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    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/3869

    Title: 幾丁聚醣接枝半乳糖簇之材料性質及其肝靶向性研究;Meterials characters and liver targer to study of chitosan graft galactose
    Authors: 黃美鳳;Mei-Feng Huang
    Contributors: 化學工程與材料工程研究所
    Keywords: 半乳糖;幾丁聚醣;chitosan;galactose
    Date: 2005-07-11
    Issue Date: 2009-09-21 12:24:42 (UTC+8)
    Publisher: 國立中央大學圖書館
    Abstract: 本實驗研究,以幾丁聚醣和半乳糖醛酸以共價聯接形成一個接枝半乳糖基的幾丁聚醣薄膜和奈米粒子;另以L-lysine作為中間體,以L-lysine上的胺基和半乳糖醛酸上羧基之凝結回應合成結束簇狀(cluster)接枝半乳糖基之幾丁聚醣薄膜和奈米粒子;分別針對其物化性(拉力、抗菌等等)、Gal-1吸附能力、CLSM(Confocal scanning Microscope)觀察之肝臟細胞標靶測試分析。 薄膜實驗中顯示,當幾丁聚醣接枝半乳糖醛酸後,明顯的可以提升膜材對於E.coil 的抑菌能力,其因半乳糖基為清水基團可增加抗菌能力;接枝半乳糖基之幾丁聚醣薄膜較原始未接枝之幾丁聚醣薄膜明顯具有對Gal-1較佳之結合效能;肝細胞貼附型態模式也都因半乳糖基與肝細胞表面受體ASGP-R之間的半乳糖基特化辨識(galactose-specific recognition)主導了肝細胞在材料表面之貼附型態。 奈米粒子實驗其奈米粒子約300~500nm,且相對的zeta-potential,有接枝lysine之幾丁聚醣奈米粒子或簇狀接枝半乳糖基團之幾丁聚醣奈米粒子均明顯具有較高;而奈米粒子方面,其型態受了三聚磷酸之濃度及pH值之控制;最後以奈米粒子在固定時間點以CLSM(Confocal scanning Microscope)觀察之肝細胞標靶試驗,發現標的試驗之時間越長,螢光越明顯,且觀察到簇狀接枝半乳糖基團之幾丁聚醣奈米粒子都比幾丁聚醣及單點接枝半乳糖基之幾丁聚醣奈米粒子標的至肝細胞的數目明顯為多,作為奈米粒子標的至肝臟細胞之效率評估及佐證。 In this study, the synthesis and characterization of a novel galactosyl chitosan are reported. The novel galactosyl chitosan was prepared by covalent coupling of lactobionic acid with chitosan(gal-chit) ; and galactosyl chitosan was prepared by introducing a L-lysine spacer to chitosan,followed by covalent coupling of lactobionic acid with the lysine spacer on chitosan(gal-lys-chit) ,and witch maked into membranes and nano-particles experiment. Morphology and mechanical properties of membranes were studied by FTIR analysis, X-ray diffraction, swelling study, antibacterial test, bind galectin-1 and cell train. Nano-particles were studied by FTIR analysis, X-ray diffraction ,nano-particles sizes , zeta potential and target to liver cells. In the film experiment, improve the ability to resist fungus of the gal-chit and gal-lys-chit membrane materials, because the lactobionic acid is base group of the hydrophile; and the ability to effectively bind galectin-1(Gal-1); galactose and surface receptor of liver cell can lead sticking to the growing type attitude on the material of liver cell. In the nano-particles experiment, gal-chit,gal-lys,gal-lys-chit of the value are relatively high of the nano-paticles and zeta potential ,because three dimensional obstacle of the galactose of ring structure will influence effort of the electric charge between positive electricity amine base on the chitosan and negative electricity of TPP.The nano-particles form is affected by PH value and concentration of TPP.Nano-particles at some of set time observed to target cells of liver, the longer time is gal-lys-chit NPs has more apparent result than gal-chit NPs. Can be as efficiency assessing and evidence of enduring liver cell that the rice particle marks.
    Appears in Collections:[化學工程與材料工程研究所] 博碩士論文

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