Protein separation by hydrophobic interaction chromatography using methacrylic block copolymers as displacers

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题名:	Protein separation by hydrophobic interaction chromatography using methacrylic block copolymers as displacers
作者:	Ruaan,RC;Hsu,D;Chen,WY;Chen,H;Lin,MS
贡献者:	化學工程與材料工程學系
关键词:	ANION-EXCHANGE;AFFINITY;SALT
日期:	1998
上传时间:	2010-06-29 17:30:02 (UTC+8)
出版者:	中央大學
摘要:	A series of methacrylic triblock copolymers were synthesized. The potential of using these copolymers as displacers or spacers in hydrophobic interaction chromatography (HIC) was discussed. It was found that selected copolymers were able to separate pancreatic trypsin and alpha-chymotrypsin in an Octyl-Sepharose HIC column. The hydrolytic activities of these two enzymes were not affected by the presence of copolymers. The advantages of methacrylic triblock copolymers were their relatively low molecular mass and the reversible precipitability. These properties make the separation between copolymers and proteins easier. The hydrophobicity of the triblock copolymers could be roughly adjusted by controlling the length of each block. But accurate prediction of their hydrophobicity according to the corresponding chemical structure requires further studies. (C) 1998 Elsevier Science BV. All rights reserved.
關聯:	JOURNAL OF CHROMATOGRAPHY A
显示于类别:	[化學工程與材料工程研究所] 期刊論文

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