所謂的bio-inert表面上細胞的分化與增殖與否及其相關機制尚未被廣泛研究。這些Bio-inert材料普遍應用於胚胎幹細胞(ES cells)及其他幹細胞的培養與保存，其中包含了再生醫學應用的血液幹細胞(hematopioetic stem cells)。我們所發展的生物相容材料是由不同種類柔韌且親水性的奈米Pluronic (PEO-PPO-PEO)片段組成。當臍帶血和胚胎幹細胞被儲存及培養在由奈米Pluronic片段(奈米刷)組成的生物相容表面，培養瓶中的奈米Pluronic片段能誘使幹細胞保存。我們致力於研究組織培養瓶中奈米片段的分子設計及親水性對於血液幹細胞及其他不同種類幹細胞生存能力的影響，以發展再生醫學上幹細胞保存及培養的生物相容材料。我們也計畫培養血液幹細胞以及胚胎幹細胞於含有奈米pluronic片段之組織培養瓶。最後由幹細胞的基因表現來研究含奈米pluronic片段之生物相容材料其中的生物相容機制。我們也計畫發展臍帶血儲存袋(儲存血液幹細胞)，期望以此種表面改質上奈米pluronic片段之儲存袋來增加儲存之血液幹細胞的存活率，並應用於醫療上血液幹細胞之移植以及急性白血病之醫療。本計畫的主要目標有:(1)發展再生醫學上對於多種幹細胞保存及培養最合適的生物相容表面；(2)由幹細胞的基因表現來研究生物相容材料的生物相容機制；(3)藉由生物相容材料的發展以顯示再生醫學中材料工程的重要性。 The surfaces of bio-inert materials on which cells do not proliferate, differentiate nor de-differentiate have not yet been studied extensively. These materials are useful culturing and/or preserving embryonic stem (ES) cells and various other kinds of stem cells, including hematopoietic stem cells for regenerative medicine. The final goal of this project is to develop and investigate the bio-inert materials for the preservation and cultivation of stem cells for regenerative medicine. We develope tissue culture flasks having different types of flexible and hydrophilic nano-segments from several types of Pluronic (PEO-PPO-PEO). We propose that the existence of hydrophilic nano-segments (nano-brushes) of Pluronic on the tissue culture flasks induces bio-inert storage of stem cells, when the umbilical cord blood and ES cells are stored and cultured in the tissue culture flasks having nano-brushes (i.e., Pluronic-immobilized flasks). We investigate the effect of the hydrophilicity and the molecular design of nano-segments of the tissue culture flasks on the viability of various types of stem cells including hematopoietic stem cells to obtain the bio-inert materials for the preservation and cultivation of the stem cells in the regenerative medicine. We further plan to investigate the effect of concentration and length of Pluronic (nano-brushes) on the tissue culture flasks on the viability of hematopoietic stem cells. We also plan to culture not only hematopoietic stem cells but also ES cells on the Pluronic-immobilized tissue culture flasks. Finally, we are planning to investigate the mechanism of the bio-inert characteristics of biomaterials having nano-brush for stem cell survival from gene expression in the stem cells. We are also planning to develop the storage bags for umbilical cord blood (i.e., storage of hematopoietic stem cells) and mesenchymal stem cells for the clinical applications. We are expecting that the increase in viability of hematopoietic stem cells stored in our nano-brush immobilized storage bags will lead to clinical improvements in hematopoietic transfusion and leukemia disease. The goal of this project is (1) to develop the most appropriate bio-inert materials for the preservation and cultivation of various stem cells for regenerative medicine, and (2) to investigate the mechanism of the bio-inert characteristics of biomaterials having nano-brushes for stem cell survival from cell culture and gene expression in the stem cells, and (3) to show the importance of material engineering in the regenerative medicine from the development of bio-inert materials. 研究期間：9908 ~ 10007
