Microcalorimetric studies of the interactions of imidazole with immobilized Cu(II): Effects of pH value and salt concentration

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题名:	Microcalorimetric studies of the interactions of imidazole with immobilized Cu(II): Effects of pH value and salt concentration
作者:	Wu,CF;Chen,WY;Lee,JF
贡献者:	化學工程與材料工程學系
关键词:	METAL AFFINITY-CHROMATOGRAPHY;SINGLE-STEP PURIFICATION;CHELATE CHROMATOGRAPHY;NICKEL IMINODIACETATE;ESCHERICHIA-COLI;PROTEINS;BINDING;HISTIDINE;ADSORPTION;IMAC
日期:	1996
上传时间:	2010-06-29 17:32:17 (UTC+8)
出版者:	中央大學
摘要:	In this investigation, we measured the influence of pH value and salt concentration on the heat of interaction between imidazole and CS-IDA-Cu(II) gel with a highly sensitive microcalorimeter. The direct enthalpy measurement of the interaction provides thermodynamic information regarding the binding behavior of imidazole toward the immobilized metal ion. The changes in binding enthalpy with the adsorbed amount of imidazole measured at various pH values and salt concentrations are discussed and the binding isotherm is reported. The binding and thermodynamic data obtained in this study can provide information on the mechanism and process of imidazole and protein binding with immobilized metal ions. (C) 1996 Academic Press, Inc.
關聯:	JOURNAL OF COLLOID AND INTERFACE SCIENCE
显示于类别:	[化學工程與材料工程研究所] 期刊論文

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