Analysis of Collagen and Glucose Modulated Cell Growth within Tissue Engineered Scaffolds

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题名:	Analysis of Collagen and Glucose Modulated Cell Growth within Tissue Engineered Scaffolds
作者:	Chung,CA;Ho,SY
贡献者:	生物醫學工程研究所
关键词:	MATRIX INTERACTIONS;MATHEMATICAL-MODEL;POLYMER SCAFFOLD;CARTILAGE;PROLIFERATION;PERFUSION;CHONDROCYTES;FIBROBLASTS;BIOREACTORS;INHIBITION
日期:	2010
上传时间:	2012-03-27 16:24:57 (UTC+8)
出版者:	國立中央大學
摘要:	The strategy of tissue engineering includes seeding cells onto porous scaffolds. The cellular construct is cultured in vitro for a period of time before transplantation for the patient. Because of the intrinsic complexity of biological systems, it is valuable to have models of simulation that can assess the culture conditions and optimize experiments. This work presents a mathematical model to account for the effects of glucose and type II collagen on chondrocyte growth under static culture conditions. Dependence of cell growth on collagen was assumed as a biphasic function of collagen quantity, whereby the cell growth rate increases and then decreases with increasing collagen content. Results from simulation were compared with experimental data in literature. The model was then applied to investigate the effects of cell seeding area, demonstrating the spatiotemporal evolution of cell distribution in scaffolds. Results show that the conventional uniform seeding method may not be a good way of gaining uniform and large cell number densities at the final stage of cultivation. A seeding mode that has cells reside initially in the middle area of scaffold was shown to be able to not only reduce the diffusion limitation of nutrients but also weaken the inhibiting impact of aggregated collagen on cell growth. Therefore the middle seeding mode may result in better cell amounts and uniformities for developing tissue engineered constructs.
關聯:	ANNALS OF BIOMEDICAL ENGINEERING
显示于类别:	[生物醫學工程研究所 ] 期刊論文

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