探討稻殼酸水解液中有害物質的移除對Pichia stipitis BCRC 21775生產乙醇之影響 ;Effect of detoxification methods of dilute-acid hydrolyzates for fermentation by Pichia stipitis BCRC 21775 on ethanol production

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Title:	探討稻殼酸水解液中有害物質的移除對Pichia stipitis BCRC 21775生產乙醇之影響;Effect of detoxification methods of dilute-acid hydrolyzates for fermentation by Pichia stipitis BCRC 21775 on ethanol production
Authors:	劉家宏;Chia-Hung Liou
Contributors:	化學工程與材料工程研究所
Keywords:	酵素;生質酒精;稀酸水解;laccase;Agaricus blazei Murrill;Pichia stipitis
Date:	2008-07-09
Issue Date:	2009-09-21 12:30:40 (UTC+8)
Publisher:	國立中央大學圖書館
Abstract:	最近幾年，由於現代工業的發展，使得化石燃料快速的消耗，造成能源價格節節高漲。因此，目前全世界各國都在積極進行新能源之研究開發，其中，生質能( biomass energy )因其原料取自大自然，且能生生不息地被循環使用，故成為最受矚目的替代能源之一。纖維素是一種生質能的原料，將纖維素水解後，生成容易發酵的單醣，再經微生物發酵後，所產生的酒精即為生質酒精。一般纖維素水解的化學方法包含了稀酸水解和濃酸水解。本實驗中採用稀酸水解法，稀酸水解雖然反應速度快，但因為不具選擇性，會有大量副產物的生成，如:乙酸(Acetic acid)、Furfural、HMF、LDP…等等，使得酒精產率過低而不具經濟價值。在本研究中，選擇酸水解條件為1% H2SO4 、酸水解時間為60分鐘、固液比為10%、121∘C、1.2atm，所產生的酸水解液，則利用Agaricus blazei Murrill 所產的laccase酵素及百貴LC-A1活性碳移除其中的有害物質。利用添加laccase酵素可移除酸水解液中53%的LDP，而添加百貴LC-A1活性碳則可100%移除酸水解液中的乙酸。本研究中，以攪拌式反應器來進行液態發酵培養酵母菌來產生酒精，所使用的菌株為 Pichia stipitis BCRC 21775。在實驗中，我們探討添加不同活性單位的laccase 酵素及活性碳，對Pichia stipitis BCRC 21775產生酒精的影響。實驗結果發現如下: (1) 在本研究稀酸水解條件下，LDP ( Lignin degradation products ) 為抑制酒精產率的主要物質。 (2) 在以不同來源的laccase降解LDP實驗中，可以發現由A. blazei 所產的laccase其降解LDP能力高於傳統T. versicolor所產laccase，且經A. blazei laccase處理的酸水解液的酒精產量也優於傳統 T. versicolor laccase 處理的酸水解液。 (3) 經處理的酸水解液其酒精產量、菌體生長都比未處理的高，且發酵時間也比未處理的短，其中以添加10000U/L laccase酵素其酒精產量(5.62g/L→10.71g/L)、比生長速率μ (0.006→0.013)為未處理的兩倍，菌體生長為未處理的1.85倍。 During hydrolysis of lignocellulosic materials a wide range of compounds which are inhibitory to microorganisms are formed or released. Based on their origin the inhibitors are usually divided in three major groups: weak acids, furan derivatives, and phenolic compounds. These compounds limit effcient utilisation of the hydrolysates for ethanol production by fermentation. This research discusses the generation of detoxification methods , and the effect of these on fermentation yield and productivity. Choosing acid-hydrolysates conditions , reaction time was sixty - minuite and solid /liquid ratio was ten percent , and the reducing sugar conversion was 83% . Then use enzyme laccase and activated charcoal removal of toxic compounds in the acid-hydrolysates , the ethanol yield and specific growth rate ehanced two times , the biomass yield ehanced 1.85 times .
Appears in Collections:	[National Central University Department of Chemical & Materials Engineering] Electronic Thesis & Dissertation

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