博碩士論文 110324068 詳細資訊




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姓名 陳佳吟(Jia-Yin Chen)  查詢紙本館藏   畢業系所 化學工程與材料工程學系
論文名稱 分子自組裝結構對雙離子高分子醫療塗層穩定性與抗汙功能的影響
(Effect of Molecular Self-assembly on Stability and Antifouling Properties of Polyzwitterion Medical Coatings)
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檔案 [Endnote RIS 格式]    [Bibtex 格式]    [相關文章]   [文章引用]   [完整記錄]   [館藏目錄]   至系統瀏覽論文 (2028-7-31以後開放)
摘要(中) 316 不銹鋼是一種廣泛應用的醫療級不銹鋼,適用於手術設備、食品行業、沿海設施、廚具等領域。由於在 316 不銹鋼中添加了鉬,其耐腐蝕性和高溫強度得到了很大的提高。以往的研究已經通過表面修飾來擴大基材的應用範圍,比如將矽片上應用矽烷、金表面上應用磷酸等。具有不同頭基和不同烷基鏈長的分子已被廣泛應用於許多不同的金屬表面。在本研究中,我們合成了三種具有不同烷基鏈的羧酸丙烯醯胺分子,分別是丙烯醯胺基乙酸 (2-AE)、6-丙烯醯胺基己酸 (6-AH) 和 11-丙烯醯胺基十一酸 (11-AU)。在單體的修飾過程中,存在兩個區域:(1) 極性丙烯醯胺 (2) 疏水烷基鏈。這兩個區域之間的相互作用將決定整體結構和有序性。通過氫鍵作用,內部二級醯胺被用來控制分子定向和提高單層穩定性。此外,丙烯酸酯結構可以通過聚合技術構建具有可調性性質的各種分子結構。2-甲基丙烯酰氧乙基磷酸膽鹼 (MPC) 具有抗菌和抗污染的作用。然而,它缺乏機械穩定性。為了提高穩定性,一種好的方法是將我們合成的三種單體進行聚合。核磁共振光譜 (1H NMR) 可用於表徵三種不同單體的化學結構。此外,我們可以通過循環伏安法 (CV) 和傅立葉轉換紅外光譜 (FT-IR) 了解不同烷基鏈的羧酸丙烯醯胺分子在 316 不銹鋼上的結合親和力。在與 MPC 進行聚合並修飾在 316 不銹鋼上之後,我們可以通過使用 FT-IR、1H NMR、X 射線光電子能譜 (XPS) 和小角度 X 射線散射(SAXS) 來檢測功能基團和分子狀態。最後,我們將修飾後的 316 不銹鋼基材進行細菌和蛋白質貼附測試,確定以哪種高分子修飾會得到最佳效果。
摘要(英) The 316 stainless steel is a widely used medical-grade stainless steel. It suits surgical equipment, the food industry, coastal facilities, kitchenware, etc. Due to the addition of molybdenum to 316 stainless steel, its corrosion resistance and high-temperature strength have been greatly improved. Previous studies have been conducted to expand the application range of substrates by surface modification, such as silane applied to silicon wafers, phosphonic acid applied to gold surfaces, etc. Molecules with different head groups and different alkyl chain lengths have been widely used in many different metal surfaces. In this study, we have synthesized three carboxyl acrylamide with different alkyl chains, acryloylglycine (2-AE), 6-acrylamidohexanoic acid (6-AH), and 11-acrylamidoundecanoic acid (11-AU). For the deposition of monomers, there are two regions:(1) the polar amide and (2) the hydrophobic alkyl chain. The interactions of these two regions will dictate the overall assembly structure and order. Internal secondary amides have been incorporated with the goals of controlling the molecular orientation and improving monolayer stability by hydrogen bonding interactions. Besides, an acrylate structure can build various molecular architectures with tunable properties via the polymerization technique. 2- Methacryloyloxyethyl phosphorylcholine (MPC) has the effect of antibacterial and antifouling. However, it lacks mechanical stability. To improve the stability, a good method is to do the polymerization with the three monomers we synthesized. Nuclear magnetic resonance spectroscopy (1H NMR) characterizes the chemical structure of three different monomers. Furthermore, we can know the binding affinity of carboxylic acid on the 316 stainless steel through cyclic voltammetry (CV) and FT-IR (SR80). After polymerization with MPC and coating on the 316 stainless steel, the functional groups and chemical states can be examined using various techniques such as FT-IR, 1H NMR, X-ray photoelectron spectroscopy (XPS), and Small Angle X-ray Scattering (SAXS). Subsequently, the modified 316 stainless steel substrates can be subjected to bacteria and protein adsorption tests to evaluate the effectiveness of different random copolymers and determine the one with the most favorable outcome.
關鍵字(中) ★ 316 不鏽鋼
★ 自組裝單層膜
★ 羧酸官能基
★ 非特異性吸附
★ 雙離子材料
關鍵字(英) ★ 316 stainless steel
★ self-assembled monolayer
★ carboxylic acid functional group
★ nonspecific adsorption
★ zwitterionic materials
論文目次 目錄
中文摘要 i
Abstract ii
致謝 iv
目錄 v
圖目錄 viii
表目錄 x
一、文獻回顧 1
1-1不鏽鋼316之結構與生醫應用 1
1-2自組裝單層膜 2
1-2-1羧酸官能基與金屬氧化表面之鍵結 3
1-2-2烷基鏈與末端官能基對於自組裝單層膜之影響 5
1-3抗非特異性吸附之材料 8
1-3-1非特異性吸附現象 8
1-3-2抗沾黏材料特性 9
1-3-3雙離子材料 9
1-3-4 PC類雙離子材料 10
二、研究目的 12
三、藥品清單與實驗設備 13
3-1 實驗藥品清單 13
3-2 實驗設備清單 14
3-3 材料合成 15
3-3-1丙烯醯胺基乙酸 (Acryloylglycine, 2-AE) 15
3-3-2丙烯醯胺基己酸 (6-acrylamidohexanoic acid, 6-AH) 15
3-3-3丙烯醯胺基十一酸 (11-(acryloylamino)undecanoic acid, 11-AU) 16
3-3-4合成p(MPC-ran-2-AE) 高分子 16
3-3-5合成p(MPC-ran-6-AH) 高分子 17
3-3-6合成p(MPC-ran-11-AU) 高分子 18
3-4 實驗方法 19
3-4-1自組裝單層膜製備 19
3-4-2水接觸角之量測 (Water contact angle) 19
3-4-3薄膜厚度之量測 (Ellipsometry) 20
3-4-4表面元素之量測 (XPS) 20
3-4-5循環伏安法 (CV curve) 20
3-4-6小角度X光散射 (SAXS) 21
3-4-7臨界微胞濃度測量 (Critical micelle concentration, CMC) 21
3-4-8傅立葉轉換紅外光譜 (FTIR) 22
3-4-9水下摩擦力測試 (Friction test) 22
3-4-10細菌貼附測試 (Bacteria attachment) 23
3-4-11蛋白質貼附測試 (Protein adsorption) 23
3-4-12統計分析 24
四、結果與討論 25
4-1 單體化學結構鑑定與分析 25
4-1-1 丙烯醯胺基羧酸單體 (2-AE、6-AH、11-AU) 之液態核磁共振光譜分析 (1H NMR) 25
4-2比較使用2-AE、6-AH、11-AU進行表面修飾之差異 28
4-2-1丙烯醯胺基羧酸單體 (2-AE、6-AH、11-AU) 表面元素分析 28
4-2-2 丙烯醯胺基羧酸單體 (2-AE、6-AH、11-AU) 水接觸角之量測 29
4-2-3 丙烯醯胺基羧酸單體 (2-AE、6-AH、11-AU) 表面修飾之FTIR圖譜 29
4-2-4 丙烯醯胺基羧酸單體 (2-AE、6-AH、11-AU) 之循環伏安圖 (CV curve) 33
4-3 高分子化學結構鑑定與分析 34
4-3-1 p(MPC-ran-2-AE)、p(MPC-ran-6-AH)、p(MPC-ran-11-AU) 高分子之1H NMR圖譜 34
4-3-2 p(MPC-ran-2-AE)、p(MPC-ran-6-AH)、p(MPC-ran-11-AU) 高分子之數據比較 37
4-3-3 p(MPC-ran-2-AE)、p(MPC-ran-6-AH)、p(MPC-ran-11-AU) 高分子之小角度X光散射數據比較 39
4-3-4 p(MPC5-ran-6-AH5) 與p(MPC5-ran-11-AU5) 臨界微胞濃度 42
4-3-5 p(MPC-ran-2-AE)、p(MPC-ran-6-AH)、p(MPC-ran-11-AU) 高分子之水接觸角之量測 43
4-3-6 p(MPC-ran-2-AE)、p(MPC-ran-6-AH)、p(MPC-ran-11-AU) 高分子之薄膜厚度之量測 44
4-3-7 p(MPC-ran-2-AE)、p(MPC-ran-6-AH)、p(MPC-ran-11-AU) 高分子之表面元素分析 44
4-3-8 p(MPC-ran-2-AE)、p(MPC-ran-6-AH)、p(MPC-ran-11-AU) 高分子之水下摩擦力測試 48
4-3-9 p(MPC-ran-2-AE)、p(MPC-ran-6-AH)、p(MPC-ran-11-AU) 高分子之細菌貼附測試 50
4-3-10 p(MPC-ran-2-AE)、p(MPC-ran-6-AH)、p(MPC-ran-11-AU) 高分子之蛋白質貼附測試 52
五、結論 53
六、未來展望 54
七、參考文獻 55
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指導教授 黃俊仁(Chun-Jen Huang) 審核日期 2023-7-26
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