| 摘要: | 絲狀真菌是指真菌的一種型態，是以絲狀菌絲構成的。真菌對人類而言是一種相當重要的資源，它的衍生功能相當的多，像是生長過程中所產的代謝物，在我們的醫療、農業及工業等方面皆佔有很重要的地位。目前學術界對於真菌已有相當的研究，其中一種就是將真菌行固定化，而固定化菌體就是希望能達到回收再利用的目的。菌體經由固定化後可以節省菌體生長的時間並減少營養源的消耗，且可以重複利用菌體將所產生的代謝物產量給提高，但一般利用不織布或棉布的固定化方法會影響到菌體生長的情形，菌體的型態改變之後勢必會影響到菌體的代謝情況。 本研究以屬於絲狀真菌的裂褶菌(Schizophyllum commune)為研究的主軸，先利用氧化鐵磁性粒子(Fe3O4)來回收菌體，以達固定化的目地，並且利用Fed-batch 1次的方式來探討其對代謝物多醣產量的影響，並與一般的固定化方式(不織布)比較。 結果顯示，氧化鐵磁性粒子(Fe3O4)可以成功用於回收裂褶菌，並且實行1次Fed-batch實驗後，發現裂褶菌多醣產量較batch高2.4倍，產率也提升了3倍左右，而一般的固定化方法卻未能有效提高產物含量及產率。 另外在行Fed-batch時我們減少氮源的含量，發現多醣量可以較batch的多醣量高出約3倍，效果比未將氮源減少的2.4倍還好，單位菌體對產物的轉化率Yp/x也可提升1.7倍，而一般的固定化方法卻無此效果。 Fe3O4之所以能回收裂褶菌，是因為其會黏附在菌體的表面，本研究也簡單利用定性的方法來探討黏附的行為，並以其它絲狀真菌Aspergillus niger及Penicillim brevicompactum來驗證。 為了改善Fe3O4無法從菌體上脫落的缺點，本研究以溫度敏感性磁性粒子(PolyNIPAAm-Fe3O4)來進行裂褶菌的回收，此磁性粒子擁有LCST(Lower Critical Solution Temperature)的性質。結果發現在室溫25℃時PolyNIPAAm-Fe3O4並不會有吸附菌體的現象，而把環境溫度提升到50℃時才會有吸附的行為。 Filamentous fungi refers to a type of fungus, is composed of filamentous hyphae. Fungi were very important resources in terms of human, and its considerable multi-functional derivatives, such as the growth process of the metabolites produced in our health care, agriculture and industries are a very important position. At present, the fungus has been considerable academic research, which is immobilizing fungi; immobilized cell is to achieve the purpose of recycling. Immobilized cell by cell growth can save time and reduce the consumption of nutrients, and can re-use will be produced by fungal metabolites to increase the yield, but generally the use of fixed method of non-woven or cotton cloth will affect the fungi Growth of the case, the morphology changed after the cell is bound to affect the metabolism of fungi. In this study, belonging to the filamentous fungi Schizophyllum commune for the study of the spindle, the first use of magnetic particles (Fe3O4) to recover the cell to achieve the purposes immobilized, and the use of Fed-batch 1 time the way to explore its metabolites yield of polysaccharide and with the general approach of the fixed (non-woven) to compare. The results showed that magnetic particles (Fe3O4) can be successfully used for recovery of Schizophyllum commune, and the implementation of 1 Fed-batch experiments, we found that production of Schizophyllum commune polysaccharide than 2.4 times the high batch production rate increased about 3 times , and immobilized in general are not effective methods to improve content and product yield. In addition, among the Fed-batch, we reduce the nitrogen source, the amount of polysaccharide can be found in the polysaccharide than the batch volume about 3 times than not reduce the nitrogen 2.4 times better, the product of unit cell of the conversion rate of Yp / x can also be upgraded to 1.7 times, and general methods of no fixed effect. Fe3O4 reason Schizophyllum commune recovery because of its will in the cell surface adhesion, the simple use of qualitative research methods to study the adhesion behavior, and other filamentous fungi Aspergillus niger and Penicillim brevicompactum to verify. In order to improve on Fe3O4 fungal shedding from the shortcomings of this study, the temperature sensitivity of magnetic particles (PolyNIPAAm-Fe3O4) to carry out the recovery of Schizophyllum commune, the magnetic particles have a LCST (Lower Critical Solution Temperature) in nature. The results showed that at room temperature at 25 ℃ PolyNIPAAm-Fe3O4 does not have the phenomenon of bacterial adsorption, and the ambient temperature to 50 ℃ when the act will be adsorbed. |
