近場電紡絲技術應用於排列多種密度、異向性之奈米纖維結構以引導細胞擴散及型態之研究

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姓名

何哲宇(Che-yu Ho) 查詢紙本館藏

畢業系所

機械工程學系

論文名稱

近場電紡絲技術應用於排列多種密度、異向性之奈米纖維結構以引導細胞擴散及型態之研究
(Guided cell morphology and migration on near-field eletrospun nanofiber-textured substrates with variable local density and anisotropy)

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摘要(中)

本篇論文講述近場電紡絲技術已證明能夠將褐藻酸鈉/聚氧化乙烯奈米纖維沉積成規定的圖案及定位密度以觀察平行排列的奈米纖維間距在10、20、40、80μm以及利用近場電紡絲原地堆疊的技術將纖維堆疊成3D的奈米纖維結構。並且觀察當纖維以不同密度及不同堆疊高度培養細胞時對於細胞型態，擴散及增殖的影響。利用快速傅立葉轉換(FFT)測量細胞的生長方向及對齊程度，並且利用細胞計數盤計算細胞數量，用以觀察研究細胞之生長方向與奈米纖維之間的排列方式以及纖維堆疊的高度有重大的影響。這些低成本、快速製作的製程未來可以有效運用於細胞貼附、擴散等議題。

摘要(英)

This thesis mainly research formation of nanofiber, controlled technology and application in near-field electrospinning. The focus of the study is Direct-write, highly-aligned Alginate-poly(ethylene oxide) nanofiber patterns for cell architecture/ morphology and spreading control. Near-field electrospinning (NFES) has been demonstrated to be able to achieve direct-write and highly-aligned Alginate nanofibers (ANF) with prescribed positioning density. Cells spreading in preferential direction could be observed on parallel aligned nanofibers and the ANF patterns were capable of guiding cell extension, when the distances between them are 10、20、40、80μm, respectively. Alignment of the cells was characterized by elongation and orientation of the cells via the FFT data and. Parallel ANF indicates the alignment values sequentially increased as a function of the positioning density such that incrementally more aligned cells were closely related to the increasing ANF positioning density. And the use of repeated stacking technology to produce three-dimensional nano-fiber structure to observe the fiber for cell reproduction and diffusion These maskless, low-cost and direct-write patterns can be facility fabricated and will be a promising tool to study the cell-based research such as cell adhesion, spreading and tissue architecture.

關鍵字(中)

★ 近場電紡絲技術
★ 選擇性沉積
★ 電壓調節
★ 細胞擴散
★ 褐藻酸鈉

關鍵字(英)

★ Near-field electrospinning
★ Selective deposition
★ differential voltage regulation
★ cells spreading
★ alginate

論文目次

Content
摘要 I
Abstract II
誌謝 IV
目錄 V
圖目錄 VIII
第一章緒論 1
1-1 電紡絲技術 1

1-2 褐藻酸鈉/聚氧化乙烯 1

1-3近場電紡絲技術達到穩定、準確排列的選擇性沉積 2

1-4近場電紡絲技術利用原地沉積堆疊纖維支架 2

1-5 論文架構 3

第二章可控制大面積奈米纖維沉積及排列用於影響細胞型態擴散及繁殖 4
2-1 導論 4
2-2 實驗 4
2-2-1 電紡絲溶液 4

2-2-2 電紡絲設備架構 5

2-2-3聚吡咯基底 5

2-2-4細胞培養 5

2-2-5細胞排列之量化 5

2-2-5細胞數量計算 6
2-3 結果與討論 6
第三章利用堆疊方式增加纖維高度以加強平行排列纖維對細胞型態與增殖的影響 17
3-1 導論 17
3-2 實驗 18
3-2-1 電紡絲溶液 18

3-2-2 電紡絲設備架構 18

3-2-3聚吡咯基底 18

3-2-4細胞培養 19

3-2-5細胞排列之量化 19

3-2-6細胞數量計算 19
3-3 結果與討論 20
第五章結論 32
參考文獻 33
附錄 37

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指導教授

傅尹坤(Yiin-kuen Fuh)

審核日期

2014-8-28

推文