抗菌脂肽與類細菌脂質膜在最低抑制濃度下的相互作用

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姓名

陳冠倫(Guan-Lun Chen) 查詢紙本館藏

畢業系所

化學工程與材料工程學系

論文名稱

抗菌脂肽與類細菌脂質膜在最低抑制濃度下的相互作用
(Interactions between antimicrobial lipopeptides and bacteria-mimetic lipid membranes across the minimum inhibitory concentrations)

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至系統瀏覽論文 (2027-9-30以後開放)

摘要(中)

細菌的抗生素耐藥性是對治療的威脅。科學家們希望找到一種替代療法來解決這個問題。抗菌脂肽被認為是替代傳統抗生素的有潛力療法，因為它們對細菌耐藥性的敏感性較低。在最小抑菌濃度抗菌成本較低，我們的實驗選擇最小抑菌濃度來研究抗菌脂肽彈性性質的變化。更具體地說，透過動態光散射儀、螢光光譜儀和小角度 X 光散射實驗，我們研究不同的抗菌脂肽如何改變彈性和結構特性，包括彎曲係數、自發曲率（即彎曲趨勢）和脂質膜的厚度。實驗結果指出在最小抑菌濃度下抗菌脂肽對於單層囊泡的彈性性質，彎曲係數、自發曲率和膜厚無明顯變化，並且彈性自由能也沒有顯著差異。

摘要(英)

Antibiotic resistance of bacteria is a threat to treatment. Scientists want to find an alternative therapy to solve this issue. Antimicrobial lipopeptides have been considered as a promising alternative to conventional antibiotics, due to their lower vulnerability to bacterial drug resistance. Our experiment chose the MIC (minimum inhibitory concentration) to investigate the change of elastic properties in antimicrobial lipopeptide for lower cost. More specifically, by dynamic light scattering, fluorescent spectroscopy, and small-angle X-ray scattering, we examine how the different antimicrobial lipopeptides alter the elastic and structural properties, including bending stiffness, spontaneous curvature (i.e., the tendency of curving) and membrane thickness of the membranes. The results of the experiment pointed out that the elastic property bending stiffness, spontaneous curvature and membrane thickness not changed significantly in MIC, and there is no significant difference in the elastic free energy.

關鍵字(中)

★ 抗菌脂肽
★ 最小抑菌濃度
★ 彈性自由能

關鍵字(英)

★ antimicrobial lipopeptides
★ minimum inhibitory concentration
★ elastic free energy

論文目次

摘要……………………………………………………………………….I
Abstract……………………………………………………..……………II
致謝……………………………………………………………………..III
目錄……………………………………………………………………..IV
圖目錄………………………………………………………………...VIII
表目錄………………………………………………………………….XII
第一章緒論…………………………………………………………......1
1.1抗菌肽（Antimicrobial peptides）…………………………...…3
1.2細胞膜（Plasma membrane）…………………………………...6
1.3磷脂質（Phospholipids）……………………….………...…….7
1.4彈性性質（Elastic properties）………………………………..10
1.4.1自發曲率（Spontaneous curvature）……………...............12
1.4.2彎曲係數（Bending modulus）………………...................14
1.5研究動機………………………………………………………..16
第二章實驗材料及方法………………………………...…….............17
2.1實驗材料………………………………………………………..17
2.1.1實驗用磷脂質……………………………….......................17
2.1.1.1 磷脂質（Phospholipid）…………………..................17
2.1.1.2螢光磷脂質（Fluorescent phospholipid）………...…19
2.1.2抗菌脂肽（Antimicrobial lipopeptide）……………………20
2.1.3緩衝溶液（Buffer）………………………………………...28
2.1.4其他材料……………………………………….....................29
2.2實驗設備…………………………………………………………30
2.3實驗步驟及原理…………………………………………………31
2.3.1單層囊泡製備………………………………….....................31
2.3.2抗菌脂肽溶液製備…………………………….....................32
2.3.3動態光散射（Dynamic light scattering）…………………..33
2.3.3.1動態光散射原理………………………………………..33
2.3.3.2測量彎曲係數原理……………...……………………...34
2.3.3.3動態光散射儀實驗步驟…………………………..……36
2.3.4螢光光譜儀（Fluorescence spectroscopy）………………..37
2.3.4.1螢光光譜儀原理………………………………………..37
2.3.4.2測量自發曲率原理……………………………………..37
2.3.4.3外層螢光還原實驗步驟………………………………..40
2.3.5小角度X光散射（Small angle X-ray scattering）……......42
2.3.5.1小角度X光散射原理……………………………...…..42
2.3.5.2小角度X光散射實驗步驟…………………….....……43
2.3.6圓二色光譜（Circular dichroism）………………………...44
2.3.6.1圓二色光譜原理…………………………………..……44
2.3.6.2圓二色光譜實驗步驟…………………………………..45
2.4數據處理…………………………………………………………46
2.4.1量化彎曲係數…………………………………….................46
2.4.2量化自發曲率…………………………………….................49
2.4.3單層囊泡膜厚…………………………………….................51
第三章結果……………………………………………………………54
3.1抗菌脂肽對單層囊泡粒徑大小及分佈的影響…………………54
3.2抗菌脂肽對單層囊泡彎曲係數的影響…………………………57
3.3抗菌脂肽對單層囊泡結構的影響………………………...…….64
3.4抗菌脂肽對單層囊泡膜厚的影響………………………………67
3.5抗菌脂肽對單層囊泡自發曲率的影響…………………………74
3.6抗菌脂肽對單層囊泡二級結構的影響…………………………80
第四章討論…………………………………………………...……….83
4.1彈性性質及膜厚對抗菌脂肽最小抑菌濃度探討………………83
4.2彈性自由能與抗菌脂肽最小抑菌濃度探討……………………85
4.3最小抑菌濃度造成抗菌的其他方向……………………………87
第五章結論………………………………………………...….............89
文獻目錄與參考資料…………………………………………………..90
附錄……………………………………………………………………..96

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指導教授

陳儀帆(Yi-Fan Chen)

審核日期

2022-9-29

推文