Translational Efficiency of Redundant ACG Initiator Codons Is Enhanced by a Favorable Sequence Context and Remedial Initiation

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Title:	Translational Efficiency of Redundant ACG Initiator Codons Is Enhanced by a Favorable Sequence Context and Remedial Initiation
Authors:	Chen,SJ;Ko,CY;Yen,CW;Wang,CC
Contributors:	生命科學研究所
Keywords:	TRANSFER-RNA SYNTHETASE;NON-AUG INITIATION;SACCHAROMYCES-CEREVISIAE;STRUCTURAL FEATURES;RIBOSOMAL-SUBUNIT;GENE ENCODES;IN-VIVO;YEAST;MITOCHONDRIAL;PROTEIN
Date:	2009
Issue Date:	2010-06-29 19:28:54 (UTC+8)
Publisher:	中央大學
Abstract:	Earlier studies showed that the redundancy of ACG initiation codons enhanced the efficiency of translation initiation by 3- to 6-fold. Evidence presented here shows that this "redundancy effect" can be attributed to a favorable sequence context and, to a lesser extent, remedial initiation. In the case of redundant ACG initiator codons, the second ACG not only acts as a remedial initiation site for scanning ribosomes that skip the first ACG but also enhances the activity of the preceding initiator by providing a preferable "A" at its relative +4 position. Hence, non-successive ACG codons can be as effective as successive ACG codons in initiation, if positioned within a similar context. In contrast, redundant GUG initiation codons (GUG/GUG) bear an unfavorable "G" nucleotide at both the +4 and -3 positions relative to the first and second GUGs, respectively, such that redundant GUG codons act more poorly as translation initiation sites than does a single GUG with a favorable "A" nucleotide in the +4 position (similar to 2.5-fold). Thus, the sequence context plays a much more important role than remedial initiation in modulating the efficiency of translational initiation from redundant non-AUG codons.
Relation:	JOURNAL OF BIOLOGICAL CHEMISTRY
Appears in Collections:	[Graduate Institute of Life Science] journal & Dissertation

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