本研究將實驗分成三大部分，一為水溶性羧甲基幾丁聚醣(O- carboxymethyl-chitosan，OCC)之合成；二為陰離子型WPU/OCC系統及陽離子型WPU/幾丁聚醣快速交聯製程；三則為陽離子水性PU摻合幾丁聚醣之溼-乾製程，分別藉由DMA、SEM、拉力、抑菌等測試來探討其膜材物性及抑菌能力分析。 羧甲基幾丁聚醣/陰離子型WPU系統是利用化學修飾的方式進行水溶性羧甲基幾丁聚醣(OCC)之合成，即在幾丁聚醣第6個碳上之氫氧根上改接羧甲基。而改質過後之羧甲基幾丁聚醣具有良好的親水性，可以與陰離子型水性PU進行混摻。進而利用戊二醛、EGDE分別與混摻基材交聯形成一半互穿型網狀結構體(semi-IPN)來增加膜材的穩定性；再和陽離子型WPU與幾丁聚醣利用高濃度戊二醛交聯膜材作以比較；另一系統則將陽離子型WPU與幾丁聚醣以濕-乾製程法來備製膜材，以期得到一無交聯劑存在的素材。 在物性方面，發現兩款陰離子型WPU與羧甲基幾丁聚醣(OCC)混摻之薄膜，皆比原WPU有較佳熱穩定性及抗張強度，混摻性則以酯型WPU與OCC較好；而經戊二醛及EGDE交聯後之膜材，穩定性皆提升；斷裂點延伸率則以醚型WPU/OCC膜材隨EGDE交聯濃度增加而提升。陽離子型WPU與幾丁聚醣快速交聯之膜材物性則與前系統類似，不過幾丁聚醣的摻入其熱穩定性質的改變更明顯。而陽離子型WPU與幾丁聚醣濕-乾製程之混摻膜材，不但熱穩定性較原PU佳，在抗張強度上也遠大於兩混摻高分子，且仍具柔軟性。在抑菌方面，無論是陰離子型WPU與OCC之混摻膜材抑或是陽離子型 WPU與幾丁聚醣混摻膜材，皆隨OCC及幾丁聚醣的摻入，提升了原WPU對於E. coli的抑菌能力。 Three research subjects were studied in this paper; first: Synthesis of O-Carboxymethyl Chitosan (OCC), second: blending of OCC with polyurethane anionomer (WPU-anionomer) and blending of chitosan with polyurethane cationomer (WPU-cationomer) by high concentration of crosslinking agent such as glutaradehyde; third: WPU-cationomer /chitosan membrane prepared by wet process. Morphology and mechanical properties of membranes were studied by Scanning Electron Microscopy (SEM), Dynamic Machine Analysis (DMA) and Instron, antimicrobial ability of membranes was investigated by antibacterial test against E. coli. OCC was prepared by chemical modify the OH- group in the sixth carbon of chitosan to CH2COO-. Owing to the good hydrophilic property of CH2COO- group, OCC can blend with polyurethane anionomer easily. In order to increase the thermal stability of the blend membranes, glutaraldehyde or Ethylene, Polyethlen Glycol Diglycidyl Ether (EGDE) was used as crosslinking agent to form semi-IPN. The physical properties of WPU-anionomer/OCC semi-IPN were compared with that of WPU-cationomer/chitosan crosslinked by high concentration glutaradehyde. In order to have a thermal stable membrane without any crosslinking agent, we also prepared a WPU-cationomer/chitosan membrane by wet process. WPU-anionomer/OCC blend membranes have better thermal stability and mechanical properties than that of WPU-anionomer. The ultimate elongation of ether type WPU-anionomer/OCC membrane increase with increasing the concentration of crosslinking agent EGDE. The WPU-cationomer/chitosan blend membrane proparaed by wet process maintain their original flexibility but have better thermal stability and mechanical strength than that of WPU-cstionomer. Antibacterial test demonstrate that WPU-cationomer blending with chitosan has better antimicrobial capability against E. coli.
