The Rheological Properties of Invasive Cancer Cells

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姓名

吳治謙(Zhi-Qian Wu) 查詢紙本館藏

畢業系所

生物物理研究所

論文名稱

(The Rheological Properties of Invasive Cancer Cells)

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摘要(中)

本研究目的在於探討不同侵犯能力的癌細胞其流變學的特性有何差異。我們以原子力顯微術量測細胞的楊氏模量。結果顯示侵犯能力越強的癌細胞，其彈性有越軟的趨勢。我們也量測了細胞的複變剪切模量，並藉此求出消耗正切。結果顯示侵犯能力越強的癌細胞，其細胞質有較偏黏彈性液體的傾向。細胞質對探針的擾動情形也有被量測。從力變化功譜及探針偏折的時間序列數據，我們得知侵犯能力較強的癌細胞其細胞質內的主動粒子的碰撞頻率比較高，而且主動粒子在運動上的協同性會較強。最後我們從探針偏折的時間序列數據求出的自相關函數的變化形式，推知侵犯性較強的癌細胞其粒子運動的弛豫時間較短。

摘要(英)

The purpose of this study is to investigate the rheological properties of invasive cancer cells. We use atomic force microscopy to measure the Young’s modulus of different cell types. The result shows that the more invasive the cancer cells are, the softer they will be. We also measure the complex shear modulus, and use it to calculate the loss tangent. The result shows that if the cancer cells are more invasive, they will have the tendency to be viscoelastic liquid. The cantilever perturbation by cytoplasm is measured. From the force power spectrum and the time series data of cantilever deflection, we infer that the collision frequency of active particles is higher and the cooperativity of active motion is stronger in the more invasive cancer cells. Finally, we calculate the autocorrelation function from cantilever deflection time series data and perform the model fitting. The results show that relaxation time is shorter in the more invasive cancer cells.

關鍵字(中)

★ 原子力顯微術

關鍵字(英)

論文目次

摘要…………………………………………………………………………………………………………………………..i
Abstract…………………………………………………………………………………………………………………….ii
Contents…………………………………………………………………………………………………………………..iii
List of figures…………………………………………………………………………………………………………....v
Chapter 1. Introduction……………………………………………………………1
1.1 motivation………………………………………………………………………..1
1.2 literature review…………………………………………………………………2
1.2.1 elastic and viscoelastic measurement with AFM………………………….2
1.2.2 mechanical vibration of yeast’s cell wall……………………………………3
1.3 background………………………………………………………………………4
1.2.1 AFM principles………………………………………………………………...4
1.2.2 AFM operation modes………………………………………………………..5
1.2.3 Hertz model……………………………………………………………………7
1.2.4 complex shear modulus……………………………………………………...8
Chapter 2. Materials and methods…………………………………………….10
2.1 agar preparation……………………………………………………………….10
2.2 cell culture condition…………………………………………………………..10
2.2.1 3T3 fibroblasts…………………………………………………………….....10
2.2.2 LoVo cells…………………………………………………………………….10
2.2.3 SW620 cells………………………………………………………………….10
2.2.4 HCT116 cells………………………………………………………………...11
2.3 force measurement……………………………………………………………11
2.3.1 sensitivity calibration………………………………………………………..11
2.3.2 spring constant calibration………………………………………………....11
2.4 image scanning………………………………………………………………..12
2.5 Young′s modulus measurement……………………………………………..13
2.6 complex shear modulus measurement……………………………………...14
2.7 cantilever perturbation………………………………………………………...14
2.7.1 constant height mode……………………………………………………….14
2.7.2 signal processing…………………………………………………………....14
2.7.3 power spectrum density…………………………………………………….15
2.7.4 total power, average power, and spectral entropy……………………….15
Chapter 3. Results and discussions…………………………………………..16
3.1 elastic property of glass, Petri dish, and agar……………………………….16
3.2 viscoelastic property of 5% agar……………………………………………..19
3.3 elastic property of 3T3 fibroblast, LoVo, SW620, and HCT116…………..21
3.4 viscoelastic property of 3T3 fibroblast, LoVo, SW620, and HCT116…….25
3.5 mechanical perturbation of cytoplasm to the AFM tip……………………..27
3.5.1 force power spectrum of 1% agar, SW620, and HCT116……………….27
3.5.2 active particles activity………………………………………………………30
3.5.3 autocorrelation function fitting……………………………………………...38
Chapter 4. Conclusions and prospects………………………………………41
References………………………………………………………….....................42

參考文獻

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指導教授

黎璧賢

審核日期

2015-11-13

推文