Measurements of Movement and Diffusion Coefficients of Single Cells on Polymeric Surface from Image Analysis

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Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/49886

Title:	Measurements of Movement and Diffusion Coefficients of Single Cells on Polymeric Surface from Image Analysis
Authors:	Higuchi,A;Ho,LY;Huang,LY;Chen,H;Chen,YJ;Ling,QD
Contributors:	化學工程與材料工程學系
Date:	2010
Issue Date:	2012-03-27 16:25:31 (UTC+8)
Publisher:	國立中央大學
Abstract:	Time-lapse digital images were taken every 30 s of PC12 cells cultured on polystyrene dishes, collagen-coated dishes and poly(L-lysine)-coated dishes in high-serum medium, low-serum medium and neurite outgrowth factor (NGF)-containing medium to investigate their diffusion coefficients (i.e., self-diffusion coefficients), D and specific movement (i.e., specific frequent movement) using image analysis and Fast Fourier Transform (FFT) analysis. D for these cells was found to fluctuate as a function of time, D varying between 0 and 0.08 mu m(2)/s. The trend observed upon examination of average D values was: D in high-serum medium >= D in low-serum medium >= D in NGF-containing medium. Image analysis and FFT analysis of single cells cultured on polymeric dishes in these three media did not have any specific frequency of cell movement between 0 and 0.0167 Hz. The high diffusion coefficient and high amplitude of power spectra of PC12 cells in high-serum medium might be attributed to the high energy necessary for their continual suppression of the mitogen-activated protein kinase (MAPK) cascade and for them to maintain their undifferentiated state. (C) Koninklijke Brill NV, Leiden, 2010
Relation:	JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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