基於PEG的益生元影響皮膚細菌和皮膚電的發酵

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姓名

艾德(Ider Sarantuya) 查詢紙本館藏

畢業系所

生醫科學與工程學系

論文名稱

基於PEG的益生元影響皮膚細菌和皮膚電的發酵
(PEG-based Prebiotic Affects Fermentation of Skin Bacteria and Dermal Electricity)

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摘要(中)

摘要
皮膚電導是皮膚電導率的測量。表皮葡萄球菌（表皮葡萄球菌），是一種革蘭氏陽性細菌，屬於金黃色葡萄球菌的40多種物種之一，可以將甘油代謝成SCFAs並產生電能。因此，我們假設細菌發酵影響皮膚電導。在該研究中，PEG-150二硬脂酸酯（PDS）用作碳源以誘導表皮葡萄球菌的發酵並產生電。在體外實驗中，電可以顯著抑制自由基，這是通過產生脂質過氧化的主要終產物之一4-羥基壬烯（HNE）來測量的。具有表皮葡萄球菌的PDS引發小鼠皮膚中的顯著電力升高。初步結果表明，表皮葡萄球菌PDS發酵產生的電子抑制了應激誘導的小鼠毛髮生長抑制。

摘要(英)

Abstract
Skin conductance is the measurement of the electrical conductivity of the skin. Staphylococcus epidermidis (S. epidermidis), is a Gram-positive bacterium, and one of over 40 species belonging to the genus Staphylococcus which can metabolize glycerol to SCFAs and produce electricity. We thus hypothesize that bacterial fermentation affects the skin conductance. In this study, PEG-150 Distearate (PDS), was used as a carbon source to induce fermentation of S. epidermidis and generated electricity. In vitro experiment, electricity can significantly suppress the free radicals which were measured by the production of 4-Hydroxynonenal (HNE), one of the major end products of lipid peroxidation. PDS with S. epidermidis, triggered a significant elevation of electricity in mouse skin. Preliminary results indicated that electron produced by PDS fermentation of S. epidermidis inhibited the stress-induced suppression of hair growth in mice.

關鍵字(中)

★ 自由基
★ 電
★ 發酵
★ IL-6
★ PDS 2％
★ 表皮葡萄球菌

關鍵字(英)

★ Staphylococcus epidermidis
★ Fermentation
★ Electricity
★ Free radicals
★ IL-6
★ PDS 2%

論文目次

Table of content
1. Introduction………………………………...……...………………………….1
1.1 Skin……………………………………………….………………………1
1.2 Hair Growth…………………...…………………………………………2
1.3 Hair follicle and mechanism of the hair growth………………...……….2
1.4 Fermentation of Bacteria………...………………………………………3
1.5 Bacterium…………………………………...………………...…………5
1.6 PDS…….………………………………………………………………...6
1.7 Free Radicals………………………………...…………………………..7
1.7.1 Oxidation Factors……………………………………………...7
1.7.2 Disease Risks………………………………………….………8
1.7.3 Important Antioxidants……………………………….……….8
1.8 IL-6……………………………………...……………………………….9
1.9 Skin Patch………………………………………………………………10
1.10 Stressor………………………………………………………………11
2. Material and Methods……………………………………...………………..12
2.1 Materials………………………………………………………………...12
2.1.1 Apparatus or Instruments……………………………………...12
2.1.2 Reagents……………………………………………………….13
2.2 Methods…………………………………………………………………14
2.2.1 The Growth of Bacteria…………………...…………………..14
2.2.2 OD measurement, Decrease the Bacterial OD………………..14
2.2.3 Medium Preparation…………………………………………...14
2.2.4 Animal Experiment……………………………………………15
3. Result..……………………………………………………………………….16
3.1 0.72% Mushroom powder fermentation of S. staphylococcus……..……16
3.2 Regular Rice powder (0.72%) fermentation of S. staphylococcus….…18
3.3 0.02% of PDS fermentation of Staphylococcus epidermidis and voltage measurement on mouse dorsal back skin………...……………………..19
3.4 Time Point and IL-6 result……………………………………………...21
3.5 Western blotting and result…………………………………………...…23
4. Discussion and Conclusion………………………...………………………..24
5. References……………………………………………..……………………25

參考文獻

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指導教授

黃俊銘(Chun-Ming Huang)

審核日期

2019-7-12

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