表面電漿共振(Surface Plasmon Resonance，SPR)生物感測器，可在無須對生物分子做任何的標記，由於生物分子間介電常數的微小改變，即時地分析生物分子間的交互作用。由於其技術具有快速、靈敏度高以及大量平行篩檢等優點，可預期將被廣泛地應用在很多生物分子診斷或是物質檢測的領域上。 以往產生表面電漿共振的方式，大部分直接由旋轉機構來達成，然而要產生微小角度的精確變動的旋轉機構所費不貲，因此想出了直線的機構來達成這個目的。利用兩個線性步進馬達，及圓柱面鏡經由反射的原理可製造出微小角度的變化，再利用Mach-Zender相位移干涉的方式，配合PZT、CCD、五步還原法、影像分析重建相位，取代傳統利用反射光強度作為表面電漿共振檢測的方法，再利用P-wave與S-wave檢測金膜的相位比較來驗證此架構的可行性，達到簡單便宜又不失正確性的目的，以期對於表面電漿共振方面的量測結果有更近一步的發展，希望該系統之後能實際應用於生物檢測或氣體分析的機制。 Biomolecular interaction analysis using surface plasmon resonance (SPR) bio-sensor is different from that with traditional fluorescent labeling method. SPR bio-sensor can measure the tiny change of the dielectric constant of analyte promptly. Because this technique has many merits like promptness, high sensitivity, and high-throughput screening , it will be expected to be widely applied in many biomoleular diagnoses or material measurement. The major way to produce SPR in the past was the mechanism of rotating directly , but the fee of this way to produce small accurate rotation of change of angle is not acceptable by us. So we has thought over the system that has straight line structure to reach this purpose. It has two linear stepping motors and the round cylinder mirror to produce the change of the small angle via the reflection principle and then uses the Mach-Zender interferometry to produce interference , cooperating with PZT , CCD , Five-step Algorithm , images analysis to rebuild the phase information , replacing traditional method to use reflective luminous intensity to measure SPR. Moreover , we use P-wave and S-wave laser to test the phase of the gold membrane to prove the feasibility of this system , to achieve the simple , cheap and accurate purpose .We expect that the experimental result has a better development to SPR , and hope the system to be actually applied to the measurement in the biomolecule or the gas.
