A WHEP domain regulates the dynamic structure and activity of Caenorhabditis elegans glycyl-tRNA synthetase

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题名:	A WHEP domain regulates the dynamic structure and activity of Caenorhabditis elegans glycyl-tRNA synthetase
作者:	王健家;Chang, Chih-Yao;Chien, Chin-I;Chang, Chia-Pei;Lin, Bo-Chun;Wang, Chien-Chia
贡献者:	生醫理工學院生命科學系
关键词:	aminoacyl tRNA synthetase;Animals;Caenorhabditis elegans (C. elegans);Caenorhabditis elegans - enzymology;Caenorhabditis elegans - genetics;Caenorhabditis elegans Proteins - chemistry;Caenorhabditis elegans Proteins - genetics;Caenorhabditis elegans Proteins - metabolism;Glycine-tRNA Ligase - chemistry;Glycine-tRNA Ligase - genetics;Glycine-tRNA Ligase - metabolism;Mitochondrial Proteins - chemistry;Mitochondrial Proteins - genetics;Mitochondrial Proteins - metabolism;Molecular Bases of Disease;neurodegenerative disease;neurological disease;protein domain;Protein Domains;protein synthesis;Saccharomyces cerevisiae - enzymology;Saccharomyces cerevisiae - genetics;Saccharomyces cerevisiae Proteins - chemistry;Saccharomyces cerevisiae Proteins - genetics;Saccharomyces cerevisiae Proteins - metabolism
日期:	2016-08-05
上传时间:	2026-04-23 11:11:30 (UTC+8)
出版者:	American Society for Biochemistry and Molecular Biology Inc.;United States: Elsevier Inc
摘要:	摘要： WHEP domains exist in certain eukaryotic aminoacyl-tRNA synthetases and play roles in tRNA or protein binding. We present evidence herein that cytoplasmic and mitochondrial forms of Caenorhabditis elegans glycyl-tRNA synthetase (CeGlyRS) are encoded by the same gene (CeGRS1) through alternative initiation of translation. The cytoplasmic form possessed an N-terminal WHEP domain, whereas its mitochondrial isoform possessed an extra N-terminal sequence consisting of an mitochondrial targeting signal and an appended domain. Cross-species complementation assays showed that CeGRS1 effectively rescued the cytoplasmic and mitochondrial defects of a yeast GRS1 knock-out strain. Although both forms of CeGlyRS efficiently charged the cytoplasmic tRNAsGly of C. elegans, the mitochondrial form was much more efficient than its cytoplasmic counterpart in charging the mitochondrial tRNAGly isoacceptor, which carries a defective TψC hairpin. Despite the WHEP domain per se lacking tRNA binding activity, deletion of this domain reduced the catalytic efficiency of the enzyme. Most interestingly, the deletion mutant possessed a higher thermal stability and a somewhat lower structural flexibility. Our study suggests a role for the WHEP domain as a regulator of the dynamic structure and activity of the enzyme. 其他題名： J Biol Chem 出版者： United States: Elsevier Inc 出版日期： 2016-08-05 出處： The Journal of biological chemistry, 2016-08, Vol.291 (32), p.16567-16575 資源來源： Alma/SFX Local Collection 版權： 2016 © 2016 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. 版權： 2016 by The American Society for Biochemistry and Molecular Biology, Inc. 版權： 2016 by The American Society for Biochemistry and Molecular Biology, Inc. 2016 The American Society for Biochemistry and Molecular Biology, Inc. 識別號： ISSN: 0021-9258 識別號： ISSN: 1083-351X 識別號： ISSN: 1067-8816 識別號： EISSN: 1083-351X 識別號： DOI: 10.1074/jbc.M116.730812 識別號： PMID: 27298321
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