中大機構典藏-NCU Institutional Repository-提供博碩士論文、考古題、期刊論文、研究計畫等下載:Item 987654321/4113
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 70588/70588 (100%)
造訪人次 : 23215156      線上人數 : 532
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋


    請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/4113


    題名: 探討添加有機酸對Penicillium Brevicompactum在液態發酵中生產Mycophenolic acid之影響;Effects of organic acids on Mycophenolic acid product of Penicillium Brevicompactum in submerged fermentation
    作者: 李慶彬;Ching-pin Li
    貢獻者: 化學工程與材料工程研究所
    關鍵詞: 麥考酚酸;有機酸;短密青黴菌;organic acids;Penicillium brevicompactum;Mycophenolic acid
    日期: 2009-07-07
    上傳時間: 2009-09-21 12:31:22 (UTC+8)
    出版者: 國立中央大學圖書館
    摘要: Mycophenolic acid (MPA) 具有抗牛皮癬、抗真菌、抗細菌及抗病毒的能力,且已被證實為Inositol MonoPhosphate DeHydrogenase (IMPDH) 之非競爭型免疫抑制劑,針對淋巴細胞之合成路徑進行抑制而達到降低免疫反應的發生,因此在臨床上被用來預防器官移植所產生的排斥現象。實驗上利用微生物Penicillium brevicompactum進行深層液態發酵 (submerged fermentation) 來生產MPA。 本研究探討添加有機酸對P. brevicompactum生長及生產MPA的影響,其中發現於三羧酸循環中所代謝之有機酸如檸檬酸及琥珀酸能影響P. brevicompactum生長及產物代謝。在添加檸檬酸的情況下能促進菌體生長及加速碳源的消耗,提升菌體比生長速率 (μ) ,可達0.0189,為控制組的1.56倍,且能縮短發酵所需時間約25 hr,產率 (productivity) 提升為控制組的1.3倍;而添加琥珀酸則能提高MPA的產量及提升單位菌體對產物的轉化率 (Yp/x) ,以添加1 g/L達最高MPA產量0.536 g/L,約提升為控制組的1.27倍,單位菌體對產物的轉化率為60.06 mg/g,約為控制組1.33倍。 Mycophenolic acid (MPA) has been to be antipsoriasis, antifungal, antibacterial and antiviral. It was demonstrated that MPA is a non-competitive immunosuppressive agent for Inositol MonoPhosphate DeHydrogenase (IMPDH) by inhibiting de novo purine biosynthesis of lymphocytes. Therefore, MPA is used clinically to prevent rejection of transplanted organs. In this study, Penicillium brevicompactum was used to product MPA in submerged fermentation. Effects of various organic acids on cell growth and production of a useful bioactive metabolite, MPA, by submerged cultivation of Penicillium brevicompactum were investigated. The organic acid such as citric acid and succinic acid, which present in the tricarboxylic acid cycle, were found to affect the cell growth and its metabolism. Addition of citric acid stimulated cell growth, increased consumption rate of carbon source and reduced fermentation time about 25 hr.The specific growth rate and productivity were 1.56 and 1.3 times than that of control-level, respectively. In addition, addition of succinic acid under 1 g/L concentration increased MPA production to 0.536 g/L, which was 1.27 times than that of control-level. Meanwhile the Yp/x increased 1.33 times than that of control-level.
    顯示於類別:[化學工程與材料工程研究所] 博碩士論文

    文件中的檔案:

    檔案 大小格式瀏覽次數


    在NCUIR中所有的資料項目都受到原著作權保護.

    社群 sharing

    ::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋  - 隱私權政策聲明