併同去除碳、氮、磷之生物處理程序已是現今生物處理之主流，但是併同去除的系統中存在兩個問題，一是磷菌與硝化菌所需的SRT長短不同之衝突；一為磷菌與脫硝菌在碳源上的競爭問題。本論文利用TNCU-I程序特性，好氧槽上的RBC可以增殖需較長SRT的硝化菌，解決磷菌與硝化菌在SRT上的衝突，另外，在TNCU-I程序中也發現有脫硝除磷菌的存在，藉由促使脫硝除磷菌大量的增殖，磷菌與脫硝菌在碳源上的競爭也得以解決。此外，由於TNCU-I程序上有一組RBC，因此，對於突變負荷有較高的忍受能力。 因此，本研究將TNCU-I程序操作於SRT=10天，變動進流基質濃度以觀察TNCU-I程序對突變負荷的忍受能力，並設計一系列之批次實驗，以TNCU-I程序之活性污泥探討在不同內部碳源濃度的條件下，有不同濃度外部殘留碳源存在之缺氧/好氧的狀態下，微生物之缺氧脫硝釋磷/脫硝攝磷及好氧釋磷/攝磷之反應行為。 由實驗結果顯示，TNCU-I程序在各種不同的變動負荷下，對COD都能維持在相當高的去除率，而N、P的去除，雖沒有如去除有機碳那麼穩定，不過也都能快速地恢復穩定，此點證實TNCU-I程序對變動負荷確實具有較高的處理穩定性。 在不同內部碳源濃度的條件下，有外部殘留碳源存在時，在缺氧/好氧的狀態下，由微生物之缺氧脫硝釋磷/脫硝攝磷及好氧釋磷/攝磷之批次實驗，可看出就相同內部碳源及外部殘留碳源的情況下，利用O2當電子接受者的比攝磷率比利用NO3當電子接受者的比攝磷率高。且攝磷反應受電子接受者的影響比受外部殘留COD濃度高低的影響還明顯。整體而言，內部碳源濃度越高，外部殘留COD濃度越低，越傾向於攝磷。 在相同的內部碳源濃度情況下，外部殘留碳源濃度越高，其比脫硝率就越高。且在相同的外部殘留碳源濃度情況下，內部碳源濃度越高，其比脫硝率也越高。整體而言，不論內部碳源越高或外部碳源越高，其比脫硝率都會越高。 The biological nutrient removal (BNR) processes are the main processes treating the municipal wastewater. However, there are two problems in the most BNR processes. One problem is the conflict of SRT between phosphate accumulating organisms (PAO) and nitrifier. The other is the competition of carbon source between de-phosphate and denitrogen. Thus, our group developed a novel process (TNCU-I process) that added a RBC in the aerobic tank of the traditional A2O process. The sludge retention time of the nitrifier can be extended on the RBC to reduce the sludge retention time of activated sludge to solve the conflict the SRT between PAO and nitrifier. Consequently, the anoxic zone of the activated sludge can increased to enhance the present of denitrifying phosphate accumulating organisms (DNPAO) to solve the competition of carbon source between de-phosphate and denitrogen. Additionally, the tolerance ability of shock loading could be increased due to the present of RBC. In light of above discussion, this study investigated the influence of shock loading on the performance of TNCU-I. Additionally, the anoxic/aerobic phosph-ate uptake/release reactions under various initial COD and residue COD conditions of TNCU-I process were also performed by a series of batch experiments. The results revealed that the TNCU-I process showed excellent performance of COD removal under various shock loading. Although the performance of nitrogen and phosphate removal was not so good as COD, it could return to the steady state rapidly under various shock loading. This improved that the TNCU-I process showed good stability of treating shock loading influent. The results of batch experiments showed that the potential of phosphate uptake was dynamic. It increased with the initial COD increased, the initial PHA increased, and the residue COD decreased. Furthermore, the phosphate uptake/release influenced by electron acceptor (oxygen of nitrate) was more significantly than residue COD concentrations. Additionally, the specific denitrification rate increased with the initial COD and the residue COD increased.