探討培養溫度對巴西蘑菇的常溫/高溫菌株生產β-D-glucan之影響

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葉怡芬(Yi-fen Yeh) 查詢紙本館藏

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化學工程與材料工程學系

論文名稱

探討培養溫度對巴西蘑菇的常溫/高溫菌株生產β-D-glucan之影響

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摘要(中)

食藥用菇的應用在東方文化已有幾千多年的歷史，自古以來在中藥典中即作為藥物以及菜餚的應用。巴西蘑菇學名Agaricus blazei Murill，又名巴西洋菇或姬松茸等，原產於中南美洲的巴西、祕魯地區，主要分布於海岸地帶的草原以及巴西東南部聖保羅郊外的Piedade山區。許多研究已經證實巴西蘑菇對於人體免疫系統具有許多益處，除了有預防疾病、增強免疫、抗氧化外，更有防癌抗腫瘤及調節免疫等功效。近年來巴西蘑菇備受矚目，主要因為其富含許多天然的生物活性物質，例如多醣體、beta-D-glucan 以及ergosterol等，其中又以多醣體相關文獻最多，因為多醣體具備抗腫瘤能力。
在此研究中，主要將巴西蘑菇之常溫菌株以及高溫菌株分別在不同培養溫度下進行試驗，培養溫度範圍為20℃-40℃，每5℃為區間共5個培養溫度，並分別探討其菌體生長、多醣體生成以及β-(1,3)-D glucan含量，並比較兩株巴西蘑菇菌株間的差異，綜合以上實驗結果，找到最適菌體生長以及最適β-(1,3)-D glucan生成之溫度，再利用兩階段操作(two-stage operation)方式來增加常溫菇以及高溫菇菌體生長和提高多醣體內所含之β-(1,3)-D glucan含量。

摘要(英)

In oriental culture, mushrooms has been used as health foods and home remedies for thousands of years. In several edible mushrooms, Agaricus blazei Murill was considered one of the most important edible and medicinal mushrooms native from Brazei. We also called it Agaricus brasiliensis. Many studies had proved that the Agaricus blazei Murill have strong immunomodulatory and health care functions, such as immune-enhancement, oxidation resistance, lower blood lipids et al. Recently, Agaricus blazei Murill is fascinating natural source for obtaining bioactive materials such as polysaccharides, beta-D-glucan and ergosterol. Among these chemical components, its polysaccharides with antitumor activity have attracted most attention.
Therefore, the yields and functionality of bioactive properties produced by liquid fermentation are highly depend on the culture conditions and environment parameters, such as pH, medium compositions, culture temperature and aeration rate et al.
Culture temperature is the one of the most important parameters influencing the production of bioactive properties and the cell growth. So, in my research I choose two different fruiting time of Agaricus blazei Murill to study on the effect of culture temperature ranging from 20℃ to 40℃ on cell growth, production of bioactive polysaccharides and beta-D-glucan. Agaricus blazei Murill were evaluated via five culture temperatures in step of 5℃ in a liquid fermentation, in order to optimize liquid fermentation condition of Agaricus blazei Murill for highest amount of biomass, bioactive polysaccharides and beta-D-glucan production and to compare what different between two strains of Agaricus blazei Murill. According to the results, use the two-stage temperature to increase the production of beta-D-glucan content.

關鍵字(中)

★ 巴西蘑菇
★ 液態發酵

關鍵字(英)

論文目次

摘要i
Abstract ii
目錄iii
圖目錄vii
表目錄 x
第一章、緒論 - 1 -
1-1 研究背景 - 1 -
1-2 研究目的 - 3 -
第二章、文獻回顧 - 5 -
2-1 人體免疫系統及抗腫瘤機制 - 5 -
2-1-1 免疫系統 - 5 -
2-2 菇類介紹 - 5 -
2-2-1 菇類活性物質 - 7 -
2-2-2 菇類多醣體 - 8 -
2-3 巴西蘑菇(Agaricus blazei Murill) - 10 -
2-3-1 巴西蘑菇化學成分 - 13 -
2-3-2 巴西蘑菇抗氧化物質 - 15 -
2-3-3 巴西蘑菇藥理研究 - 15 -
2-3-3 巴西蘑菇多醣體 - 19 -
2-3-4 巴西蘑菇多醣體結構 - 20 -
2-4 深層液態發酵培養 - 21 -
2-5 影響發酵之因素 - 22 -
2-5-1 培養機組成 - 22 -
2-5-2 培養條件 - 26 -
第三章材料與方法 - 33 -
3-1 實驗材料 - 33 -
3-1-1 實驗菌株 - 33 -
3-1-2 巴西蘑菇培養基 - 34 -
3-1-3 還原糖分析 - 34 -
3-1-4 多糖分析 - 34 -
3-1-5 β-(1,3)-D glucan 螢光分析 - 35 -
3-2 實驗儀器 - 35 -
3-3 巴西蘑菇搖瓶實驗 - 36 -
3-3-1 巴西蘑菇菌株保存 - 36 -
3-3-2 培養基組成 - 36 -
3-3-3 實驗方法 - 38 -
3-4 研究設計 - 42 -
3-5 分析方法 - 43 -
3-5-1 菌絲體乾重分析 - 43 -
3-5-2 葡萄糖殘量分析 - 43 -
3-5-3 多醣體分析 - 45 -
3-5-4 β-(1,3)-D glucan 濃度測定 - 46 -
第四章、結果與討論 - 49 -
4-1 菌絲體於不同固態培養基之型態 - 49 -
4-2 培養溫度對於常溫菇與高溫菇菌重之影響 - 51 -
4-3 發酵液 pH 值之變化 - 54 -
4-4 溫度對於發酵液中總多醣體含量之影響 - 55 -
4-5 溫度對於 β-(1,3)-D glucan 之影響 - 57 -
4-5-1 總 β-(1,3)-D glucan 含量 - 57 -
4-5-2 相對 β-(1,3)-D glucan - 59 -
4-6 提高 β-(1,3)-D glucan 發酵測驗 - 60 -
第五章、結論 - 67 -
第六章、參考文獻 - 68 -

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指導教授

徐敬衡

審核日期

2017-8-10

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