 426-430.
行政院勞工委員會勞工安全衛生研究所，1997，三氯乙烯暴露之尿中代謝物三氯乙酸及總三氯化合物分析方法之探討，勞工安全衛生研究報告，八十五年度研究計劃。
官知?，1998，苯環類分解菌共代謝三氯乙烯，碩士論文，中興大學環境工程學系，台中。
董天行，1998，三氯乙烯污染地下水相關清除困難之研究，碩士論文，中央大學應用地質學研究所，中壢。
陳偉立，2000，水樣中揮發性有機氣體自動化分析系統之建立及應用，科儀新知，第21卷第5期：87-94頁。
蔡文田，1992，含氯有機溶劑之毒性及新陳代謝機制，工業污染防治，第43期，第175-187頁。
環境保護署，1998，網站名稱 http://www.epa.gov.tw
鄒慶源，1997，以四氯乙烯及甲基氯仿觀測資料推估背景大氣中氫氧基濃度及其季節性變化，碩士論文，中央大學化學系，中壢。

指導教授

黃榮南(Rong-Nan Huang)

審核日期

2000-7-20

推文