Peroxidase Activity of DNA Aptamer-Pt Complexes Prepared with Cisplatin

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Title:	Peroxidase Activity of DNA Aptamer-Pt Complexes Prepared with Cisplatin
Authors:	Higuchi,A;Yang,ST;Siao,YD;Hsieh,PV;Fukushima,H;Chang,Y;Chen,WY
Contributors:	化學工程與材料工程學系
Date:	2010
Issue Date:	2012-03-27 16:25:42 (UTC+8)
Publisher:	國立中央大學
Abstract:	DNA aptamers carrying Pt nanoparticles prepared with cisplatin showed peroxidase enzymatic activity while retaining the specific binding ability of the aptamers. Optimal preparation conditions of DNA-Pt complex prepared with cisplatin were investigated on the synthesis at pH 7-11, a reaction time of 1-18 h and 90 degrees C. The enzymatic reaction of DNA-Pt complex obeyed Michaelis-Menten kinetics. K(M) for the DNA-Pt complex was found to be of the same order as K(M) for hemin and hemin-DNA complex, but one order of magnitude higher than that of horseradish peroxidase. A sandwich type of DNA enzyme-linked aptamer assay (DLAA) using DNA-Pt complex successively detected target protein of thrombin. DLAA using DNA-Pt complex fractioned by ultrafiltration membranes having a molecular weight cut-off of 30 000 and 300 000 showed 1.9-times higher sensitivity than DLAA using DNA-Pt complex without fraction. The DNA-Pt complex having specific size was effective for the sensitive detection of thrombin in DLAA. (c) Koninklijke Brill NV, Leiden, 2010
Relation:	JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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