English  |  正體中文  |  简体中文  |  Items with full text/Total items : 66984/66984 (100%)
Visitors : 23024174      Online Users : 161
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version


    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/28058


    Title: Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor
    Authors: Huang,JC;Chang,YF;Chen,KH;Su,LC;Lee,CW;Chen,CC;Chen,YMA;Chou,C
    Contributors: 光電科學研究所
    Keywords: LINKED-IMMUNOSORBENT-ASSAY;REVERSE-TRANSCRIPTASE PCR;MONOCLONAL-ANTIBODY;SENSITIVE DETECTION;STOOL SPECIMENS;SPIKE PROTEIN;THROAT WASH;ANTIGEN;AMPLIFICATION;NANOPARTICLES
    Date: 2009
    Issue Date: 2010-06-29 19:41:03 (UTC+8)
    Publisher: 中央大學
    Abstract: In order to enhance the sensitivity of conventional immunoassay technology for the detection of SARS coronavirus (SARS-CoV) nucleocapsid protein (N protein), we developed a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor that combines sandwich immunoassay with the LSP technique. Experimentally, a linear relationship between the fluorescence signal and the concentration of recombinant SARS-CoV N (GST-N) protein in buffer solution could be observed from 0.1 pg/mL to 1 ng/mL In addition, the concentration of GST-N protein in diluted serum across a similar range could also be measured. The correlation coefficients (linear scale) for these two measurements were 0.9469 and 0.9624, respectively. In comparison with conventional enzyme linked immunosorbent assay (ELISA), the detection limit of the LSPCF fiber-optic biosensor for the GST-N protein was improved at least 10(4)-fold using the same monoclonal antibodies. Therefore, the LSPCF fiber-optic biosensor shows an ability to detect very low concentration (similar to 1 pg/mL) of SARS-CoV N protein in serum. The biosensor should help with the early diagnosis of SARS infection. (C) 2009 Elsevier B.V. All rights reserved.
    Relation: BIOSENSORS & BIOELECTRONICS
    Appears in Collections:[光電科學研究所] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML1019View/Open


    All items in NCUIR are protected by copyright, with all rights reserved.

    社群 sharing

    ::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback  - 隱私權政策聲明