Investigating the effects of sodium dodecyl sulfate on the aggregative behavior of hen egg-white lysozyme at acidic pH

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Title:	Investigating the effects of sodium dodecyl sulfate on the aggregative behavior of hen egg-white lysozyme at acidic pH
Authors:	Hung,YT;Lin,MS;Chen,WY;Wang,SSS
Contributors:	化學工程與材料工程學系
Keywords:	BETA-AMYLOID PEPTIDE;ISOTHERMAL TITRATION CALORIMETRY;HYDROPHOBIC FLUORESCENT-PROBE;ALZHEIMERS-DISEASE;FIBRIL FORMATION;PROTEIN-BINDING;CATIONIC GEMINI;ALPHA-SYNUCLEIN;CONGO RED;IN-VITRO
Date:	2010
Issue Date:	2012-03-27 16:25:21 (UTC+8)
Publisher:	國立中央大學
Abstract:	The research presented here is aimed at examining the effects of sodium dodecyl sulfate on the aggregarive behavior of hen egg-white lysozyme at pH 2.0. Through various spectroscopic techniques, dynamic light scattering, and electron microscopy, we first demonstrated that SDS exhibited a biphasic effect on lysozyme fibrillation. The presence of SDS at higher concentrations (e.g., 0.25, 5.00, or 20.00 mM SDS) was found to suppress fibril formation of lysozyme whereas fibrillogenic lysozyme-SDS ensemble containing beta-sheet-rich conformation was observed upon the addition of lower concentrations of SDS (e.g., 0.00, 0.06, or 0.1 mM SDS). Next, our equilibrium urea-unfolding data revealed that lysozyme samples with higher SDS concentrations showed superior thermodynamic stabilities over the ones with no or lower levels of SDS. Finally, the correlation between SOS concentration and lysozyme aggregative/fibrillogenic propensity and the underlying interacting mechanism were further explored using surface tensiometry and isothermal titration calorimetry. We believe the outcome from this work may not only help decipher the molecular mechanism of amyloid fibrillation, but also shed light on a rational design of potential therapeutic strategies for amyloid pathology. (C) 2010 Elsevier B.V. All rights reserved.
Relation:	COLLOIDS AND SURFACES B-BIOINTERFACES
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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