| 摘要(英) |
We investigate two-component membranes composed of POPE and POPC, and three-component membranes composed of POPE, POPC and sterol. The phase behavior of POPC/POPE membranes was studied as a function of POPE concentration and temperature by 2H-NMR and DSC. For 2H-NMR measurements, POPC and POPE were deuterium labeled alternatively such that the behavior of both POPC and POPE can be observed. We present the first temperature-composition diagram for POPE concentration range from 0 to 60 mol%. Coexistence of the gel and lc phases is observed over a wide temperature and composition range. It is found that POPC and POPE do not mix well in 1:1 POPC/POPE below17℃, but mix well above 17℃.
To investigate the properties of POPC/POPE membranes in the presence of sterol, 1:1:1 POPC/POPE/ergosterol and 1:1:1 POPC/POPE/cholesterol were studied as a function of temperature by 2H-NMR, with POPC and POPE deuterium labeled alternatively. We show that the presence of ergosterol (or cholesterol) disorders gel-phase POPC/POPE (1:1) membranes, whereas orders lc-phase POPC /POPE (1:1) membranes. The effect of ergosterol is weaker than cholesterol. Furthermore, the presence of ergosterol (or cholesterol) modulates the mixing properties of POPC/POPE membranes. POPC and POPE mix well in some temperature range, where they are poorly mixed in the absence of sterol. The enhancement of mixing of of POPC/POPE membranes by ergosterol is observed in a temperature range narrower than that by cholesterol. |
| 參考文獻 |
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