探討以PDMS海綿萃取發酵系統連續生產乙醇之研究

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許瑞純(Jui-Chun Hsu) 查詢紙本館藏

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化學工程與材料工程學系

論文名稱

探討以PDMS海綿萃取發酵系統連續生產乙醇之研究
(Improving bio-ethanol production of Saccharomyces cerevisiae by continuous extractive fermentation system using PDMS sponge)

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摘要(中)

全球暖化已經成為目前地球所面臨的最大問題，其中佔很大一部分的原因為石化燃料的大量使用，導致溫室氣體的排放量不斷上升。為了降低人類對於石化燃料的使用率，興起研究可再生的生質燃料。生質燃料中最主要的就是利用酵母菌發酵生產的生質乙醇。
近年來，燃料乙醇用來取代汽油的使用已越來越被重視與廣泛使用。但過去研究指出，S. cerevisiae發酵生產乙醇時，乙醇體積在發酵液中達到¬4 vol %至10 vol%，乙醇就會反過來對菌體生長產生抑制，導致S. cerevisiae無法生產乙醇，甚至死亡。本篇研究所使用的Saccharomyces cerevisiae BCRC 21812之乙醇耐受性為8.87 vol%。
本研究設計一個新的生物反應器－Polydimethylsiloxane (PDMS)海綿連續式萃取發酵系統(continuous extractive fermentation system by PDMS sponge)。PDMS海綿在此處為萃取劑的角色，利用擠壓海綿進行吸附脫附的過程，將發酵液中乙醇濃度降低，避免tolerance發生，進而提升產率及乙醇濃度。PDMS海綿連續式萃取發酵系統可移除水溶液中50%以上的乙醇量。發酵液經過連續十次的萃取，相較於批次系統可提升21.07%的總乙醇量。

摘要(英)

Global warming has become the biggest problem currently facing the earth, which accounted for a large part of the reason for the extensive use of fossil fuels. In order to reduce the use of fossil fuels, and began to study other alternative sources of energy, so the rise of renewable biofuels. Biofuel is one of the main use of yeast fermentation production of ethanol.
In recent years, the fuel ethanol replaces gasoline that has been extensively used and attention. Yeast strains of Saccharomyces cerevisiae have been extensively studied for bio-ethanol. Previous studies have indicated that the S. cerevisiae fermentation to produce ethanol, the volume of ethanol in the fermentation broth reached 4 vol% to 10 vol%, ethanol will in turn on cell growth inhibition, leading to S. cerevisiae was unable to produce ethanol, and even death. Saccharomyces cerevisiae BCRC 21812 of ethanol tolerance was 8.87 vol% in this study.
In order to avoid the occurrence of tolerance, a novel bioreactor has been developed, continuous extractive fermentation system by polydimethylsiloxane (PDMS) sponge. PDMS sponge is extracting agent, and the adsorption and desorption of ethanol is extruded by sponge. This system can remove more than 50% of ethanol in ethanol solution. After ten successive extraction, the medium was increased the total amount of ethanol by 21.07% compared to batch.

關鍵字(中)

★ 乙醇
★ Saccharomyces cerevisiae
★ PDMS

關鍵字(英)

論文目次

摘要 I
Abstract II
致謝 III
目錄 IV
表目錄 VIIII
圖目錄 X
第一章緒論 1
1.1 研究動機 1
1.2 研究目的 2
第二章文獻回顧 4
2.1 再生能源 4
2.1.1 再生能源之介紹 4
2.1.2 生質能之發展 5
2.2 生質乙醇 7
2.2.1 生質乙醇之介紹 7
2.2.2 生質乙醇發展與現況 8
2.2.3 生質乙醇之研究重點 11
2.3 酵母菌（Saccharomyces cerevisiae） 15
2.3.1. 酵母菌之基本介紹 15
2.3.2. 酵母菌之生殖方式 16
2.3.3. 酵母菌之代謝作用 17
2.3.4. 酵母菌之生長條件 19
2.3.5. 酵母菌之抑制因子 19
2.4 萃取發酵 20
2.4.1 萃取發酵之介紹 20
2.4.2 萃取方法之介紹及發展 20
2.4.3 萃取發酵之應用 22
2.5 聚二甲基矽氧烷海綿 26
2.5.1 聚二甲基矽氧烷之介紹 26
2.5.2 聚二甲基矽氧烷海綿之介紹及應用 28
第三章實驗規劃、材料與方法 30
3.1 實驗規劃 30
3.2 實驗材料、設備與裝置 32
3.2.1 實驗菌株 32
3.2.2 實驗藥品 32
3.2.3 實驗儀器與設備 33
3.2.4 數據使用參數設定 36
3.2.5 實驗裝置 37
3.3 實驗方法 40
3.3.1 菌種保存 40
3.3.2 培養基組成 41
3.3.3 Saccharomyces cerevisiae生長因素之探討 43
3.3.4 Saccharomyces cerevisiae發酵系統之建立 44
3.3.5 PDMS海綿製備 45
3.3.6 PDMS海綿對於菌體之毒性測試 46
3.3.7 PDMS海綿連續式萃取發酵系統的應用 46
3.4 分析方法 49
3.4.1 細胞乾重及細胞密度對光學密度之檢量線 49
3.4.2 葡萄糖殘量測定 50
3.4.3 乙醇濃度分析 52
第四章實驗結果與討論 54
4.1 批次發酵生產乙醇 54
4.1.1 厭氧發酵－碳源濃度對Saccharomyces cerevisiae發酵動力曲線之影響 54
4.1.2 厭氧發酵－Saccharomyces cerevisiae之乙醇耐受性測試 ………………………………………………………...57
4.1.3 好氧發酵－通氣量對Saccharomyces cerevisiae發酵動力曲線之影響 59
4.2 兩階段式發酵 62
4.2.1 好氧與厭氧發酵方式之比較 62
4.2.2 好氧時間長度之選擇 64
4.2.3 發酵方式不同對Saccharomyces cerevisiae發酵之影響 ………………………………………………………..66
4.3 PDMS海綿連續式萃取發酵系統 68
4.3.1 PDMS海綿毒性測試 68
4.3.2 連續式萃取發酵系統之測試 70
4.3.3 萃取在饋料發酵之影響 71
4.3.4 萃取後添加饋料條件之選擇 74
4.3.5 起始萃取時機的選擇 78
第五章結論與建議 83
5.1 結論 83
5.2 建議 84
第六章參考文獻 86

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指導教授

徐敬衡(Chin-Hang Shu)

審核日期

2016-8-25

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