| 摘要: | 人類的平均壽命越來越高，也越來越多人開始注重身體的保養， 目前已有證據顯示自由基與人體疾病的產生有相當程度的關連。有研 究指出動脈粥狀硬化（Witzum,1994），呼吸道疾病（Cross et al.,1994），神經性退化疾病（Jenner,1994），發炎（Grisham,1994）， 癌症（Beckman and Ames,1997），老化（Beckman and Ames,1997）等 疾病的發生都與自由基有關。 樟芝又稱牛樟芝或牛樟菇，只生長在台灣特有的牛樟樹內，屬於 無褶菌目、多孔菌科。在民間盛傳樟芝的功效頗多，而在有關抗氧化 的研究中，Song and Yen（2002）之研究指出樟芝發酵液與發酵菌絲 之萃取物在實驗中具有清除超氧陰離子及抑制脂質過氧化之能力；而 在蔡雁暉（2002）之研究亦明確指出樟芝多醣體具有良好抗氧化能力。 本研究利用氧自由基吸收能力測試（Oxygen-Radical Absorbance Capacity assay;ORAC assay）?抗脂質過氧化測試（Anti-Lipid Peroxidiant Capacity assay;ALPC assay）以及總抗氧化（Trolox Equivalent Antioxidant Capacity;TEAC）測定來探討通氣量對於樟芝醱 酵生產抗氧化物質之影響，並且利用工程的技術使抗氧化物質提升。 由實驗中可以得知以下幾點結果：（1）抗氧化物質與多醣體之生 產是以一次代謝為主。（2）抗氧化能力及多醣體與通氣量 (0.3vvm~1.0vvm)成正比關係。（3）粗多醣在抗脂質過氧化能力與氧 自由基吸收能力中扮演著重要之角色。（4）在各個通氣量 (0.3vvm~1.0vvm) 中，1.0vvm 是抗氧化物質生產之最佳條件。 The human average life span is higher and higher, more and more people too begin maintenance to pay attention to body , have evidence show free radical and human production is it connect with to imprisonning to no small extent of disease already at present. Study and point out scleratheroma (Witzum ,1994),Breathing problem (Cross et al., 1994),(Jenner of disease that the nerve degradates ,1994),Inflammation (Grisham ,1994),Cancer (Beckman and Ames ,1997),Wear out (Beckman and Ames ,1997)Wait for the emergence of the disease to all relate to free radical. The camphor tree sesame has another name called ox's camphor tree sesame or ox camphor tree mushroom, only grow in peculiar ox camphor tree of Taiwan, have no fungus item of pleat , porous fungus department. A lot of in folk efficiency of widelying spread the camphor tree sesame, in relevant anti-oxidant research, Song and Yen ( 2002) Research point out not having the fermented the sesames liquid of camphor tree it have not lasting oxygen anion not ultra and not suppressing fat the qualities not to pass ability that oxidize of with extraction thing of hypha not fermented on experiment ; Also point out clearly in the research of Cai YanHui (2002 ) that many candy body of camphor tree sesame has good anti-oxidant ability . This research utilizes oxygen free radical to absorb aptitude tests (Oxygen-Radical Absorbance Capacity assay; ORAC assay), resist fat quality is it oxidize testing to pass (Anti-Lipid Peroxidiant Capacity assay; ALPC assay), and always anti-oxidant (Trolox Equivalent Antioxidant Capacity; TEAC) determine and probe into the keeping in touch with amount to set aside the influence that the ferment produces the anti-oxidant material on the camphor tree sesame, and utilize technology of the project to make the anti-oxidant material improve. Can learn some following results from the experiment : ( 1) The production of anti-oxidant material and many candy body is to rely mainly on supersession. ( 2) Anti-oxidant ability and much candy body and relation in direct radio of the keeping in touch with amount (0.3vvm resists 1.0vvm ). ( 3) In resist thick many candy fat quality is it oxidize. ( 4) In each keeping in touch with amount (0.3vvm is in 1.0vvm ), 1.0vvm is a optimum condition of the anti-oxidant material production. |
