綠茶表沒食子酸酯型兒茶素酸酯在3T3-L1脂肪細胞分化過程中調節FoxO家族基因的表現

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陳毅(Yi Chen) 查詢紙本館藏

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生命科學系

論文名稱

綠茶表沒食子酸酯型兒茶素酸酯在3T3-L1脂肪細胞分化過程中調節FoxO家族基因的表現
(Green tea epigallocatechin gallate regulates the expression of the FoxO family during differentiation of 3T3-L1 fat cells)

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摘要(中)

Forkhead box class O (FoxO)家族基因包含有FoxO1、FoxO3、FoxO4和FoxO6四種，過去有許多文獻分別指出各FoxO基因在細胞內調控了包括分化、葡萄醣代謝、DNA修復和細胞凋亡在內的許多功能。在本實驗中我們使用了3T3-L1前脂肪細胞分化為脂肪細胞的系統，透過處理Dexamethasone、Insulin和3-isobutyl-1-methylxanthine等3種藥物所組成之分化劑，促使細胞進行為期14天的分化。在分化的過程中，細胞的數目有明顯的增加，而細胞內會有三酸甘油脂的堆積。在我們的實驗中發現，相較於沒有處理EGCG的組別，在分化期間處理EGCG會使細胞內油滴的數量減少。當沒有處理EGCG時，細胞的FoxO1、FoxO3、FoxO4和FoxO6的mRNA表現量在分化過程的第2~6天之間會有增加的趨勢，而在第6~14天之間的表現量則會有降低的趨勢。在14天的分化期間，EGCG會大幅降低FoxO1、FoxO3、FoxO4和FoxO6的mRNA表現量。此外ATF-3的mRNA表現量則會有增加的趨勢，其表現量並不會受到EGCG的影響。這些實驗結果顯示EGCG會透過調節FoxO的表現量去影響3T3-L1前脂肪細胞的分化。

摘要(英)

The forkhead box class O (FoxO) family of mammals contained 4 members: FoxO1, FoxO3, FoxO4 and FoxO6. Previous studies have revealed that FoxO genes regulate many functions in cells, including differentiation, glucose metabolism, DNA repair and apoptosis. In this study, we used the preadipocyte-adipocyte differentiation system of 3T3-L1 cells, induced by dexamethasone, insulin and 3-isobutyl-1-methylxanthine for a period of 14 days. During the differentiation, the cell number increased, and the TG droplet increased in cells. Our study also found that the TG droplet decreased when 3T3-L1 cells were treated EGCG, compared to control. In the absence of EGCG, FoxO1, FoxO3, FoxO4, and FoxO6 tended to increase their mRNA expressions from Day 2 to Day 6 of differentiation and decrease from Day 6 to Day 14 of differentiation. EGCG, at 100 μM, but not at 25 or 50 μM, significantly decreased levels of FoxO1, FoxO3, FoxO4, and FoxO6 mRNAs during a 14-day period of differentiation. Moreover, activating transcription factor (ATF)-3 tended to increase its mRNA expression and was not altered by EGCG treatment. These data suggest that EGCG regulates 3T3-L1 adipocyte differentiation through modulations of FoxO but not ATF-3 levels.

關鍵字(中)

★ 脂肪細胞
★ 兒茶素
★ 細胞分化

關鍵字(英)

★ FoxO
★ EGCG
★ adipocyte
★ differentiation
★ ATF-3

論文目次

電子檔授權書……………………………………………………………………………i
紙本延後上架申請書……………………………………………………………..…….ii
推薦書…………………………………………………………………………….…….iii
審定書…………………………………………………………………………….…….iv
中文摘要………………………….……………………………………………………v
英文摘要……………………….………………...……………………………………..vi
致謝…………………………………………….………………………………………vii
使用藥劑之縮寫與全名對照表………………...…….………………………………viii
目錄…………………………………………………..……………………………….ix
一、前言……………………………………………….……………………….……..1
I. FoxO轉錄因子…………………………………..…………….…………….1
II. ATF-3轉錄因子……………...……………………………………….…….3
III. 脂肪細胞與細胞分化………………………………………….……………..3
IV. 綠茶兒茶素EGCG……………………...…………………………………..5
V. 研究動機與目的……..…………………………………..…………………7
二、材料與方法………………………….…………..……….………………………8
I. 實驗材料…………..……………………………….…………………………8
II. 實驗方法…………….………………………………..…………………….8
三、實驗結果………………………………………….……….……………………12
I. 前脂肪細胞分化方式的建立……………………..………………………12
II. 綠茶兒茶素EGCG對於脂肪細胞分化的影響…..….……..………….....12
III. 綠茶兒茶素EGCG在分化過程中對各FoxO基因表現量的影響………13
IV. 不同濃度的EGCG在分化過程中對ATF-3 mRNA表現量的影響…......…15
四、討論………………………………………………….………………………..…16
I. 新的分化劑濃度可使細胞進入分化………….……………….………….16
II. 分化過程中EGCG對脂肪細胞的影響…………………………….…….16
III. EGCG在分化過程中對各FoxO mRNA表現量變化的影響…………….17
IV. EGCG在分化過程中對ATF3 mRNA表現量變化的影…….…………...19
V. 未來可能進行的實驗……………………………………….….…………19
五、結論………………………………………………………………...………………21
六、參考文獻……………………………………………………….………………...22
七、附表…………………………………...…………………….……………………29
八、附圖………………………………………...…………….………………………31
九、附錄……………………………………………...…….…………………………40

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指導教授

高永旭(Yung-Hsi Kao)

審核日期

2017-1-25

推文