控制細胞培養基板上的微米圖案與奈米結構經由修飾細胞表觀基因來達成細胞型態的控制

以作者查詢圖書館館藏

、以作者查詢臺灣博碩士

、以作者查詢全國書目

、勘誤回報

、線上人數：81

、訪客IP：18.223.206.193

姓名

顏嘉良(Chia-Liang Yen) 查詢紙本館藏

畢業系所

生物物理研究所

論文名稱

控制細胞培養基板上的微米圖案與奈米結構經由修飾細胞表觀基因來達成細胞型態的控制
(Control of cell phenotype via epigenetic modification through micro-patterning and nano-structuring of cell incubation substrate)

相關論文

★ GW準粒子於Mn3O4和GaN的激發態性質計算	★ 混合物種與低溫冷凍原子團簇噴流的發展
★ 以雷射脈衝對磁性薄膜進行超快磁轉化及其動態時間解析	★ 以脈衝雷射沈積製造FeBO3薄膜
★ 共焦拉曼與螢光顯微鏡之發展及其在材料診斷上之應用	★ 以光激發黑色素來清除細胞環境中之活性氧之探討
★ 發展在電漿波導式雷射電漿波電子加速器中誘發電子注入與X 光產生之技術	★ 莫斯堡光譜儀的建造以及其應用到FeCO3薄膜的診斷
★ 發展利用另一道脈衝雷射在脈衝雷射沉積技術中成長碳薄膜的雷射同步過程進行碳薄膜晶向之控制	★ 研究以雷射進行基板之前置處理來達到控制脈衝雷射沉積的矽鍺量子點的尺寸分布的可行性
★ 以超短脈衝雷射沉積技術製作鍺/矽薄膜之研究	★ 一百兆瓦雷射系統之建造與在結構化電漿波導之應用
★ 以基質輔助脈衝雷射蒸鍍法製備聚3-己基噻酚/(6,6)-苯基-C61-丁酸甲酯有機太陽能電池	★ 黑色素的奈米化大幅提升其用在保護細胞對抗活性氧壓力的效用
★ 藥物劑量與復原時間影響光動力療法疫苗之功效的系統性研究	★ 光控制實用的材料製程在PEM燃料電池及光電元件上的應用

檔案

[Endnote RIS 格式]

[Bibtex 格式]

[相關文章]

[文章引用]

[完整記錄]

[館藏目錄]

[檢視]

[下載]

本電子論文使用權限為同意立即開放。
已達開放權限電子全文僅授權使用者為學術研究之目的，進行個人非營利性質之檢索、閱讀、列印。
請遵守中華民國著作權法之相關規定，切勿任意重製、散佈、改作、轉貼、播送，以免觸法。

摘要(中)

在生物體的形態產生過程中，全功能性的幹細胞是藉由啟動某些基因和抑制某些基因的表現，來達成轉化為各種多功能性的細胞系，再進一步轉變為各種完全分化的細胞。但決定細胞分化類型與生物體內各種細胞的空間組織的機制，到目前為止仍未被釐清。
另一方面，細胞的行為不僅可以藉由生物化學信號來調控，也可以藉由生物物理信號來控制，尤其是來自細胞外基質的信號。眾所皆知細胞外基質的最主要組成成分是膠原蛋白微纖維束，它所具備的分子識別和結構特徵可能在控制例如分化等細胞行為和生物體形態生成上扮演關鍵的角色。尤其是，過去已有研究發現，膠原蛋白微纖維束的特殊 67 奈米周期橫紋結構，對於細胞在膠原蛋白基板上的型態與遷移方向，扮演著決定性的控制因素。
有鑑於上述這些研究的發現，我們認為可能可以利用有 67 奈米周期橫紋結構且具特定微米圖案的膠原蛋白基板來控制細胞內動蛋白纖維束的空間分佈，進而以機械作用去對細胞核內染色質的組蛋白的密度進行空間分佈上的調節，以達到對細胞表現型的控制。我們認為這可能是生物體內控制在形態生成與及腫瘤生成中細胞類型轉化的機制。而我們的研究成果顯示出利用膠原蛋白纖維束可以使幹細胞分化成神經細胞，然而有67奈米周期橫紋結構的膠原蛋白纖維束更可以提高其分化的能力。

摘要(英)

It is known that during morphogenesis, totipotent stem cells become the various pluripotent cell lines of the embryo, which in turn become fully differentiated cells, by activating some genes while inhibiting the expression of others. However, the mechanisms underlying the cell type commitment and the cells’ spatial organization in an organism are still largely unknown.
On the other hand, cellular behaviors can be modulated not only by biochemical signals but also by biophysical cues, especially those from extracellular matrix (ECM). It is well known that cell ECM is actually made of most importantly collagen microfibrils, which possess specific molecular identity and structural characteristics that may be crucial for controlling cell behaviors such as cell phenotype and coordinating organism morphogenesis. In particular, it has been reported that the characteristic 67-nm-period D-banding of collagen.
Microfibrils is required in order to observe the elongation and motional directionality of cells on a collagen substrate.
Based on these observations, we hypothesize that the cell phenotype could be controlled by spatial modulation of the chromatin in the cell nucleus through mechanical action from actin filament bundles, which in turn could be controlled by integrin clustering and activation triggered by a micropatterned collagen substrate/scaffold with D-banding. We propose that this could be what dominate cell type commitment in morphogenesis and tumorigenesis. Our result indicated that collagen microfibril could make stem cell differentiate into neuron cell, moreover, collagen microfibril with 67 nm D-banding could enhance the ability of stem cell differentiate into neuron cell.

關鍵字(中)

★ 膠原蛋白纖維束
★ 間充質幹細胞
★ 神經細胞
★ 67奈米周期橫紋結構
★ 細胞分化

關鍵字(英)

★ Collagen microfibril
★ Mesenchymal stem cell
★ Neuron cell
★ 67 nm D-banding
★ Cell differentiation

論文目次

目錄
中文提要 I
ABSTRACT II
誌謝 III
目錄 IV
圖目錄 V
表目錄 VI
一、緒論 1
1.1 組織再生工程 1
1.2 間充質幹細胞分化成神經細胞化學方法 2
1.4 控制細胞貼附大小來改變細胞分化的研究 4
1.5 膠原蛋白束之結構控制細胞行為的研究 5
二、材料與方法 7
2.1 MICRO-CONTACT PRINTING FOR FABRICATING MICROFIBRIL ISLAND ON MICA SUBSTRATE 7
2.2 印章與膠原蛋白薄膜結構表徵之量測 7
2.3 印章之製作 9
2.4 成長膠原蛋白薄膜 10
2.4.1 AFM與SEM量測 11
2.5 MSC細胞培養 11
2.6 刺激幹細胞分化成神經細胞的培養 12
2.7 神經細胞的辨識及分化比例量測(FLOW CYTOMETRY) 13
3.1 印章的製作成果 14
3.2 PDMS印章 15
3.3 膠原蛋白薄膜 16
3.4 神經細胞分化 19
四、結論與未來展望 25
4.1 結論 25
4.2 未來展望 25
參考文獻 26

參考文獻

[1] Elisabetta Ada Cavalcanti-Adam, Tova Volberg, Alexandre Micoulet, Horst Kessler, Benjamin Geiger and Joachim Pius Spatz, “Cell Spreading and Focal Adhesion Dynamics Are Regulated by Spacing of Integrin Ligands”, Biophysical Journal, Vol.92, pp2964-2974, April 2007.
[2] Deok-Ho Kim, Hyojin Lee, Young Kwang Lee, Jwa-Min Nam and Andre Levchenko, “Biomimetic Nanopatterns as Enabling Tools for Analysis and Control of Live Cells”, Advanced materials, Vol.22, Issue 41, pp4551–4566, August 2010.
[3] Lingling Hou, Hua Cao, Dongmei Wang, Guorong Wei, Cixian Bai, Yong Zhang and Xuetao Pei, “Induction of Umbilical Cord Blood Mesenchymal Stem Cells into Neuron-Like Cells In Vitro”, International Journal of Hemotology, Vol.78, pp256-261, October 2003.
[4] Evelyn K.F. Yim, Stella W. Pang and Kam W. Leong, “Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage”, Experimental cell research, Vol.313, Issue 9, pp1820-1829, May 2007.
[5] Wei Jin, Yao-peng Xu, An-Huai Yang and Y.Q. Xing , “In vitro induction and differentiation of umbilical cord mesenchymal stem cells into neuron-like cells by all-trans retinoic acid”, Int J Ophthalmol, Vol.8(2), pp250-256, April 2015.
[6] Wee Wen Leow and Wonmuk Hwang, “Epitaxially Guided Assembly of Collagen Layers on Mica Surfaces”, Langmuir, Vol.27, pp10907–10913, July 2011.
[7] David A. Cisneros, Jens Friedrichs, Anna Taubenberger, Clemens M. Franz Dr and Daniel J. Muller Prof, “Creating Ultrathin Nanoscopic Collagen Matrices For Biological And Biotechnological Applications”, Small, Vol.3, Issue 6, pp956-963, June 2007.
[8] E. Martinez, E. Engel, J.A. Planell, J. Samitier, “Effects of arti?cial micro- and nano-structured surfaces on cell behavior”, Annals of Anatomy - Anatomischer Anzeiger, Vol.191, Issue 1, pp126-135, January 2009.
[9] Christopher J. Bettinger Dr., Robert Langer Prof. and Jeffrey T. Borenstein Dr., “Engineering Substrate Topography at the Micro- and Nanoscale to Control Cell Function”, Angewandte Chemie International Edition, Vol.48, Issue 30, pp5406-5415, July 2009.
[10] Deok-Ho Kim, Paolo P. Provenzano, Chris L. Smith and Andre Levchenko, “Matrix nanotopography as a regulator of cell function”, Journal of Cell Biology, Vol.197, Issue 3, pp351-360, April 2012.
[11] Junsang Yoo, Myungkyung Noh, Hongnam Kim, Noo Li Jeon, Byung-Soo Kim and Jongpil Kim, “Nanogrooved substrate promotes direct lineage reprogramming of ?broblasts to functional induced dopaminergic neurons”, Biomaterials, Vol.45, pp36-45, March 2015.
[12] Farshid Guilak, Daniel M. Cohen, Bradley T. Estes, Jeffrey M. Gimble, Wolfgang Liedtke and Christopher S. Chen, “Control of Stem Cell Fate by Physical Interactions with the Extracellular Matrix”, Cell Stem Cell, Vol.5, Issue 1, pp17-26, July 2009.
[13] Guoping Chen, “Poly(Vinyl Alcohol)-Micropatterned Surfaces for Manipulation of Mesenchymal Stem Cell Functions”, Methods in Cell Biology, Micropatterning in Cell Biology Part A, Vol.119, chapter 2, pp.17-33, 2014.
[14] Sen Hou, Xin-Xin Li, Xiao-Yu Li, Xi-Zeng Feng, Li Guan, Yan-Lian Yang and Chen Wang, “Patterning of 293T fibroblasts on a mica surface”, Analytical and Bioanalytical Chemistry, Vol.394, Issue 8, pp2111–2117, August 2009.
[15] Fengzhi Jiang, Khaled Khairy, Kate Poole, Jonathon Howard and Daniel J. Muller, “Creating Nanoscopic Collagen Matrices Using Atomic Force Microscopy”, Microscopy Research and Technique, Vol.64, Issue 5-6, pp435-440, August 2004.
[16] Fengzhi Jiang, Heinrich Horber, Jonathon Howard and Daniel J. Muller, “Assembly of collagen into microribbons: e?ects of pH and electrolytes”, Journal of Structural Biology, Vol.148, Issue 3, pp268-278, December 2004.
[17] Kate Poole, Khaled Khairy, Jens Friedrichs, Clemens Franz, David A. Cisneros, Jonathon Howard and Daniel Mueller, “Molecular-scale Topographic Cues Induce the Orientation and Directional Movement of Fibroblasts on Two-dimensional Collagen Surfaces”, Journal of Molecular Biology, Vol.349, Issue 2, pp380-386, June 2005.
[18] Sami Alom Ruiz and Christopher S. Chen, “Microcontact printing: A tool to pattern”, Soft Matter, Vol.3 (2), pp168-177, September 2007.
[19] Likai Wang, Xizeng Feng, Sen Hou, Qilin Chan and Ming Qin, “Microcontact printing of multiproteins on the modified mica substrate and study of immunoassays”, Surface and Interface Analysis, Vol.38, Issue 1, p44-50, January 2006.
[20] Ravi A. Desai, Natalia M. Rodriguez and Christopher S. Chen, “"Stamp-off" to micropattern sparse, multicomponent features.”, Methods in Cell Biology, Volume 119,pp3-16, May 2014.
[21] Matthew J. Dalby, Nikolaj Gadegaard, Rahul Tare, Abhay Andar, Mathis O. Riehle, Pawel Herzyk, Chris D. W. Wilkinson and Richard O. C. Oreffo, “The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder”, Nature Materials, Vol.6, pp997-1003, September 2007.
[22] Ravi A. Desai, Mohammed K. Khan, Smitha B. Gopal and Christopher S. Chen, “Subcellular spatial segregation of integrin subtypes by patterned multicomponent surfaces”, Integrative Biology, Vol.3, pp560-567, February 2011

指導教授

陳賜原(Szu-yuan Chen)

審核日期

2017-1-16

推文