工作目標:完成乙型受體素(β-agonist)簡易層析免疫檢驗試劑之開發: 1. 腹水生產及單多株抗體純化及測定方法確認。 2. 完成Clenbuterol半抗原衍生物之合成。 3. 完成半抗原衍生物-大分子蛋白結合體製備。 4. 完成進行簡易免疫層析試片產品開發及雛形套組生產及品管方法建立。 5. 完成檢驗套組試量產及品質測試結果驗證。預期效益: 1. 完成抗單多株抗體純化50 mg。 2. 完成半抗原衍生物之大量合成及50 mg。 3. 完成半抗原衍生物之蛋白質結合體50 mg。 4. 完成產品開發及雛形套組生產及品管方法建立。 5. 完成小量田間測試。 Specific aims: Development of immunochromatographic rapid test model for residues of beta-agonist in animal products. Important works in this year: 1. Find out proper condition of production and purification for anti- beta-agonist monoclonal/polyclonal antibody. 2. Preparation of derivatives of beta-agonists. 3. Preparation of conjugate complexes of to different carrier proteins. 4. Establish immunochromatographic rapid test system. 5. Farm trial in small scale. Expected results: 1. Finish 50 mg of purification of monoclonal antibody. 2. Finish 50 mg of purification of beta-agonist derivatives-protein conjugation. 3. Finish establishment of immunochromatographic rapid test model. 4. Finish the farm trial in small scale. 研究期間:9401 ~ 9412