博碩士論文 90224003 詳細資訊




以作者查詢圖書館館藏 以作者查詢臺灣博碩士 以作者查詢全國書目 勘誤回報 、線上人數:64 、訪客IP:3.145.51.166
姓名 劉秀玲(Hsiu-Ling Liu)  查詢紙本館藏   畢業系所 生命科學系
論文名稱 於逆境下酵母菌褪黑激素合成酶之功能分析
(Functional study of melatonin synthetic enzyme arylalkylamine N-acetyltransferase (AANAT) in yeast under stress)
相關論文
★ 陰離子界面活性劑sodium dodecylbenzene sulfonate分解菌篩選與脫磺酸酵素研究★ 鄰苯二酚加氧酵素的熱穩定性提昇研究
★ Triton X-100 分解菌之分離和分解酵素之特性研究★ Triton X-100加氧酵素之純化與定性
★ Lactobacillus reuteri於酸性與膽鹽環境中之蛋白質體研究★ 蕃茄根部受銅逆境之基因調控
★ Pseudomonas nitroreducens TX1 異化辛基苯酚聚氧乙基醇之功能性蛋白質體學:以二維電泳法分析等電點4-8之蛋白質表現★ Pseudomonas nitroreducens TX1之具耗氧活性之麩胺酸合成酶之單離
★ 人類細胞株生產含多種亞型的 干擾素-a之蛋白質體學研究★ 辛基苯酚之分解:分解菌和生物復育之菌相研究
★ 分解辛基苯酚聚氧乙基醇之耗氧酵素(二氫硫辛醯胺脫氫酶)的純化與定性★ AtNPR1轉殖番茄之性狀分析及抗病機制研究
★ Pseudomonas putida TX2分解辛基苯酚聚氧乙基醇及其具雌激素活性代謝物之研究★ 以功能性蛋白質體學研究Pseudomonas nitroreducens TX1生長於辛基苯酚聚氧乙基醇之代謝與逆境反應
★ 以功能性蛋白質體學研究Pseudomonas putida TX2生長於 辛基苯酚聚氧乙基醇與辛基苯酚之代謝與逆境反應★ 以功能性基因體學研究細菌異化辛基苯酚 聚氧乙基醇及抗逆境之基因
檔案 [Endnote RIS 格式]    [Bibtex 格式]    [相關文章]   [文章引用]   [完整記錄]   [館藏目錄]   [檢視]  [下載]
  1. 本電子論文使用權限為同意立即開放。
  2. 已達開放權限電子全文僅授權使用者為學術研究之目的,進行個人非營利性質之檢索、閱讀、列印。
  3. 請遵守中華民國著作權法之相關規定,切勿任意重製、散佈、改作、轉貼、播送,以免觸法。

摘要(中) 褪黑激素是由人類松果腺所分泌的一種荷爾蒙,不僅存在於脊椎動物,在原蟲、原生動物、真菌以及植物體中,皆發現褪黑激素的存在。但現今褪黑激素的研究只在動物中較為完整,已知褪黑激素可調節一些生理功能,例如情緒、睡眠、生物週期、免疫功能以及具有清除自由基的能力。Arylalkylamine N-acetyltransferase (AANAT) 是合成褪黑激素的關鍵酵素,而在酵母菌Saccharomyces cerevisiae中,一個AANAT的可能同源基因 (scAANAT) 已被證實具有與脊椎動物的AANAT類似之酵素活性,但此基因在酵母菌中的功能尚未明瞭,目前為止也無相關研究報導。本研究利用遺傳學方法,以scAANAT基因的大量表逹和剔除菌株作為研究材料,來探討在不同逆境下scAANAT基因在酵母菌細胞中所可能扮演之角色。結果發現,酵母菌中大量表現scAANAT基因會使其生長較遲緩。另外,當scAANAT被剔除後,酵母菌抗過氧化氫能力增強;scAANAT太過量表現時,酵母菌抗過氧化氫能力則不增反減,所以在過氧化氫逆境下,scAANAT扮演抑制生長的角色。在另一方面,scAANAT與酵母菌抗硫酸銅和氯化鈉逆境反應則無顯著關聯性。另外,本論文也利用生物資訊手法,進行資料庫搜尋及序列比對,找尋可能的植物AANAT或相關基因,結果卻找到了一群植物特有的GNAT (GCN5-related N-acetyltransferase) 類的乙醯基轉移酶,但其在植物體內真正的功能仍需進一步以分子生物學及轉殖植物研究策略加以探討。
摘要(英) Melatonin is an almost ubiquitous substance, which has been detected not only in vertebrates, but also in monera, protista, fungi, and plants. Little is known to date about its function except in vertebrates. In vertebrates, melatonin functions as a biological modulator of mood, sleep, circadian rhythms, immunologic function and free radical scavenger. Arylalkylamine N-acetyltransferase (AANAT) is the rate-limiting enzyme in melatonin synthesis. Although previous study has shown that a putative AANAT in yeast Saccharomyces cerevisiae, designated as scAANAT, possesses typical arylalkylamine N-acetyltransferase activity in vitro, the in vivo function of scAANAT remains uncharacterized. Using reverse genetic approach, this work aimed to study possible role of scAANAT in growth and stress response of yeast. Unexpectedly, the results showed that over-dose expression of scAANAT in yeast led to growth retardation under normal growth condition and deduction of resistance to hydrogen peroxide stress. On other hand, scAANAT is not involoved in yeast response to copper and salinity stresses. In addition, we employed a bioinformatic approach to explore putative plant AANAT homologous genes, and a group of novel GNATs unique in plants has been identified by database search. The results and possible studies necessary for further elucidating the role of melatonin and AANATs in growth and stress response in yeast and plants are discussed.
關鍵字(中) ★ 植物
★ GNAT
★ 酵母菌
★ 逆境
★ AANAT
★ 褪黑激素
關鍵字(英) ★ GNAT
★ plant
★ stress
★ yeast
★ AANAT
★ melatonin
論文目次 中文摘要…………………………………..……………………………….…Ⅰ
英文摘要………………………………………………………………………II
目錄……………………………………..…………………………………….III
圖表目錄、附錄目錄…………………..…………………………….....….…IV
縮寫與全名對照表…………………..………………………………….……VI
壹、 緒論…………………………………….……………………..…………..1
貳、 內文
第一章 於逆境下酵母菌褪黑激素合成酶之功能分析
一、 前言………………………………………………….………….6
二、 材料與方法………………………………………………..……8
三、 結果……………………………………………………………18
四、 討論……………………………………………………………22
五、 圖表……………………………………………………………28
第二章 以生物資訊法搜尋植物中酵母菌褪黑激素合成酶之可能同源基因
一、 前言……………………………………………………………51
二、 材料與方法……………………………………………………53
三、 結果與討論……………………………………………………55
四、 圖表……………………………………………………………59
參、 結論……………………………………………………………………...67
肆、 參考文獻………………………………………………………………...68
伍、 附錄…………………………………………………………………...…73
參考文獻 尤曉莉 (2002) 小葉浮萍之色胺酸合成酶β鏈基因選殖與色胺酸及其衍生物受銅影響之化學分析。國立中央大學碩士論文
Amherd R, Hintermann E, Walz D, Affolter M, Meyer UA (2000) Purification, cloning, and characterization of a second arylalkylamine N-acetyltransferase from Drosophila melanogaster. DNA Cell Biol 19: 697-705
Amherd R, Hintermann E, Walz D, Affolter M, Meyer UA, Coto-Montes A, Hardeland R (2000) Purification, cloning, and characterization of a second arylalkylamine N-acetyltransferase from Drosophila melanogaster Antioxidative effects of melatonin in Drosophila melanogaster: antagonization of damage induced by the inhibition of catalase. DNA Cell Biol 19: 697-705
Atmane N, Dairou J, Paul A, Dupret JM, Rodrigues-Lima F (2003) Redox regulation of the human xenobiotic metabolizing enzyme arylamine N-acetyltransferase 1 (NAT1). Reversible inactivation by hydrogen peroxide. J Biol Chem 278: 35086-35092
Avery SV, Howlett NG, Radice S (1996) Copper toxicity towards Saccharomyces cerevisiae: dependence on plasma membrane fatty acid composition. Appl Environ Microbiol 62: 3960-3966
Benitez-King G, Tunez I, Bellon A, Ortiz GG, Anton-Tay F (2003) Melatonin prevents cytoskeletal alterations and oxidative stress induced by okadaic acid in N1E-115 cells. Exp Neurol 182: 151-159
Beyer CE, Steketee JD, Saphier D (1998) Antioxidant properties of melatonin--an emerging mystery. Biochem Pharmacol 56: 1265-1272
Blomberg A (2000) Metabolic surprises in Saccharomyces cerevisiae during adaptation to saline conditions: questions, some answers and a model. FEMS Microbiol Lett 182: 1-8
Brzezinski A (1997) Melatonin in humans. N Engl J Med 336: 186-195
Burke TW, Cook JG, Asano M, Nevins JR (2001) Replication factors MCM2 and ORC1 interact with the histone acetyltransferase HBO1. J Biol Chem 276: 15397-15408
Chan HM, Krstic-Demonacos M, Smith L, Demonacos C, La Thangue NB (2001) Acetylation control of the retinoblastoma tumour-suppressor protein. Nat Cell Biol 3: 667-674
Chen G, Huo Y, Tan DX, Liang Z, Zhang W, Zhang Y (2003) Melatonin in Chinese medicinal herbs. Life Sci 73: 19-26
Coon SL, Roseboom PH, Baler R, Weller JL, Namboodiri MA, Koonin EV, Klein DC (1995) Pineal serotonin N-acetyltransferase: expression cloning and molecular analysis. Science 270: 1681-1683
Coon SL, Weller JL, Korf HW, Namboodiri MA, Rollag M, Klein DC (2001) cAmp regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation. J Biol Chem 276: 24097-24107
Costa V, Moradas-Ferreira P (2001) Oxidative stress and signal transduction in Saccharomyces cerevisiae: insights into ageing, apoptosis and diseases. Mol Aspects Med 22: 217-246
De Freitas J, Wintz H, Kim JH, Poynton H, Fox T, Vulpe C (2003) Yeast, a model organism for iron and copper metabolism studies. Biometals 16: 185-197
Dyda F, Klein DC, Hickman AB (2000) GCN5-related N-acetyltransferases: a structural overview. Annu Rev Biophys Biomol Struct 29: 81-103
Felsenstein J (1997) PHYLIP package 3.6. University of Washington, Seattle
Ganguly S, Coon SL, Klein DC (2002) Control of melatonin synthesis in the mammalian pineal gland: the critical role of serotonin acetylation. Cell Tissue Res 309: 127-137
Ganguly S, Gastel JA, Weller JL, Schwartz C, Jaffe H, Namboodiri MA, Coon SL, Hickman AB, Rollag M, Obsil T, Beauverger P, Ferry G, Boutin JA, Klein DC (2001) Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis. Proc Natl Acad Sci U S A 98: 8083-8088
Ganguly S, Mummaneni P, Steinbach PJ, Klein DC, Coon SL (2001) Characterization of the Saccharomyces cerevisiae homolog of the melatonin rhythm enzyme arylalkylamine N-acetyltransferase (EC 2.3.1.87). J Biol Chem 276: 47239-47247
Giaever G, Chu AM, Ni L, Connelly C, Riles L, Veronneau S, Dow S, Lucau-Danila A, Anderson K, Andre B, Arkin AP, Astromoff A, El-Bakkoury M, Bangham R, Benito R, Brachat S, Campanaro S, Curtiss M, Davis K, Deutschbauer A, Entian KD, Flaherty P, Foury F, Garfinkel DJ, Gerstein M, Gotte D, Guldener U, Hegemann JH, Hempel S, Herman Z, Jaramillo DF, Kelly DE, Kelly SL, Kotter P, LaBonte D, Lamb DC, Lan N, Liang H, Liao H, Liu L, Luo C, Lussier M, Mao R, Menard P, Ooi SL, Revuelta JL, Roberts CJ, Rose M, Ross-Macdonald P, Scherens B, Schimmack G, Shafer B, Shoemaker DD, Sookhai-Mahadeo S, Storms RK, Strathern JN, Valle G, Voet M, Volckaert G, Wang CY, Ward TR, Wilhelmy J, Winzeler EA, Yang Y, Yen G, Youngman E, Yu K, Bussey H, Boeke JD, Snyder M, Philippsen P, Davis RW, Johnston M (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418: 387-391
Grunstein M (1997) Histone acetylation in chromatin structure and transcription. Nature 389: 349-352
Hardeland R (1999) Melatonin and 5-methoxytryptamine in non-metazoans. Reprod Nutr Dev 39: 399-408
Harumi T, Matsushima S (2000) Separation and assay methods for melatonin and its precursors. J Chromatogr B 747: 95-110
Howarth JR, Dominguez-Solis JR, Gutierrez-Alcala G, Wray JL, Romero LC, Gotor C (2003) The serine acetyltransferase gene family in Arabidopsis thaliana and the regulation of its expression by cadmium. Plant Mol Biol 51: 589-598
Iizuka M, Stillman B (1999) Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein. J Biol Chem 274: 23027-23034
Ikura T, Ogryzko VV, Grigoriev M, Groisman R, Wang J, Horikoshi M, Scully R, Qin J, Nakatani Y (2000) Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis. Cell 102: 463-473
Jones RP, Greenfield PF (1984) A review of yeast ionic nutrition. I. Growth and fermentation requirements. Process Biochem: 48-60
Klein DC, Coon SL, Roseboom PH, Weller JL, Bernard M, Gastel JA, Zatz M, Iuvone PM, Rodriguez IR, Begay V, Falcon J, Cahill GM, Cassone VM, Baler R (1997) The melatonin rhythm-generating enzyme: molecular regulation of serotonin N-acetyltransferase in the pineal gland. Recent Prog Horm Res 52: 307-357; discussion 357-308
Kolar J, Machackova I, Eder J, Prinsen E, Dongen WV, Onckelen HV, Illnerova H (1997) Melatonin: occurrence and daily rhythm in Chenopodium rubrum. Phytochem 44: 1407-1413
Madeo F, Frohlich E, Frohlich KU (1997) A yeast mutant showing diagnostic markers of early and late apoptosis. J Cell Biol 139: 729-734
Manchester LC, Tan DX, Reiter RJ, Park W, Monis K, Qi W (2000) High levels of melatonin in the seeds of edible plants: possible function in germ tissue protection. Life Sci 67: 3023-3029
Martinez-Balbas MA, Bauer UM, Nielsen SJ, Brehm A, Kouzarides T (2000) Regulation of E2F1 activity by acetylation. Embo J 19: 662-671
McGarry RC, Barron YD, Carvalho MF, Hill JE, Gold D, Cheung E, Kraus WL, Lazarowitz SG (2003) A novel Arabidopsis acetyltransferase interacts with the geminivirus movement protein NSP. Plant Cell 15: 1605-1618
McMurry MT, Krangel MS (2000) A role for histone acetylation in the developmental regulation of VDJ recombination. Science 287: 495-498
Mizzen CA, Allis CD (1998) Linking histone acetylation to transcriptional regulation. Cell Mol Life Sci 54: 6-20
Neuwald AF, Landsman D (1997) GCN5-related histone N-acetyltransferases belong to a diverse superfamily that includes the yeast SPT10 protein. Trends Biochem Sci 22: 154-155
Nguyen-nhu NT, Knoops B, Puig S, Lee J, Lau M, Thiele DJ (2002) Alkyl hydroperoxide reductase 1 protects Saccharomyces cerevisiae against metal ion toxicity and glutathione depletion Biochemical and genetic analyses of yeast and human high affinity copper transporters suggest a conserved mechanism for copper uptake. Toxicol Lett 135: 219-228
Pandey R, Muller A, Napoli CA, Selinger DA, Pikaard CS, Richards EJ, Bender J, Mount DW, Jorgensen RA (2002) Analysis of histone acetyltransferase and histone deacetylase families of Arabidopsis thaliana suggests functional diversification of chromatin modification among multicellular eukaryotes. Nucleic Acids Res 30: 5036-5055
Poeggeler B, Thuermann S, Dose A, Schoenke M, Burkhardt S, Hardeland R (2002) Melatonin's unique radical scavenging properties - roles of its functional substituents as revealed by a comparison with its structural analogs. J Pineal Res 33: 20-30
Prior C, Potier S, Souciet J-L, Sychrova H (1996) Characterization of the NHA1 gene encoding a Na+/H+ -antiporter of the yeast Saccharomyces cerevisiae. FEBS Lett 387: 89-93
Reiter RJ, Tan DX (2002) Melatonin: an antioxidant in edible plants. Ann N Y Acad Sci 957: 341-344
Reiter RJ, Tan DX, Osuna C, Gitto E (2000) Actions of melatonin in the reduction of oxidative stress. A review. J Biomed Sci 7: 444-458
Rodrigues-Lima F, Delomenie C, Goodfellow GH, Grant DM, Dupret JM (2001) Homology modelling and structural analysis of human arylamine N-acetyltransferase NAT-1: evidence for the conservation of a cysteine protease catalytic domain and an active-site loop. Biochem J 356: 327-334
Roseboom PH, Coon SL, Baler R, McCune SK, Weller JL, Klein DC (1996) Melatonin synthesis: analysis of the more than 150-fold nocturnal increase in serotonin N-acetyltransferase messenger ribonucleic acid in the rat pineal gland. Endocrinology 137: 3033-3045
Serrano R, Rodriguez-Navarro A (2001) Ion homeostasis during salt stress in plants. Curr Opin Cell Biol 13: 399-404
Siraki AG, O'Brien PJ (2002) Prooxidant activity of free radicals derived from phenol-containing neurotransmitters. Toxicology 177: 81-90
Soares EV, Hebbelinck K, Soares HM (2003) Toxic effects caused by heavy metals in the yeast Saccharomyces cerevisiae: a comparative study. Can J Microbiol 49: 336-343
Soutoglou E, Katrakili N, Talianidis I (2000) Acetylation regulates transcription factor activity at multiple levels. Mol Cell 5: 745-751
Spilianakis C, Papamatheakis J, Kretsovali A (2000) Acetylation by PCAF enhances CIITA nuclear accumulation and transactivation of major histocompatibility complex class II genes. Mol Cell Biol 20: 8489-8498
Sprenger J, Hardeland R, Fuhrberg B, Han S-Z (1999) Melatonin and other 5-methoxylated indoles in yeast: presence in high concentrations and dependence on tryptophan availability. Cytologia 64: 209-213
Tan DX, Chen LD, Poeggeler B, Manchester LC, Reiter RJ (1993) Melatonin: a potent endogenous, hydroxyl radical scavenger. Endocrine J 1: 57-60
Tatusova TA, Madden TL (1999) BLAST 2 Sequences, a new tool for comparing protein and nucleotide sequences. FEMS Microbiol Lett 174: 247-250
Van Tassel DL, Roberts N, Lewy A, O'Neill SD (2001) Melatonin in plant organs. J Pineal Res 31: 8-15
Witt-Enderby PA, Bennett J, Jarzynka MJ, Firestine S, Melan MA (2003) Melatonin receptors and their regulation: biochemical and structural mechanisms. Life Sci 72: 2183-2198
Zhao Y, Lin YH (2003) Growth of Saccharomyces cerevisiae in a chemostat under high glucose conditions. Biotechnol Lett 25: 1151-1154
指導教授 詹明才、黃雪莉
(Ming-Tsair Chan、Shir-Ly Huang)
審核日期 2004-1-27
推文 facebook   plurk   twitter   funp   google   live   udn   HD   myshare   reddit   netvibes   friend   youpush   delicious   baidu   
網路書籤 Google bookmarks   del.icio.us   hemidemi   myshare   

若有論文相關問題,請聯絡國立中央大學圖書館推廣服務組 TEL:(03)422-7151轉57407,或E-mail聯絡  - 隱私權政策聲明