近年來，由於全球石化能源逐漸耗竭及原油價格高漲，尋找永續再生性的新能源已成為一個國家賴以生存及發展經濟所必須努力的目標。厭氧產氫、甲烷，生質乙醇（bioethanol）及生質柴油（biodiesel）等綠色能源的發展受到重視。 本論文以傳統式包埋Saccharomyces cerevisiae 作為控制組，設計立即式包埋法( in-situ immobilization )與可控式包埋法 (controllable immobilization)進行比較，而立即式包埋法可以改善傳統式包埋結構、質傳問題，可控式與立即式的差異在於，多了菌體釋出步驟，使得菌體利用基質的效率更佳。 以100 g/L、200 g/L的葡萄糖，探討立即式與可控式於乙醇醱酵之可行性，結果發現，Saccharomyces cerevisiae 於100 g/L葡萄糖下，立即式、可控式包埋法較傳統式包埋之產率多了約1.35倍，於200 g/L葡萄糖下，在基質與乙醇濃度的抑制下仍有良好的產率，立即式與可控式分別高於傳統包埋1.28與1.15倍，相同條件下，立即式包埋的菌體活性幾乎維持95 %之間，而傳統式會隨之遞減至93 %，導致乙醇總產量與轉化率不及於立即式。 Ethanol use as a fuel additive or directly as a fuel source has grown in popularity due to governmental regulations and in some cases economic incentives based on environmental concerns as well as a desire to reduce oil dependency. There are two-type designs which in-situ immobilization and controllable immobilization respectively are to improve the mass transfer problems of traditional immobilization and comparative study of ethanol production by Saccharomyces cerevisiae cells in traditional and in-situ immobilization . To study the feasibility of ethanol fermentation which in-situ and controllable immobilization can scale up and industrialize , then using 100 and 200 g/L glucose as feedstock in 2.0L bubble column .The results as shown, in 100 g/L glucose , the productivity of in-situ and controllable immobilization more than traditional immobilization by 1.35 times. In 200 g/L glucose, the ethanol concentration and productivity in the matrix is remain stable, and in-situ and controllable immobilization still up to 1.28 and 1.15 times than traditional immobilization. In this condition, the viability of in-situ immobilization almost maintain about 95% , but the viability of traditional immobilization would decrease progressively to 93% .
