Interaction of Tea Catechin (-)-Epigallocatechin Gallate with Lipid Bilayers

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题名:	Interaction of Tea Catechin (-)-Epigallocatechin Gallate with Lipid Bilayers
作者:	Sun,Y;Hung,WC;Chen,FY;Lee,CC;Huang,HW
贡献者:	物理研究所
关键词:	ANTIMICROBIAL PEPTIDES;PORE FORMATION;DIPHYTANOYL PHOSPHATIDYLCHOLINE;SPONTANEOUS-CURVATURE;GREEN TEA;MEMBRANE;VESICLES;MODEL;MECHANISM;CURCUMIN
日期:	2009
上传时间:	2010-07-08 13:59:59 (UTC+8)
出版者:	中央大學
摘要:	A major component of green tea extracts, catechin(-)-Epigallocatechin gal late (EGCg), has been reported to be biologically active and interacting with membranes. A recent study reported drastic effects of EGCg on giant unilamellar vesicles (GUVs). In particular, EGCg above 30 mu M caused GUVs to burst. Here we investigated the effect of EGCg on single GUVs at lower concentrations, believing that its molecular mechanism would be more clearly revealed. We used the micropipette aspiration method, by which the changes of surface area and volume of a GUV could be measured as a result of interaction with EGCg. We also used x-ray diffraction to measure the membrane thinning effect by EGCg. To understand the property of EGCg, we compared its effect with other membrane-active molecules, including pore-forming peptide magainin, the turmeric (curry) extract curcumin, and detergent Triton X100. We found the effect of EGCg somewhat unique. Although EGCg readily binds to lipid bilayers, its membrane area expansion effect is one order of magnitude smaller than curcumin. EGCg also solubilizes lipid molecules from lipid bilayers without forming pores, but its effect is different from that of Triton X100.
關聯:	BIOPHYSICAL JOURNAL
显示于类别:	[物理研究所] 期刊論文

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