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    题名: Insecticidal action of mammalian galectin-1 against diamondback moth (Plutella xylostella)
    作者: Chen,SJ;Chen,NT;Wang,SH;Hsu,JC;Ding,WH;Kuo-Huang,LL;Huang,RN
    贡献者: 生命科學研究所
    关键词: GALACTOSIDE-BINDING LECTINS;THURINGIENSIS SUBSP AIZAWAI;WHEAT-GERM-AGGLUTININ;BACILLUS-THURINGIENSIS;PERITROPHIC MEMBRANE;MUSCLE LECTIN;PROTEIN;MIDGUT;LARVAE;LOCALIZATION
    日期: 2009
    上传时间: 2010-06-29 19:28:53 (UTC+8)
    出版者: 中央大學
    摘要: BACKGROUND: Previous studies showed that mammalian galectin-1 (GAL1) could interact with chitosan or chitin, one component of the peritrophic membrane (PM). This finding suggests that the PM could be a target of GAL1, which prompted the authors to explore the effect of GAL1 on larval growth and its potential mechanism. RESULTS: The development of Plutella xylostella (L.) larvae was significantly disturbed after they were fed recombinant GAL1. The histochemical structure and immunostaining pattern suggested that GAL1 treatment resulted in dose- and time-dependent disruption of the microvilli and abnormalities in these epithelial cells. Ultrastructural studies showed that the PM was not present in the midgut of GAL1-treated insects; instead, numerous bacteria were found in the lumen area. These results indicate that the protective function of the PM was disrupted by GAL1 treatment. Moreover, in vitro data showed that GAL1 interacts with chitosan/chitin in a dose-dependent manner, and also specifically binds to the PM in vitro. CONCLUSION: In view of the fact that the carbohydrate recognition domain of GAL1 recognises the structural motif N-acetyl lactosamine (Gal beta 1-4 GIcNAc), which is similar to that of chitin (beta-1, 4 N-acetyl-D-glucosamine), it is proposed that the insecticidal mechanism of GAL1 involves direct binding with chitin to interfere with the structure of the PM. () 2009 Society of Chemical Industry
    關聯: PEST MANAGEMENT SCIENCE
    显示于类别:[生命科學研究所 ] 期刊論文

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