低分子量熱休克蛋白質是植物於熱逆境下累積量及種類最多的蛋白質,目前由於溫室效應的影響,地球暖化,由於人類的過度開發大量喪失遺傳多樣性,且多生活在高山環境下無可避免地將面對高溫的抗逆,因此目前急需了解植物的抗熱分子遺傳機制,鑑定出可能參與的抗熱蛋白,以應付急速的溫度變異所造成的傷害,本研究將針對台灣黃苑屬植物不同類型的小分子量熱休克蛋白基因家族基因成員,探討其分子演化模式和表現形式,分別偵測細胞質內第一型葉綠體型小分子量熱休克蛋白基因(94年)、葉綠體型小分子量熱休克蛋白基因(95年)和粒線體型小分子量熱休克蛋白基因(96年)之遺傳多樣性,以及影響分子演化的可能機制,為了解可能的抗熱機制,在演化分析中也將檢測各型小分子量熱休克蛋白基因所遭受的天擇模式。 The small heat shock protein (sHSP) is one subset of the multichaperone network and the most abundant one in plants. In the past, due to extensive exploration by human, the genetic diversity has reduced dramatically. It is important to understand the mechanism of small heat shock proteins, which are the main component of anti-heat pathway. It is good for constructing the relative genes of anti-heat mechanism to develop competitiveness characters and lines of lily. This study will focus on molecular evolution and expression patterns of the genes of the small heat shock proteins in Arabis in Taiwan. Between the small heat shock proteins family, intraspecific genetic variation in cytosolic I type (1st year), chloroplas type (2nd year) and mitochondrial type (3rd year) will be examined. Natural selection hypothesis will be tested and modes of natural selection will be identified. 研究期間:9401 ~ 9412