近紫外光結構照明顯微術應用於花瓣表面光學特性之研究

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藍聖荃(Sheng-Chiuan-Lan) 查詢紙本館藏

畢業系所

光電科學與工程學系

論文名稱

近紫外光結構照明顯微術應用於花瓣表面光學特性之研究
(Apply Near-ultraviolet Structured Illumination Microscopy to Studies of Surface Structures and Optical Characteristics of the Petals)

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至系統瀏覽論文 (2026-12-9以後開放)

摘要(中)

視覺上，受日光照射的花瓣如何吸引昆蟲是一個研究人員想解開的難題，因此花瓣表面的光學特性成為研究的目標之一，現有的花瓣顯微系統無法取得花瓣的當下，觀察其表面的微結構，因此樣本需要使用化學藥劑複製表面結構或是保存摘取的花瓣於容器中，本研究設計一套保留花瓣原始樣貌的顯微系統，此系統使用近紫外光作為光源，在不破壞花瓣表面結構下，利用結構照明顯微術拍攝花辮表面的切片資訊，重建的影像可組成一組帶有螢光與散射光的三維結構影像。另外，為了確認近紫外光照射花瓣表面結構的表面散射訊號的分布，因此架設一個拍攝全觀花瓣的 UV 光影像作為微觀影像的對照組。本研究利用上述兩種系統對四種花瓣進行觀測，分別為大白菊、大花咸豐草、黑斑龍膽與向日葵，透過顯微系統可觀察黑斑龍膽與向日葵花瓣由內而外的細胞外觀有形變，另外兩種花瓣則沒有形變，量化四種微觀花瓣細胞的長寬比、週期與光強度，花瓣表面微結構的形變與全觀 UV 光的散射訊號分布不一定有正向關係；將微觀螢光、微觀散射光與全觀 UV 光強度互相比較，上述三種光強度沒有一致的相關性。

摘要(英)

Visually, how petals irradiated by sunlight attract insects is a complex issue that researchers are curious about,accordingly, the optical properties of petals′ surfaces have become one of research topics. The existing petal microscopy system can′t obtain the current petals and observe the surface microstructure, so the sample needs to use chemicals to replicate the surface structure or preserve the extracted petals in a container. This study set up a microscopy which preserves the original appearance of the petals. This system uses near-ultraviolet light as the light source. Under the surface structure of the petals, structured illumination microscopy is used to capture the slice information of the surface of the flower braid. The reconstructed image can form a set of three-dimensional structure images with fluorescence and scattering. In addition, in order to confirm the distribution of surface scattering on the surface structure of petals irradiated by near-ultraviolet light, a UV light image of the petals was set up as a control group for microscopic images. In this study, the above two systems were used to observe four petals, namely, Chrysanthemum × morifolium, Bidens Pilosa var. radiata, Gentiana scabrida var. punctulata and Helianthus annuus. Through the microscope system, the cells of black spotted gentian and sunflower petals can be observed from the inside to the outside. The appearance is deformed, while the other two petals have no deformation. To quantify the aspect ratio, period, and light intensity of the four microscopic petal cells, the deformation of the petal surface microstructure may not have a positive relationship with the distribution of the overall UV light scattering signal; Comparing the intensity of microscopic fluorescence, microscopic scattered light, and full-view UV light with each other, there is no consistent correlation between the above three light intensities.

關鍵字(中)

★ 結構照明顯微術
★ 花瓣的表面結構
★ 花瓣的光學特性

關鍵字(英)

★ structured illumination microscopy
★ surface structures of the petals
★ optical characteristics of the petals

論文目次

摘要 i
Abstract i
致謝 i
目錄 ii
第一章緒論 1
1.1 研究動機與目的 1
1.2 文獻回顧與探討 1
1.2.1 電子式顯微系統 1
1.2.2 光學顯微系統 3
1.2.3花瓣表面光學特性 7
1.3 論文架構 10
第二章實驗原理 11
2.1 花瓣組織成分 11
2.2 庫貝卡蒙克理論 12
2.3 結構照明顯微術 14
2.4 螢光 16
第三章系統架構 18
3.1 nUVSIM系統 18
3.2 系統的空間解析度 20
3.2 花瓣螢光光譜系統 24
3.3 全觀的拍攝系統 25
3.4 花瓣簡介、採集過程與保存方式 26
3.4.1 黑斑龍膽 26
3.4.2 大花咸豐草 26
3.4.3 向日葵 27
3.4.4 大白菊 27
3.5 備製樣本流程 28
3.5.1 微結構樣本 28
3.5.2 全觀拍攝花瓣樣本 29
第四章實驗結果 30
4.1 螢光與散射光的重建影像與螢光光譜 30
4.1.1 影像重建 30
4.1.2 散射光與螢光影像疊加 32
4.1.3 影像尺寸 34
4.1.4 螢光光譜 36
4.2 花瓣細胞長寬比 37
4.3 花瓣細胞頂端的主頻率 39
4.4 微觀與全觀花瓣影像強度對比 42
第五章結論 46
參考文獻 47

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指導教授

陳思妤(Szu-Yu Chen)

審核日期

2022-1-6

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