本研究以偏振干涉術結合表面電漿共振技術，配合CCD擷取影像，得到四個相位兩兩相差90度的光強度訊號，再以類似四步移相的數值方法，量測出一整面硫醇(Thiol)分佈的全場相位資訊。本解相系統的相位解析度為0.8度，整體系統對折射率的靈敏度為1.6X10-5RIU(Refractive Index Unit, RIU)。此表面電漿共振儀在實驗上可以量測出折射率差0.0001RIU的磷酸鹽緩衝液(Phosphate-Buffered Saline, PBS)。另外可以分辦出濃度為20mM及2mM的硫醇在3x3mm2範圍裡的相位變化。本系統具有即時、定量、高靈敏度等優點，且可應用於微小生物分子鍵結反應的檢測領域上。若能結合不同的生物分子應用於陣列晶片上，將能達到大量平行篩檢的目的。 A full-field phase detection system for surface plasmon resonance (SPR) bio-sensor is presented. In our full-field system, we combine a method of the polarization interference for phase detection, SPR technique and CCD Video camera. By means of a similar algorithm phase-shifting interferometry, the distribution of SPR bio-sensor was obtained. We have successfully detected the refractive index variation of PBS (phosphate-buffered saline) with 0.0001RIU (Refractive Index Unit), and measured the thiol(C8H14O2S2) of 20mM and 2mM within 3×3 mm2 full field range. The resolution of phase detection is about 0.8 degree, and the measurement sensitivity of refractive index is about 1.6×10-5 RIU. This system has advantages, such as immediately, ration, high sensitivity, etc., it can be applied to the small biological molecule interaction. If can combine different biological members to apply to the array chip, it can be a large number of high throughput screening.
