Interfacing microfluidics to LDI-MS by automatic robotic spotting

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题名:	Interfacing microfluidics to LDI-MS by automatic robotic spotting
作者:	Tsao,CW;Tao,S;Chen,CF;Liu,JK;DeVoe,DL
贡献者:	機械工程學系
关键词:	DESORPTION/IONIZATION MASS-SPECTROMETRY;MALDI-TOF MS;ELECTROSPRAY-IONIZATION;SAMPLE PREPARATION;CHIP;MICROCHIP;ELECTROPHORESIS;NANOELECTROSPRAY;SENSITIVITY;PEPTIDES
日期:	2010
上传时间:	2012-03-27 17:03:33 (UTC+8)
出版者:	國立中央大學
摘要:	We developed a method of interfacing microfluidics with mass spectrometry (MS) using a robotic spotting system to automate the contact spotting process. We demonstrate that direct and automated spotting of analyte from multichannel microfluidic chips to a custom microstructured MALDI target plate was a simple, robust, and high-throughput method for interfacing parallel microchannels using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Using thermoplastic cyclic olefin copolymer (COC) polymer microfluidic chips containing eight parallel 100 mu m x 46 mu m microchannels connected to a single input port, spotting volume repeatability and MALDI-MS signal uniformity are evaluated for a panel of sample peptides. The COC microfluidic chips were fabricated by hot embossing and solvent bonding techniques followed by chip dicing to create open ends for MS interfacing. Using the automatic robotic spotting approach, microfluidic chip-based reversed-phase liquid chromatography (RPLC) separations were interfaced with electrochemically etched nanofilament silicon (nSi) target substrate, demonstrating the potential of this approach toward chip-based microfluidic separation coupled with matrix-free laser desorption/ionization mass spectrometry.
關聯:	MICROFLUIDICS AND NANOFLUIDICS
显示于类别:	[機械工程學系] 期刊論文

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