博碩士論文 108821601 詳細資訊




以作者查詢圖書館館藏 以作者查詢臺灣博碩士 以作者查詢全國書目 勘誤回報 、線上人數:44 、訪客IP:18.220.13.70
姓名 孟里凡(ivan mambaul munir)  查詢紙本館藏   畢業系所 生命科學系
論文名稱 Genetic Transformation of The Green Algae Micractinium tetrahymenae by Agrobacterium Mediated transformation
(Genetic Transformation of The Green Algae Micractinium tetrahymenae by Agrobacterium Mediated transformation)
檔案 [Endnote RIS 格式]    [Bibtex 格式]    [相關文章]   [文章引用]   [完整記錄]   [館藏目錄]   [檢視]  [下載]
  1. 本電子論文使用權限為同意立即開放。
  2. 已達開放權限電子全文僅授權使用者為學術研究之目的,進行個人非營利性質之檢索、閱讀、列印。
  3. 請遵守中華民國著作權法之相關規定,切勿任意重製、散佈、改作、轉貼、播送,以免觸法。

摘要(中) 綠藻被視為是一個生產生物質的理想平台,而生物質可以運用在人類及動物的食物,以及進一步生產生質能源。基因改造已經變成是一項可以加速生物質生產之強而有力的工具,並且可以降低生產時的花費,使得整體生產更加經濟實惠。然而,一直以來綠藻的基因改造總是面對許多困難,例如:低的轉型成功率、不同數目的轉基因拷貝數以及不穩定的基因表現。在許多轉殖外來基因的轉型方法中,農桿菌 (Agrobacterium sp.) 調控的轉型方法最為常用。雖然這項技術在一些藻類中可以作用,包括: Chlamydomonas reinhardtii, Haematococcus pluvialis 以及 Chorella vulgaris,但是轉型的效率會因不同的物種而有所差異。Micractinium tetrahymenae,一種會與四膜蟲形成內共生的綠藻,具有作為生產平台很好的前瞻,因為其具有產生大量的生物質的可能性。在這項研究中,我們首次成功的將帶有帶有潮黴素以及綠螢光蛋白T-DNA的Agrobacterium tumefaciens,轉型Micractinium tetrahymenae 的基因體中。轉型的方法是基於潮黴素抗藥性的表現型,我們也更進一步地提供PCR (聚合酶連鎖反應) 以及綠色螢光強度的差異作為轉型成功的證據。
摘要(英) The green algae are considered as an ideal platform for biomass production which can be used for human food, animal feed and biofuels. Genetic modification has become a robust approach to speed up the development of biomass productions and reduce the production cost, making the production economically feasible. The main issues for the genetic modification of green algae are the low transformation efficiency, varied copy numbers of transgene integration and instability of gene expression. Among the transformation methods for delivering exogenous DNA, Agrobacterium-mediated transformation is the most commonly used. Although this technique has been shown to work with a few algae, including Chlamydomonas reinhardtii, Haematococcus pluvialis and Chorella vulgaris, its working efficiency varies depending on the species. The green algae, Micractinium tetrahymenae, has a great prospect as a production platform due to its potential ability to produce a large amount of biomass and the first green algae representative of endosymbiosis found in Tetrahymena utriculariae. In this study, we report the first successful transfer of Agrobacterium tumefaciens T-DNA carrying the genes coding for a drug (hygromycin) resistant marker and green fluorescent protein (GFP) to the nuclear genome of Micractinium tetrahymenae. The transformation protocol was based on the hygromycin resistance phenotype. We further provide transformation evidence by PCR and measuring the green fluorescence intensity in the transformed cells.
關鍵字(中) ★ Agrobacterium Mediated Transformation
★ The Green algae
★ Genetic modification
★ Micractinium tetrahymenae
★ Biomass
關鍵字(英) ★ Agrobacterium Mediated Transformation
★ The Green algae
★ Genetic modification
★ Micractinium tetrahymenae
★ Biomass
論文目次 Table of contents
摘要 ........................................................................................................................................................... i
Abstract ................................................................................................................................................... ii
Acknowledgments ................................................................................................................................ iii
Table of contents .................................................................................................................................. iv
List of tables .......................................................................................................................................... vi
List of figures ........................................................................................................................................ vii
CHAPTER 1. INTRODUCTION ...........................................................................................................1
1.1. Background...............................................................................................................................1
1.2. Objectives .................................................................................................................................3
1.3. Research description ................................................................................................................3
1.4. Thesis organization ...................................................................................................................3
CHAPTER 2. LITERATURE REVIEW ...............................................................................................5
2.1. Introduction of The green algae Micractinium tetrahymenae .....................................................5
2.2 Environmental requirements of the green algae Micractinium tetrahymenae (light, Heat, medium, Nutrition) ..............................................................................................................................8
2.2.1 Algae culturing/medium..........................................................................................................8
2.2.2 Light .........................................................................................................................................8
2.2.3 Temperature ...........................................................................................................................9
2.2.4. Nutritional factors ............................................................................................................... 10
2.2.5. pH and salinity ..................................................................................................................... 12
2.3. Gene transfer and genetic transformation/genetic engineering ............................................... 13
2.5. Agrobacterium tumefaciens-mediated gene transfer to the green algae ................................. 14
2.5.1. Agrobacterium tumefacients .............................................................................................. 14
2.5.2. The transfer of T-DNA from Agrobacterium tumefaciens into green algae ....................... 14
CHAPTER 3. MATERIAL AND METHODS .................................................................................... 17
3.1 Micrac tinium tetrahemynae and culture conditions .................................................................. 17
3.2. Agrobacterium strain and vectors .............................................................................................. 17
3.3 Antibiotic sensitivity test ............................................................................................................. 17
3.4 Plasmid construct and bacterial strains ...................................................................................... 18
3.5. Bacteria transformation ............................................................................................................. 18
3.6. Transformation of Micractinium tetrahemynae ........................................................................ 18
3.7. Optimisation of transformation variable ................................................................................... 20
3.8. Selection of transformant lines .................................................................................................. 20
3.9. Detection of reporter gene expression ...................................................................................... 20
3.10. PCR analysis of transformants .................................................................................................. 21
v
3.11. Detection of Agrobacterium tumefaciens contamination in the transformants ..................... 22
3.12. Statistical analysis of T-DNA integration and transgene expression ........................................ 22
CHAPTER 4. RESULTS ......................................................................................................................... 23
4.1. Growth rate of Micractinium tetrahymena ............................................................................... 23
4.2. Optimization of the selection conditions for Micractinium tetrahymena ................................. 24
4.3 Establishment of Agrobacterium-mediated transformation method for Micractinium tetrahymena ...................................................................................................................................... 26
4.4. Construction of plasmid ............................................................................................................. 28
4.5. Confirmation of transformation into Micractinium tetrahymena genome ............................... 29
4.6. Expression analyses of mGFP gene ............................................................................................ 32
CHAPTER 5. DISCUSSION ............................................................................................................. 35
CHAPTER 6. GENERAL CONCLUSION ....................................................................................... 39
References .......................................................................................................................................... 40
參考文獻 Almerei Ayman. 2016. “Agrobacterium-Mediated Transformation of Syrian Maize with Anti-Stress Genes.”
Anila, N., Arun Chandrashekar, G. A. Ravishankar, and R. Sarada. 2011. “Establishment of Agrobacterium Tumefaciens-Mediated Genetic Transformation in Dunaliella Bardawil.” European Journal of Phycology 46(1):36–44.
Aslan, Sebnem and Ilgi Karapinar Kapdan. 2006. “Batch Kinetics of Nitrogen and Phosphorus Removal from Synthetic Wastewater by Algae Batch Kinetics of Nitrogen and Phosphorus Removal from Synthetic Wastewater by Algae.” (November).
Bashir, Khawaja Muhammad Imran, Moo Sang Kim, Ulf Stahl, and Man Gi Cho. 2018. “Agrobacterium-Mediated Genetic Transformation of Dictyosphaerium Pulchellum for the Expression of Erythropoietin.” Journal of Applied Phycology 30(6):3503–18.
Béchet, Quentin, Martin Laviale, Nicolas Arsapin, Hubert Bonnefond, and Olivier Bernard. 2017. “Modeling the Impact of High Temperatures on Microalgal Viability and Photosynthetic Activity.” Biotechnology for Biofuels 10(1):1–11.
Bourras, Salim, Thierry Rouxel, and Michel Meyer. 2015. “Agrobacterium Tumefaciens Gene Transfer : How a Plant Pathogen Hacks the Nuclei of Plant and Nonplant Organisms.”
Bradley, I. M. and J. S. Guest. 2017. “Influence of Solids Residence Time and Carbon Storage on Nitrogen and Phosphorus Recovery by Microalgae across Diel Cycles.” Water Research.
Carvalho, Ana P., Susana O. Silva, José M. Baptista, and F. Xavier Malcata. 2011. “Light Requirements in Microalgal Photobioreactors: An Overview of Biophotonic Aspects.” Applied Microbiology and Biotechnology 89(5):1275–88.
Cheirsilp, Benjamas and Salwa Torpee. 2012. “Enhanced Growth and Lipid Production of Microalgae under Mixotrophic Culture Condition: Effect of Light Intensity, Glucose Concentration and Fed-Batch Cultivation.” Bioresource Technology 110:510–16.
Chisti, Yusuf. 2007. “Biodiesel from Microalgae.” Biotechnology Advances 25(3):294–306.
Coll, J. M. 2006. “Review. Methodologies for Transferring DNA into Eukaryotic Microalgae.” Spanish Journal of Agricultural Research 4(4):316–30.
Converti, Attilio, Alessandro A. Casazza, Erika Y. Ortiz, Patrizia Perego, and Marco Del Borghi. 2009. “Effect of Temperature and Nitrogen Concentration on the Growth and Lipid Content of Nannochloropsis Oculata and Chlorella Vulgaris for Biodiesel Production.” Chemical Engineering and Processing: Process Intensification 48(6):1146–51.
Daliry, S., A. Hallajisani, J. Mohammadi Roshandeh, H. Nouri, and A. Golzary. 2017. “Investigation of Optimal Condition for Chlorella Vulgaris Microalgae Growth.” Global Journal of Environmental Science and Management 3(2):217–30.
Demirbas, M. Fatih. 2011. “Biofuels from Algae for Sustainable Development.” Applied Energy 88(10):3473–80.
Gong, Qitao, Yuanzheng Feng, Ligai Kang, Mengyuan Luo, and Junhong Yang. 2014. “Effects of Light and PH on Cell Density of Chlorella Vulgaris.” Energy Procedia 61:2012–15.
Hallmann, Armin. 2007. “Algal Transgenics and Biotechnology.” Transgenic Plant J 1(1):81–98.
Haselkorn, Robert. 1999. “The Complete Chloroplast DNA Sequence of the Green Alga Nephroselmis Olivacea : Insights into the Architecture of Ancestral Chloroplast Genomes.” 96(August):10248–53.
Hwang, Authors, Hau-hsuan Hwang, Manda Yu, and Erh-min Lai. 2020. “Agrobacterium-Mediated Plant Transformation : Biology and Applications Published By : The American Society of Plant Biologists Agrobacterium- Mediated Plant Transformation : Biology and Applications.” 2017(15).
Jacob-Lopes, Eduardo, Carlos Henrique Gimenes Scoparo, Lucy Mara Cacia Ferreira Lacerda, and Telma Teixeira Franco. 2009. “Effect of Light Cycles (Night/Day) on CO2 Fixation and Biomass Production by Microalgae in Photobioreactors.” Chemical Engineering and Processing: Process Intensification 48(1):306–10.
Juneja, Ankita, Ruben Michael Ceballos, and Ganti S. Murthy. 2013. “Effects of Environmental Factors and Nutrient Availability on the Biochemical Composition of Algae for Biofuels Production: A Review.” Energies 6(9):4607–38.
Kathiresan, S., A. Chandrashekar, G. A. Ravishankar, and R. Sarada. 2009. “Agrobacterium-Mediated Transformation in the Green Alga Haematococcus Pluvialis (Chlorophyceae, Volvocales).” Journal of Phycology 45(3):642–49.
Khalil, Zeinab I., Mohsen M. S. Asker, Salwa El-Sayed, and Imam A. Kobbia. 2010. “Effect of PH on Growth and Biochemical Responses of Dunaliella Bardawil and Chlorella Ellipsoidea.” World Journal of Microbiology and Biotechnology 26(7):1225–31.
Khan, Muhammad Imran, Jin Hyuk Shin, and Jong Deog Kim. 2018. “The Promising Future of Microalgae: Current Status, Challenges, and Optimization of a Sustainable and Renewable Industry for Biofuels, Feed, and Other Products.” Microbial Cell Factories 17(1):1–21.
Khoeyi, Zahra Amini, Jafar Seyfabadi, and Zohreh Ramezanpour. 2012. “Effect of Light Intensity and Photoperiod on Biomass and Fatty Acid Composition of the Microalgae, Chlorella Vulgaris.” Aquaculture International 20(1):41–49.
Kitaya, Y., H. Azuma, and M. Kiyota. 2005. “Effects of Temperature, CO 2 /O 2 Concentrations and Light Intensity on Cellular Multiplication of Microalgae, Euglena Gracilis.” Advances in Space Research 35(9 SPEC. ISS.):1584–88.
Klebe, Robert J., June V Harriss, Z. Dave Sharp, and Michael G. Douglas. 1983. “A General Method for Polyethylene-Glycol-Induced Genetic Transformation of Bacteria and Yeast Many Genetically Important Species Are Not Capable , under Natural Conditions , of Undergoing Genetic Transformation ( Venema , 1979 ). However , Protoplasts Of .” 25:333–41.
Kong, Weibao, Hao Song, Yuntao Cao, Hong Yang, Shaofeng Hua, and Chungu Xia. 2011. “The Characteristics of Biomass Production , Lipid Accumulation and Chlorophyll Biosynthesis of Chlorella Vulgaris under Mixotrophic Cultivation.” 10(55):11620–30.
Kumar, Sasidharanpillai Vinod, Rachel William Misquitta, Vanga Siva Reddy, Basuthkar Jagadeeswar Rao, and Manchikatla Venkat Rajam. 2004. “Genetic Transformation of the Green Alga - Chlamydomonas Reinhardtii by Agrobacterium Tumefaciens.” Plant Science 166(3):731–38.
Lam, Man Kee and Keat Teong Lee. 2012. “Potential of Using Organic Fertilizer to Cultivate Chlorella Vulgaris for Biodiesel Production.” Applied Energy 94:303–8.
Leliaert, Frederik, David R. Smith, Hervé Moreau, Matthew D. Herron, Heroen Verbruggen, Charles F. Delwiche, and Olivier De Clerck. 2012. “Phylogeny and Molecular Evolution of the Green Algae.” Critical Reviews in Plant Sciences 31(1):1–46.
Leliaert, Frederik, David R Smith, Hervé Moreau, Matthew D. Herron, Heroen Verbruggen, Charles F. Delwiche, Olivier De Clerck, Frederik Leliaert, David R. Smith, Hervé Moreau, Matthew D. Herron, Heroen Verbruggen, Charles F. Delwiche, Olivier De Clerck Phylogeny, Frederik Leliaert, David R. Smith, Matthew D. Herron, Heroen Verbruggen, Charles F. Delwiche, and Olivier De Clerck. 2012. “Critical Reviews in Plant Sciences Phylogeny and Molecular Evolution of the Green Algae Phylogeny and Molecular Evolution of the Green Algae.” 2689.
Leliaert, Frederik, Ana Tronholm, Claude Lemieux, Monique Turmel, Michael S. Depriest, Debashish Bhattacharya, Kenneth G. Karol, Suzanne Fredericq, Frederick W. Zechman, and Juan M. Lopez-bautista. 2016. “Chloroplast Phylogenomic Analyses Reveal the Deepest-Branching Lineage of the Chlorophyta , Palmophyllophyceae Class . Nov .” Nature Publishing Group (February):1–13.
Leliaert, Frederik, Heroen Verbruggen, and Frederick W. Zechman. 2011. “Into the Deep: New Discoveries at the Base of the Green Plant Phylogeny.” BioEssays 33(9):683–92.
Lemieux, Claude, Christian Otis, and Monique Turmel. 2007. “A Clade Uniting the Green Algae Mesostigma Viride and Chlorokybus Atmophyticus Represents the Deepest Branch of the Streptophyta in Chloroplast Genome-Based Phylogenies.” 17.
Lewis, Louise A. and Richard M. McCourt. 2004. “Green Algae and the Origin of Land Plants.” American Journal of Botany 91(10):1535–56.
Liu, Shuangwei, Jingjing Ma, Hongmei Liu, Yingtian Guo, Wei Li, and Shihui Niu. 2020. “An Efficient System for Agrobacterium-Mediated Transient Transformation in Pinus Tabuliformis.” Plant Methods 16(1):1–9.
Liu, Zhi-yuan and Guang-ce Wang. 2008. “Effect of Iron on Growth and Lipid Accumulation in Chlorella Vulgaris.” 99:4717–22.
Lum, Krystal K., Jonggun Kim, and Xin G. Lei. 2013. “Dual Potential of Microalgae as a Sustainable Biofuel Feedstock and Animal Feed.” Journal of Animal Science and Biotechnology 4(1):1–7.
Luo, W., L. Krienitz, S. Pflugmacher, and N. Walz. 2005. “ Genus and Species Concept in Chlorella and Micractinium (Chlorophyta, Chlorellaceae): Genotype versus Phenotypical Variability under Ecosystem Conditions .” SIL Proceedings, 1922-2010 29(1):170–73.
Luo, Wei, Stephan Pflugmacher, Thomas Pröschold, Norbert Walz, and Lothar Krienitz. 2006. “Genotype versus Phenotype Variability in Chlorella and Micractinium (Chlorophyta, Trebouxiophyceae).” Protist 157(3):315–33.
Lv, Jian-ming, Li-hua Cheng, Xin-hua Xu, Lin Zhang, and Huan-lin Chen. 2010. “Bioresource Technology Enhanced Lipid Production of Chlorella Vulgaris by Adjustment of Cultivation Conditions.” Bioresource Technology 101(17):6797–6804.
Maliga, Pal, ed. 2014. Chloroplast Biotechnology Methods and Protocols. Vol. 1132. HUMAN PRESS.
Martins, A., Nidia S. Caetano, and Teresa M. Mata. 2010. “Microalgae for Biodiesel Production and Other Applications : A Review.” 14:217–32.
McCullen, Colleen A. and Andrew N. Binns. 2006. “Agrobacterium Tumefaciens and Plant Cell Interactions and Activities Required for Interkingdom Macromolecular Transfer.” Annual Review of Cell and Developmental Biology 22:101–27.
Mini, Paola, Olivia Costantina Demurtas, Silvia Valentini, Patrizia Pallara, Giuseppe Aprea, Paola Ferrante, and Giovanni Giuliano. 2018. “Agrobacterium-Mediated and Electroporation-Mediated Transformation of Chlamydomonas Reinhardtii: A Comparative Study.” BMC Biotechnology 18(1):1–12.
Moczydlowska, Malgorzata, Ed Landing, Wenlong Zang, and Teodoro Palacios. 2011. “Proterozoic Phytoplankton and Timing of Chlorophyte Algae Origins.” Palaeontology 54(4):721–33.
Muñoz, Camilo F., Mark H. J. Sturme, Sarah D’Adamo, Ruud A. Weusthuis, and René H. Wijffels. 2019. “Stable Transformation of the Green Algae Acutodesmus Obliquus and Neochloris Oleoabundans Based on E. Coli Conjugation.” Algal Research 39(January).
Nester, Eugene W. 2015. “Agrobacterium: Nature’s Genetic Engineer.” Frontiers in Plant Science 5(JAN):1–16.
Ng, I. Son, Shih I. Tan, Pei Hsun Kao, Yu Kaung Chang, and Jo Shu Chang. 2017. “Recent Developments on Genetic Engineering of Microalgae for Biofuels and Bio-Based Chemicals.” Biotechnology Journal 12(10):1–13.
Pawlik-skowron, Barbara and Magdalena Trzcin. 2014. “Influence of Photoperiods on the Growth Rate and Biomass Productivity of Green Microalgae.” 735–41.
Pitsch, Gianna, Lubomír Adamec, Sebastian Dirren, Frank Nitsche, Karel Šimek, Dagmara Sirová, and Thomas Posch. 2017. “The Green Tetrahymena Utriculariae n. Sp. (Ciliophora, Oligohymenophorea) with Its Endosymbiotic Algae (Micractinium Sp.), Living in Traps of a Carnivorous Aquatic Plant.” Journal of Eukaryotic Microbiology 64(3):322–35.
Potvin, Gabriel and Zisheng Zhang. 2010. “Strategies for High-Level Recombinant Protein Expression in Transgenic Microalgae: A Review.” Biotechnology Advances 28(6):910–18.
Pratheesh, P. T., G. M. Shonima, J. Thomas, C. I. Abraham, and G. Muraleedhara Kurup. 2012. “Study on Efficacy of Different Agrobacterium Tumefaciens Strains in Genetic Transformation of Microalga Chlamydomonas Reinhardtii.” Advances in Applied Science Research 3(5):2679–86.
Pratheesh, P. T., M. Vineetha, and G. Muraleedhara Kurup. 2014. “An Efficient Protocol for the Agrobacterium-Mediated Genetic Transformation of Microalga Chlamydomonas Reinhardtii.” Molecular Biotechnology 56(6):507–15.
Pröschold, Thomas, Gianna Pitsch, and Tatyana Darienko. 2020. “Micractinium Tetrahymenae (Trebouxiophyceae, Chlorophyta), a New Endosymbiont Isolated from Ciliates.” Diversity 12(5).
Pulz, Otto and Wolfgang Gross. 2004. “Valuable Products from Biotechnology of Microalgae.” Applied Microbiology and Biotechnology 65(6):635–48.
Ramessur, Anusha Devi, John H. Bothwell, Christine A. Maggs, Sook Yee Gan, and Siew Moi Phang. 2018. “Agrobacterium-Mediated Gene Delivery and Transient Expression in the Red Macroalga Chondrus Crispus.” Botanica Marina 61(5):499–510.
Randrianarison, Gilbert and Muhammad Aqeel Ashraf. 2017. “Microalgae: A Potential Plant for Energy Production.” Geology, Ecology, and Landscapes 1(2):104–20.
Rathod, Jayant Pralhad, Gunjan Prakash, Reena Pandit, and Arvind M. Lali. 2013. “Agrobacterium-Mediated Transformation of Promising Oil-Bearing Marine Algae Parachlorella Kessleri.” Photosynthesis Research 118(1–2):141–46.
Rene H.Wijffels, J. Mari. Barbosa. 2010. “Perspective. An Outlook on Microalgal Biofuels.” Science
Vol. 329(August):796–99.
Richmond, Amos. 2004. “Handbook of Microalgal Culture.”
Robert A Andersen. 2005. Algal Culturing Techniques.
Salvucci, Michael E. and Steven J. Crafts-Brandner. 2004. “Relationship between the Heat Tolerance of Photosynthesis and the Thermal Stability of Rubisco Activase in Plants from Contrasting Thermal Environments.” Plant Physiology 134(4):1460–70.
San, Thye, Cha Willy, and Yee Ahmad. 2012. “Assessment of Factors Affecting Agrobacterium -Mediated Genetic Transformation of the Unicellular Green Alga , Chlorella Vulgaris.” 1771–79.
Sanitha, Mary, Sudhakar Radha, Anwar Aliya Fatima, Selvaraju Gayathri Devi, and Mohandass Ramya. 2014. “Agrobacterium-Mediated Transformation of Three Freshwater Microalgal Strains.” Polish Journal of Microbiology 63(4):387–92.
Sekimoto, Hiroyuki. 2017. “Sexual Reproduction and Sex Determination in Green Algae.” Journal of Plant Research 130(3):423–31.
Sforza, Eleonora, Diana Simionato, Giorgio Mario Giacometti, Alberto Bertucco, and Tomas Morosinotto. 2012. “Adjusted Light and Dark Cycles Can Optimize Photosynthetic Efficiency in Algae Growing in Photobioreactors.” 7(6).
Shah, Md Mahfuzur R., Yuanmei Liang, Jay J. Cheng, and Maurycy Daroch. 2016. “Astaxanthin-Producing Green Microalga Haematococcus Pluvialis: From Single Cell to High Value Commercial Products.” Frontiers in Plant Science 7(APR2016).
Shilton, Andy, Oliver B. Fringer, and Raul Mun. 2010. “Mechanistic Modeling of Broth Temperature in Outdoor Photobioreactors.” 44(6):2197–2203.
Siemering, Kirby R., Ralph Golbik, Richard Sever, and Jim Haseloff. 1996. “Mutations That Suppress the Thermosensitivity of Green Fluorescent Protein.” Current Biology 6(12):1653–63.
Singh, Anoop and Stig Irving Olsen. 2013. “A Critical Review of Biochemical Conversion, Sustainability and Life-Cycle Assessment of Algal Biofuels [Applied Energy 88 (2011) 3548-55].” Applied Energy 101:822.
Singh, S. P. and Priyanka Singh. 2015. “Effect of Temperature and Light on the Growth of Algae Species: A Review.” Renewable and Sustainable Energy Reviews 50:431–44.
Sun, Linhua, Ling Fang, Zhenhua Zhang, Xin Chang, David Penny, and Bojian Zhong. 2016. “Chloroplast Phylogenomic Inference of Green Algae Relationships.” Scientific Reports 6(October 2015):1–6.
Suttangkakul, Anongpat, Anchalee Sirikhachornkit, Piyada Juntawong, Wilasinee Puangtame, Thitikorn Chomtong, Suchada Srifa, Sukhita Sathitnaitham, Wasawat Dumrongthawatchai, Kanidtha Jariyachawalid, and Supachai Vuttipongchaikij. 2019. “Evaluation of Strategies for Improving the Transgene Expression in an Oleaginous Microalga Scenedesmus Acutus.” BMC Biotechnology 19(1):1–15.
Tan, Jia Sen, Sze Ying Lee, Kit Wayne Chew, Man Kee Lam, Jun Wei Lim, Shih Hsin Ho, and Pau Loke Show. 2020. “A Review on Microalgae Cultivation and Harvesting, and Their Biomass Extraction Processing Using Ionic Liquids.” Bioengineered 11(1):116–29.
Wang, Meng and Chul Park. 2015. “Investigation of Anaerobic Digestion of Chlorella Sp. and Micractinium Sp. Grown in High-Nitrogen Wastewater and Their Co-Digestion with Waste Activated Sludge.” Biomass and Bioenergy 80(813):30–37.
Worden, Alexandra Z., Jae-hyeok Lee, Thomas Mock, Pierre Rouzé, Melinda P. Simmons, Andrea L. Aerts, Andrew E. Allen, Marie L. Cuvelier, Evelyne Derelle, Meredith V Everett, Elodie Foulon, Jane Grimwood, Heidrun Gundlach, Bernard Henrissat, Carolyn Napoli, Jonathan H. Badger, Pedro M. Coutinho, Elif Demir, Inna Dubchak, Chelle Gentemann, Wenche Eikrem, Jill E. Gready, Uwe John, William Lanier, Erika A. Lindquist, Olivier Panaud, Jasmyn Pangilinan, Ian Paulsen, Benoit Piegu, and Aaron Poliakov. 2009. “The Marine Picoeukaryotes Micromonas.” 9375(April).
Wu, Huanyang. 2016. “Effect of Different Light Qualities on Growth, Pigment Content, Chlorophyll Fluorescence, and Antioxidant Enzyme Activity in the Red Alga Pyropia Haitanensis (Bangiales, Rhodophyta).” BioMed Research International 2016.
Zhang, Ru, Weronika Patena, Ute Armbruster, Spencer S. Gang, Sean R. Blum, and Martin C. Jonikas. 2014. “High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA.” Plant Cell 26(4):1398–1409.
Zuccaro, Gaetano, Abu Yousuf, Antonino Pollio, and Jean Philippe Steyer. 2019. Microalgae Cultivation Systems. Elsevier Inc.
指導教授 呂俊毅 葉淑丹(Jun Yi Leu Shu Dan Yeh) 審核日期 2021-7-30
推文 facebook   plurk   twitter   funp   google   live   udn   HD   myshare   reddit   netvibes   friend   youpush   delicious   baidu   
網路書籤 Google bookmarks   del.icio.us   hemidemi   myshare   

若有論文相關問題,請聯絡國立中央大學圖書館推廣服務組 TEL:(03)422-7151轉57407,或E-mail聯絡  - 隱私權政策聲明