以奈米矽質譜晶片檢測濫用藥物與輕量化質譜 儀整合之研究

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姓名

黃志穎(Chih-Ying Huan1) 查詢紙本館藏

畢業系所

機械工程學系

論文名稱

以奈米矽質譜晶片檢測濫用藥物與輕量化質譜儀整合之研究

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至系統瀏覽論文 (2025-12-21以後開放)

摘要(中)

常見的管制藥品與毒品是處於一體兩面的關係，非醫療使用目的且未經醫師開立處方而濫用管制藥品，便會使管制藥品成為毒品。而近年來越發流行的新興毒品(New Psychoactive Substance,NPS)也是類似的關係。隨著越來越多的藥物遭到濫用，常用在一線檢測的化學呈色法以及免疫層析法也不一定能有效的應對，因此尋求著能更有效的進行初步檢驗的新方法。
nSi做為一種表面輔助雷射脫附游離(Surface assisted laser desorption ionization)技術，是一種不需要加入基質且檢測時程短的質譜檢測方式，並有著快速製作、成本低，容易的整合至商用的MALDI機台等優點。本研究透過nSi在質譜檢測上的優勢，針對Mephedrone與Methylone兩種新興毒品以及一些醫療濫用藥物進行質譜檢測，同時輔以基質增強(Matrix-Enhanced)的方式，嘗試優化nSi檢測藥物的靈敏度。除此之外，也測試nSi在做為質譜基板的同時，進行超薄層層析的分離效果。
雷射脫附游離法通常是搭配飛行時間質譜儀(time-of-flight mass spectrometry, TOF-MS)使用，然而常見的商用TOF-MS通常體積高大且價格動輒百萬。因此在使用TOF進行研究的同時，我們也與國內質譜儀廠商合作，在體積更小(≒0.)的離子阱質譜儀(Quadrupole ion trap mass spectrometry)原型機上進行nSi的整合，並參與開發與測試低質量的質譜應用。

摘要(英)

Recently, the issue of drug abuse has become increasingly serious. In addition to controlled drugs, there is also the phenomenon of abuse of drugs that have not been listed, called New Psychoactive Substance(NPS). As more and more drugs are abused, the coloration or immune chromatography methods, which are commonly used in preliminary tests may not be effective. Therefore, it is necessary to find new methods that can perform preliminary inspections more effectively.
As a surface assisted laser desorption ionization technology for mass spectrometry, nSi does not require a matrix, which will cause noise in the low m/z region. And it has the advantages of rapid analysis, simple production, low cost, and easy integration into MALDI –TOF mass spectrometer. This research will use the advantages of nSi in mass spectrometry to analysis two NPS, Mephedrone and Methylone, and some medication. At the same time, the matrix-enhanced method is used to optimize the sensitivity of nSi to detect drugs. In addition, we also tested the performance of ultra-thin layer chromatography while using nSi as a mass spectrometer substrate.
Because common mass spectrometers are usually large and expensive. Therefore, we also cooperating with mass spectrometer manufacturer to integrate nSi with quadrupole ion trap mass spectrometry, which smaller than MALDI-TOF-MS. And participate in the development of low-quality mass spectrometry applications.

關鍵字(中)

★ 質譜
★ 表面輔助雷射脫附游離
★ 毒品
★ 分離
★ 分析

關鍵字(英)

★ Mass spectrometry
★ Surface-assisted laser desorption/ionization (SALDI)
★ Drug
★ Separate
★ analysis

論文目次

目錄
摘要 i
致謝 iii
目錄 iv
第一章前言 1
1.1 質譜相關技術介紹 1
1.1.1 MALDI (Matrix-assisted laser desorption/ionization) 1
1.1.2 SALDI (Surface assisted laser desorption/ionization) 2
1.1.3 DIOS 2
1.1.4 SiNWs 4
1.1.5 DIOS基板對基質的游離的影響 4
1.1.6 Ultra thin layer chromatography (UTLC) 5
1.2 質量分析器 7
1.2.1 飛行時間質譜法(Time-of-flight,TOF) 7
1.2.2 四極離子阱(Quadrupole ion trap,QIT) 9
1.3 離子偵測器 13
1.4 新興毒品與濫用藥物的流行與影響 15
第二章材料與方法 17
2-1 實驗材料與設備 17
2-1.1 實驗材料與樣本 17
2-1.2 實驗設備 18
2-2 nSi晶片製作 18
2-3 樣本清單 20
2-3-1 藥物樣本清單 20
2-3-2 尿液前處理與檢體清單 20
2-4 實驗方法 24
2-4-1 MALDI TOF/TOF質譜分析 24
2-4-2 MALDI-QIT質譜分析 25
2-4-3 UTLC-MS 26
第三章結果與討論 27
3-1 新興毒品與藥物樣品檢測 27
3-1-1 藥物樣本之nSi檢測 27
3-1-2 藥物樣本的檢測極限 38
3-1-3 尿液檢體的nSi檢測 44
3-1-4 Matrix-Enhanced nSi 性能測試 47
3-1-5 透過Matrix-Enhanced nSi 的新興毒品檢測 50
3-1-6 UTLC-nSi質譜晶片 52
3-2 MALDI-QIT 57
3-2-1 訊號判讀 57
3-2-2 MALDI-QIT質譜流程調置 59
3-2-3 nSi-QIT 68
第四章結論 72
參考文獻 74

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指導教授

曹嘉文(Chia-Wen Tsao)

審核日期

2020-12-7

推文