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姓名	陳柏霖(Po-Ling Chen)  查詢紙本館藏	畢業系所	光電科學與工程學系
論文名稱	雙光子螢光超光譜顯微術於提琴材料之研究 (The research of violin and cello material using two-photon fluorescence hyperspectral microscopy)
相關論文	★ 非反掃描式平行接收之雙光子螢光超光譜顯微術 ★ 以二次通過成像量測架構及降低誤差迭代演算法重建人眼之點擴散函數 ★ LASER光源暨LED在老鼠毛生長的低能量光治療比較分析 ★ 應用線狀結構照明提升雙光子顯微鏡解析度 ★ 以同調結構照明顯微術進行散射樣本解析度之提升 ★ 掃描式二倍頻結構照明顯微術 ★ 小貓自泵相位共軛鏡於數位光學相位共軛與時間微分之研究 ★ 鏡像輔助斷層掃描相位顯微鏡 ★ 以數位全像術重建多波長環狀光束之研究 ★ 相位共軛反射鏡用於散射介質中光學聚焦之研究 ★ 雙光子螢光超光譜顯微術於多螢光生物樣本之研究 ★ 倍頻非螢光基態耗損超解析之顯微成像方法 ★ 葉綠素雙光子螢光超光譜影像於光合作用研究之應用 ★ 雙光子掃描結構照明顯微術 ★ 微投影光學切片超光譜顯微術 ★ 使用結構照明顯微術觀察活體小鼠毛囊生長週期之變化
檔案	[Endnote RIS 格式]    [Bibtex 格式]    [相關文章]   [文章引用]   [完整記錄]   [館藏目錄]   [檢視]  [下載] 本電子論文使用權限為同意立即開放。 已達開放權限電子全文僅授權使用者為學術研究之目的，進行個人非營利性質之檢索、閱讀、列印。 請遵守中華民國著作權法之相關規定，切勿任意重製、散佈、改作、轉貼、播送，以免觸法。
摘要(中)	許多小提琴與大提琴，都是選用雲杉來當作面板，各種不同的音色與音質，都會因為選用不同材質的木頭而有所差異，珍藏在博物館的古老小提琴與大提琴，有些已經有兩百年的歷史，令人好奇當年的製作工法與現今有何差異，科學家針對提琴的塗漆與化學分子結構變化進行研究，目前與提琴有關的研究發現古代名琴的背板楓木確實有做過化學處理且泡過礦物質含有金屬元素成分，且確定提琴纖維素的結晶性完整，但面板雲杉至今還沒有完整的研究結果。螢光光譜作為分析方法擁有的優點為高靈敏度、快速性與低成本，且能夠對於樣本進行低破壞性的測量，成為一個非常有吸引力的分析方法。 本研究使用掃描式雙光子螢光超光譜顯微術來取得古代名琴雲杉與現代雲杉的超光譜影像，超光譜影像具有一維光譜資訊及二維空間資訊，除了能分析樣本的平均光譜外，亦能進一步了解管胞與壁孔之間光譜資訊的差異並進行分析，本研究利用高斯函數擬合找出螢光光譜基底，希望藉由螢光光譜基底來解釋現代雲杉與古代名琴雲杉螢光光譜之間的差異，並搭配線性分離法準確地將二倍頻訊號自光譜中分離出來，利用二倍頻訊號來分析纖維素在木頭裡的分布情況，比較古代名琴與現代雲杉纖維素的強度分布，並推測經過三百年後古代名琴雲杉中的纖維素產生了何種變化。
摘要(英)	Many violins and cellos use spruce as a panel. A variety of different sound quality will change due to the choice of wood of different materials. The ancient violin and cello which are treasured in the museum for two hundred years, making people wonder how the previous production methods differ from today. Scientists study the painting and chemical molecular structure changes of the violin and cello. It is found that the maple of the ancient violin and cello has indeed been chemically treated, and the maple of the ancient violin and cello containing metal elements, and the crystallinity of the cellulose of the violin is determined to be complete. But the spruce hasn’t related research yet. Fluorescence spectroscopy has the advantages of high sensitivity, rapidity and low cost as analytical methods, and enables low destructive measurement of samples. A line scanning two-photon fluorescence hyperspectral microscopy is used to obtain hyperspectral images of ancient spruce and modern spruce. Hyperspectral image has one-dimensional spectral information and two-dimensional spatial information. In this research, the Gaussian function fitting is used to find the fluorescence spectral base. It hopes to explain the difference between the fluorescence spectrum of modern spruce and ancient spruce is explained by the fluorescence spectrum base, and the fluorescence spectrum and the second-harmonic generation are accurately separated by linear unmixing. Using the second-harmonic generation to analyze the distribution of cellulose in wood, and comparing the intensity distribution of ancient spruce cellulose and modern spruce cellulose, try to speculate on how the ancient spruce changed after three hundred years.
關鍵字(中)	★ 雙光子螢光超光譜顯微術 ★ 提琴材料 ★ 史特拉底瓦里 ★ 螢光光譜 ★ 二倍頻 ★ 線性分離法	關鍵字(英)
論文目次	中文摘要..........................I Abstract.........................II 目錄.............................III 圖目錄............................V 表目錄............................VII 第一章 緒論.........................1 1.1 文獻回顧與探討.................1 1.2 研究動機與目的.................7 1.3 論文架構.......................7 第二章 實驗原理與分析法.............8 2.1 木頭的結構.....................8 2.2 雙光子顯微術...................9 2.3 雙光子超光譜影像...............11 2.4 線性分離法.....................17 第三章 實驗架構與方法...............20 3.1 實驗系統架構...................20 3.2 實驗系統光譜校正................22 3.3 系統解析度分析.................23 3.3.1 光譜解析度...................23 3.3.2 空間解析度...................24 3.4 樣本備置.......................28 第四章 實驗結果與討論...............29 4.1 提琴木屑的螢光超光譜影像.........29 4.2 螢光光譜基底....................36 4.3 線性分離法之結果與討論..........41 4.3.1 螢光光譜基底之影像............41 4.3.2 二倍頻訊號之分析..............47 第五章 結論........................49 參考文獻...........................50
參考文獻	[1] L. Burckle, and H. D. Grissino-Mayer, "Stradivari, violins, tree rings, and the Maunder Minimum: a hypothesis," Dendrochronologia, Vol. 21, No. 1, pp. 41-45, 2003. [2] A. Bohlen, "Quantitative Analysis of Minor and Trace Elements in Historical Varnishes Using Total Reflection X‐Ray Fluorescence," Analytical Letters, Vol. 37, No. 3, pp. 491-498, 2004. [3] F. Caruso, S. Saverwyns, M. V. Bos, D. F. C. Martino, A.-E. Ceulemans, J. Valck, and E. Caponetti, "Micro-X-Ray Fluorescence and the Old Masters," Applied Physics A, Vol. 107, No. 1, pp. 197-202, 2012. [4] J. Topham, and D. McCormick, "FOCUS: A Dendrochronological Investigation of Stringed Instruments of the Cremonese School (1666–1757) including ‘‘The Messiah’’ violin attributed to Antonio Stradivari," Journal of Archaeological Science, Vol. 27, No. 3, pp. 183-192, 2000. [5] C. Invernizzi, A. Daveri, T. Rovetta, M. Vagnini, M. Licchelli, F. Cacciatori, and M. Malagodi, "A multi-analytical non-invasive approach to violin materials: The case of Antonio Stradivari “Hellier” (1679)," Microchemical Journal, Vol. 124, pp. 743-750, 2016. [6] J.-P. Echard, "In situ multi-element analyses by energy-dispersive X-ray fluorescence on varnishes of historical violins," Spectrochimica Acta, Vol. 59, No. 10-11, pp. 1663-1667, 2004. [7] S. Robinson, and A. H. Robinson, "Chemical Composition of Sweat," Physiological Reviews, Vol. 34, No. 2, pp. 202, 1954. [8] H. C. Tai, G. C. Li, S. J. Huang, C. R. Jhu, J. H. Chung, B. Y. Wang, C. S. Hsu, B. Brandmair, D. T. Chung, H. M. Chen, and J. C. C. Chan, "Chemical distinctions between Stradivari’s maple and modern tonewood, " PNAS, Vol. 114, No. 1, pp. 27-31, 2017. [9] M. Yokoyama, J. Gril, M. Matsuo, H. Yano, J. Sugiyama , B. Clair, S. Kubodera , T. Mistutani, M. Sakamoto, H Ozaki, M. Imamura, and S. Kawai, "Mechanical characteristics of aged Hinoki wood from Japanese historical buildings," Comptes Rendus Physique, Vol. 10, No. 7, pp. 601-611, 2009. [10] S. T. Dyer, "Wood fluorescence of indigenous south african trees," IAWA Bulletin, Vol. 9, No. 1, pp. 75-87, 1988. [11] S. T. Sum, D. L. Singleton, G. Paraskevopoulos R. S. Irwin, R. J. Barbour, and R Sutcliffe, "Laser-excited fluorescence spectra of eastern SPF wood species. An optical technique for identification and separation of wood species?," Wood Science and Technology, Vol. 25, No. 6, pp. 405-413, 1991 [12] K. K. Pandey, N. K. Upreti, and V. V. Srinivasan, "A fluorescence spectroscopic study on wood," Institute of Wood Science and Technology, Vol. 32, No. 4, pp. 309-315, 1998. [13] E. Billa, A. Pastou, B. Monties, J. Romero, and E. G. Koukios, "Multivariate chemometric analysis of the fluorescence spectra of eucalyptus wood," Industrial Crops and Products, Vol. 11, No. 2-3, pp. 187-196, 2000. [14] J. Nagyvary, J. A. DiVerdi, N. L. Owen, and H. D. Tolley, "Wood used by Stradivari and Guarneri," Nature, Vol. 444, pp. 565, 2006. [15] R. A. Parham, and R. L. Gray, "Formation and Structure of Wood," Advances in Chemistry, Vol. 207, pp. 3-56, 1984. [16] C. Loix, M. Huybrechts, J. Vangronsveld, M. Gielen, E. Keunen , and A. Cuypers, "Reciprocal Interactions between Cadmium-Induced Cell Wall Responses and Oxidative Stress in Plants," Frontiers in Plant Science, Vol. 8, pp. 1-19, 2017. [17] D. Djikanovic, A. Devecerski, G. Steinbach, J. Simonovic, B. Matovic, G. Garab, A. Kalauzi, and K. Radotic, "Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction," Wood Science and Technology, Vol. 50, No. 3, pp. 547-566, 2016. [18] https://www.sciencedirect.com/topics/materials-science/softwood [19] M. G. Mayer, "Uber elementarakte mit zwei quantensprungen," Wiley Online Library, Vol. 401, No. 3, pp. 273-294, 1930. [20] F. Helmchen, and W. Denk, "Deep tissue two-photon microscopy," Nature, Vol. 2, No. 12, pp. 932-938, 2005. [21] W. Denk, J. H. Strickler, and W. W. Webb, "Two-Photon Laser Scanning Fluorescence Microscopy," Science, Vol. 248, No. 4951, pp. 74-76, 1990. [22] H.-W. Liu, Y. Liu, P. Wang, and X.-B. Zhang, "Molecular engineering of two-photon fluorescent probes for bioimaging applications," IOP Publishing, Vol. 5, No. 1, pp.1-24, 2017. [23] C. R. Graham, " Two-photon uncaging of glutamate," Frontiers, Vol. 10, pp. 1-13, 2019. [24] G. Cox, and N. Moreno, "Second-harmonic imaging of plant polysaccharides," Journal of Biomedical Optics, Vol. 10, No. 2, pp. 024013-1-024013-6, 2005. [25] P. A. Franken, A. E. Hill, C. W. Peters, and G. Weinreich, " Generation of optical harmonics," Physical review letters, Vol. 7, No. 4, pp. 118-119, 1961. [26] S. W. Paddock, " Confocal Laser Scanning Microscopy," BioTechniques, Vol. 27, No. 5, pp.992-1004, 1999. [27] W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science, Vol. 248, pp. 73-76, 1990. [28] T. Vo-Dinh, "A hyperspectral imaging system for in vivo optical diagnostics," IEEE Engineering in Medicine and Biology Magazine, Vol. 23, pp. 40-49, 2004. [29] A. Goetz, G. Vane, J. Solomon, and B. Rock, "Imaging spectrometry for earth remote sensing," Science, Vol. 228, pp. 1147-1152, 1985. [30] W. R. Johnson, D. W. Wilson, W. Fink, M. Humayun, and G. Bearman, " Snapshot hyperspectral imaging in ophthalmology, "Journal of Biomedical Optics, Vol. 12, pp. 014036-1-14036-7, 2007. [31] G. Lu, and B. Fei, "Medical hyperspectral imaging: a review," Journal of Biomedical Optics, Vol. 19, No. 1, pp. 010901-1-010901-23, 2014. [32] Y. Uno, S. O. Prasher, R. Lacroix, P. K. Goel, Y. Karimi, A. Viau, and R. M. Patel, "Artificial neural networks to predict corn yield from Compact Airborne Spectrographic Imager data, " Computers and Electronics in Agriculture, pp. 149-161, 2005. [33] D. Wu , and D.-W. Sun, "Advanced applications of hyperspectral imaging technology for food quality and safety analysis and assessment: A review— Part I: Fundamentals," Innovative Food Science & Emerging Technologies, Vol. 19, pp. 1-28, 2013. [34] A. A. Gowena, C. P. O’Donnell, P. J. Cullenb, G. Downeyc, and J. M. Frias, "Hyperspectral imaging e an emerging process analytical tool for food quality and safety control," Trends in Food Science & Technology, Vol. 18, pp. 590-598, 2007. [35] R. M. Levenson, P. J. Cronin, and N. R. Harvey, "Spectral imaging and biomedicine: new devices, new approaches," IEEE, Vol. 31, pp. 105-111, 2002. [36] Z. Liu, J.-q. Yan, D. Zhang, and Q.-L. Li, "Automated tongue segmentation in hyperspectral images for medicine," Applied Optics, Vol. 46, pp. 8328-8334, 2007. [37] J. M. Benavides, S. Chang, S. Y. Park, and R. R. Kortum, "Multispectral digital colposcopy for in vivo detection of cervical cancer," Optics express,Vol. 11, No. 10, pp. 1223-1236, 2003. [38] S. T. Monteiro, Y. Minekawa, Y. Kosugi, T. Akazawa, and K. Oda, "Prediction of sweetness and amino acid content in soybean crops from hyperspectral imagery," ISPRS Journal of Photogrammetry & Remote Sensing, Vol. 62, pp. 2-12, 2007. [39] A. A. Gowen, M. Taghizadeh, and C. P. O’Donnell, "Identification of mushrooms subjected to freeze damage using hyperspectral imaging," Journal of Food Engineering, Vol. 93, pp. 7-12, 2009. [40] A. F. H. Goetz, "Three decades of hyperspectral remote sensing of the Earth: A personal view," Remote Sensing of Environment, Vol. 113, pp. 5-16, 2009. [41] Q. Li, X. He, Y. Wang, H. Liu, D. Xu, and F. Guo, "Review of spectral imaging technology in biomedical engineering: achievements and challenges," Journal of Biomedical Optics, Vol. 18, No. 10, pp. 100901-1-010901-28, 2013. [42] 呂喬聖，「非反掃描式平行接收之雙光子螢光超光譜顯微術」，國立中央大學，碩士論文，民國102年。 [43] S.-Y. Chen, C.-S. Lu, and C.-H. Yeh, "Non-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution," OSA,Vol. 5, No. 2, pp. 338-347, 2014. [44] M. B. Sinclair, D. M. Haaland, J. A. Timlin, and H. D. Jones, "Hyperspectral confocal microscope," Applied Optics, Vol. 45, pp. 6283-6291, 2006. [45] T. Zimmermann, "Spectral Imaging and Linear Unmixing in Light Microscopy," Microscopy Techniques, Vol. 95, pp. 245-265, 2005. [46] H. Tsurui, H. Nishimura, S. Hattori, S. Hirose, K. Okumura, and T. Shirai, "Seven-color Fluorescence Imaging of Tissue Samples Based on Fourier Spectroscopy and Singular Value Decomposition," The Journal of Histochemistry & Cytochemistry, Vol. 48, No. 5, pp. 653-662, 2000. [47] G. Peters, and J. H. Wilkinson, "The least squares problem and pseudo-inverses," The Computer Journal, Vol. 13, No. 3, pp. 309-316, 1970. [48] D. Djikanovic, A. Devecerski, G. Steinbach, J. Simonovic, B. Matovic, G. Garab, A. Kalauzi, and K. Radotic, " Comparison of macromolecular interactions in the cell," Wood Science and Technology, Vol. 52, No. 3, pp. 547-566, 2016. [49] P. J. Chantry, " Doppler Broadening in Beam Experiments," The Journal of Chemical Physics, Vol. 55, No. 6, pp. 2746-2759, 1971.
指導教授	陳思妤(Szu-Yu Chen)	審核日期	2019-8-21
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