Experimental and numerical determination of cellular traction force on polymeric hydrogels

NCU Institutional Repository > 工學院 > 機械工程學系 > 期刊論文 > Item 987654321/50228

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/50228

题名:	Experimental and numerical determination of cellular traction force on polymeric hydrogels
作者:	Ng,SS;Li,C;Chan,V
贡献者:	機械工程學系
关键词:	DIGITAL-IMAGE-CORRELATION;FOCAL ADHESION KINASE;RUBBER-LIKE MATERIALS;SUBSTRATE STIFFNESS;DIRECTIONAL CONTROL;STRAIN-ENERGY;MOONEY-RIVLIN;LIVING CELLS;RHO FAMILY;MICROSCOPY
日期:	2011
上传时间:	2012-03-27 17:07:03 (UTC+8)
出版者:	國立中央大學
摘要:	Anchorage-dependent cells such as smooth muscle cells (SMCs) rely on the transmission of actomyosin-generated traction forces to adhere and migrate on the extracellular matrix. The cellular traction forces exerted by SMCs on substrate can be measured from the deformation of substrate with embedded fluorescent markers. With the synchronous use of phase-contrast and fluorescent microscopy, the deformation of polyacrylamide (PAM) gel substrate can be quantitatively determined using particle image velocimetry. This displacement map is then input as boundary conditions for the stress analysis on PAM gel by the finite-element method. In addition to optical microscopy, atomic force microscopy was also used to characterize the PAM substrate using the contact mode, from which the elasticity of PAM can be quantified using Hertzian theory. This provides baseline information for the stress analysis of PAM gel deformation. The material model introduced for the computational part is the Mooney-Rivlin constitutive law because of its long proven usefulness in predicting polymers' mechanical behaviour. Numerical results showed that adhesive stresses are high around the cell edges, which is in accordance with the general phenomena of cellular focal adhesion. Further calculations on the total traction forces indicate a slightly contact-dominated regime for a broad range of Mooney-Rivlin stiffnesses.
關聯:	INTERFACE FOCUS
显示于类别:	[機械工程學系] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	532	检视/开启

在NCUIR中所有的数据项都受到原著作权保护.

社群 sharing

数据加载中.....