摘要(英) |
Multiplex PCR is the term used when more than one pair of primers is used in a polymerase chain reaction. The goal of multiplex PCR is to amplify several segments of target DNA simultaneously and thereby to conserve template DNA, save time, and minimize expense. The success of above-mentioned methods is dependent on primer design. However, this is a tedious task as too many constrains such as melting temperatures, primer length, GC content and complementarity to optimize the PCR product needs to be satisfied. Various kinds of approaches for designing a primer have been proposed in the last few decades, but most of them don’t satisfy the multiplex PCR. The system draws on genetic algorithm which imitates nature’s process of evolution and genetic operations on chromosomes in order to achieve the optimal solutions. Presently experimental results indicate that the proposed algorithm can find some group of primer pairs that not only obey the design properties but also work at same tube. |
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