Permeation of blood cells from umbilical cord blood through surface-modified polyurethane foaming membranes

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Title:	Permeation of blood cells from umbilical cord blood through surface-modified polyurethane foaming membranes
Authors:	Higuchi,A;Yang,ST;Li,PT;Ruaan,RC;Chen,WY;Chang,Y;Chang,Y;Tsai,EM;Chen,YH;Wang,HC;Hsu,ST;Ling,QD
Contributors:	化學工程與材料工程學系
Keywords:	HEMATOPOIETIC STEM-CELLS;HUMAN PERIPHERAL-BLOOD;MARROW STROMAL CELLS;EX-VIVO EXPANSION;PROGENITOR CELLS;SEPARATION;ADHESION;MARKERS
Date:	2009
Issue Date:	2010-06-29 17:27:28 (UTC+8)
Publisher:	中央大學
Abstract:	Blood cell permeation of umbilical cord blood (UCB) through surface-modified foamed polyurethane membranes having carboxylic group (PU-COOH) with different pore sizes (i.e., 5, 8.9 and 12 mu m) was investigated. Red blood cells and platelets can permeate through PU-CCCH membranes with any pore sizes analyzed. while leukocytes and hematopoietic stem and progenitor cells (HSPCs) cannot permeate through PU-COOH membranes with permeation ratio of less than 5%. Several recovery solutions (e.g., 20% dextran, 0.5 wt% serum albumin solution, plasma solution) were evaluated for the isolation of several blood cells including HSPCs from PU-COCH membranes after UCB permeated through the membranes. Recovery ratios of leukocytes were relatively low through PU-COOH membranes having pore size (r) = 5 mu m, while higher recovery ratio of RBCs was observed than that of leukocytes. The recovery ratio of HSPCs was found to be 8-27% depending on the membrane pore sizes and recovery solution. The recovery ratio of HSPCs from UCB (8-27% in this study) was found to be less than that from peripheral blood reported previously (60-80% [A. Higuchi, M. Sekiya, Y. Gomei, M. Sakurai, W-Y. Chen, S. Egashira, Y. Matsuoka, Separation of hernatopoietic stem cells from human peripheral blood through modified polyurethane foaming membranes, J. Biomed. Mater. Res.: A 85A (4) (2008) 853-861]). This suggests that the purification of HSPCs form UCB is significantly difficult compared to that from peripheral blood, because of high expression of adhesion proteins of HSPCs in UCB. (C) 2009 Elsevier B.V. All rights reserved.
Relation:	JOURNAL OF MEMBRANE SCIENCE
Appears in Collections:	[National Central University Department of Chemical & Materials Engineering] journal & Dissertation

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