聚胜肽電解質材料合成及其性質研究分析

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姓名

何敏瑄(Min-Hsuan Ho) 查詢紙本館藏

畢業系所

化學工程與材料工程學系

論文名稱

聚胜肽電解質材料合成及其性質研究分析
(Synthesis and Analysis of Polypeptide Based Polyelectrolyte Complex)

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至系統瀏覽論文 (2029-7-31以後開放)

摘要(中)

目前已有多項研究表明，聚陽離子和聚陰離子形成的聚電解質是很有發展性的一種膠囊技術，可用藥物釋放、製藥、食品、紡織等產業。於是本研究針陰陽離子進行配對，研究其混合後之型態組成及物理特性。
在實驗中，我們使用不同聚合度的聚賴胺酸 (PLL50、PLL100) 作為聚陽離子；聚麩胺酸 (PLGA100) 、聚天門冬胺酸 (PASP90、PASP140、PASP185、PASP250) 作為聚陰離子。將等電荷濃度的聚陽離子和聚陰離子在緩衝溶液中，透過靜電引力及氫鍵形成聚電解質複合體。在顯微鏡觀察下，聚賴胺酸和聚麩胺酸形成的複合體具有固體沉澱結構，而聚賴胺酸和聚天門冬胺酸形成的複合體則具有凝聚層結構。由FTIR及CD分析中，這兩種結構皆分別展現β-sheet和random coil的二級結構。在材料分析方面，發現凝聚複合體具有較弱的氫鍵，並表現出較高的親水性和水合能力。高水合特性也使其將來有機會應用於不沾黏塗層之開發。

摘要(英)

Several studies have shown that polyelectrolyte complex can be a promising encapsulation technique and employed in pharmaceutical, drug delivery, food, and textile industries. Here, we focused on the synthesis and analysis the properties of the polyelectrolyte complex.
In the experiment, we used poly-L-lysine (PLL50, PLL100) as polycations and poly-L-glutamic acid (PLGA100), poly-aspartic acid (PASP90, PASP140, PASP185, PASP250) as polyanions with different degrees of polymerization. Polycations and polyanions of equal charge concentration were mixed in a buffered solution to form polyelectrolyte complexes via electrostatic attraction and hydrogen bonding. Under microscopic observation, the complexes formed by poly-L-lysine and poly-L-glutamic acid exhibited solid precipitates, while those formed by poly-L-lysine and poly-aspartic acid displayed PCs structure. In FTIR and CD analysis, two products exhibited secondary structures of β-sheet and random coil, respectively. After deposition on culture plates, thin film analysis showed that the PCs had weaker hydrogen bonds, higher hydrophilicity and hydration. This property also make it a candidate of non-fouling material for anti-fouling treatment.

關鍵字(中)

★ 聚電解質
★ 聚胜肽凝聚複合體
★ 二級結構

關鍵字(英)

★ polyelectrolyte complex
★ polypeptide complex
★ secondary structure

論文目次

中文摘要 i
Abstract ii
致謝 iii
目錄 iv
圖目錄 vii
表目錄 ix
名詞簡稱 x
聚胜肽電解質代稱 x
一、文獻回顧 1
1-1 聚電解質 1
1-1-1 聚電解質多層薄膜 (Polyelectrolyte multilayer film, PEM film) 2
1-1-2 聚電解質複合體 (Polyelectrolyte complex, PEC) 4
1-1-3 複合凝聚 (Complex coacervate) 6
1-1-4 複合凝聚應用 8
1-2 聚琥珀醯亞胺和聚天門冬胺酸材料 9
1-2-1概述 9
1-2-2 聚天門冬胺酸形成聚電解質複合體 10
二、研究目的 11
三、藥品清單與實驗設備 12
3-1 實驗藥品清單 12
3-2 實驗設備清單 13
3-3 材料合成 14
3-3-1 聚琥珀醯亞胺 (Polysuccinimide, PSI) 14
3-3-2 聚天門冬胺酸鈉鹽 (Poly-aspartic acid sodium salt, PASP) 14
3-4實驗方法 15
3-4-1 聚電解質複合體之製備 15
3-4-2 濁度測定 15
3-4-3 液態核磁共振光譜 (1H NMR) 16
3-4-4 凝膠滲透層析 (GPC) 16
3-4-5 圓二色光譜 (CD) 16
3-4-6 傅立葉轉換紅外光譜 (FTIR) 17
3-4-7 界面電位測定 (Zeta potential) 19
3-4-8 表面元素之量測 (XPS) 19
3-4-9 接觸角之量測 (Contact angle) 19
3-4-10 耗散型石英微量天平 (QCM-D) 20
四、結果討論 21
4-1 聚天門冬胺酸鈉鹽合成性質分析鑑定 21
4-1-1 聚琥珀醯亞胺 (PSI) 之1H NMR圖譜 21
4-1-2 聚天門冬胺酸鈉鹽 (PASP) 之1H NMR圖譜 22
4-1-3 聚天門冬胺酸鈉鹽 (PASP) 之分子量測定 23
4-2 聚電解質複合體性質分析鑑定 24
4-2-1 聚電解質均聚物性質鑑定 24
4-2-2 聚電解質複合體相行為 26
4-2-3 聚電解質複合體二級結構分析 29
4-2-4 聚電解質複合體耐鹽穩定性測試 31
4-2-5 凝聚複合體性質分析 32
4-3 聚電解質複合體塗層性質分析 35
4-3-1 聚電解質複合體塗層表面元素分析 35
4-3-2 聚電解質複合體塗層表面官能基及氫鍵分析 37
4-3-3 聚電解質複合體塗層之水接觸角測定 39
4-3-4 聚電解質複合體塗層之耗散型石英微量天平 (QCM-D) 分析 40
五、結論 43
六、未來展望 44
七、參考文獻 45
八、附錄 49

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指導教授

黃俊仁(Chun-Jen Huang)

審核日期

2024-7-22

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