A 9 bp cis-element in the promoters of class I small heat shock protein genes on chromosome 3 in rice mediates L-azetidine-2-carboxylic acid and heat shock responses

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題名:	A 9 bp cis-element in the promoters of class I small heat shock protein genes on chromosome 3 in rice mediates L-azetidine-2-carboxylic acid and heat shock responses
作者:	Guan,JC;Yeh,CH;Lin,YP;Ke,YT;Chen,MT;You,JW;Liu,YH;Lu,CA;Wu,SJ;Lin,CY
貢獻者:	生命科學系
關鍵詞:	STRESS TRANSCRIPTION FACTORS;SOYBEAN SEEDLINGS;DEVELOPMENTAL REGULATION;REGULATORY ELEMENTS;UPSTREAM SEQUENCES;EXPRESSION;ARABIDOPSIS;ACCUMULATION;FACTOR-1;BINDING
日期:	2010
上傳時間:	2012-03-27 18:08:27 (UTC+8)
出版者:	國立中央大學
摘要:	In rice, the class I small heat shock protein (sHSP-CI) genes were found to be selectively induced by L-azetidine-2-carboxylic acid (AZC) on chromosome 3 but not chromosome 1. Here it is shown that a novel cis-responsive element contributed to the differential regulation. By serial deletion and computational analysis, a 9 bp putative AZC-responsive element (AZRE), GTCCTGGAC, located between nucleotides -186 and -178 relative to the transcription initiation site of Oshsp17.3 was revealed. Deletion of this putative AZRE from the promoter abolished its ability to be induced by AZC. Moreover, electrophoretic mobility shift assay (EMSA) revealed that the AZRE interacted specifically with nuclear proteins from AZC-treated rice seedlings. Two AZRE-protein complexes were detected by EMSA, one of which could be competed out by a canonical heat shock element (HSE). Deletion of the AZRE also affected the HS response. Furthermore, transient co-expression of the heat shock factor OsHsfA4b with the AZRE in the promoter of Oshsp17.3 was effective. The requirement for the putative AZRE for AZC and HS responses in transgenic Arabidopsis was also shown. Thus, AZRE represents an alternative form of heat HSE, and its interaction with canonical HSEs through heat shock factors may be required to respond to HS and AZC.
關聯:	JOURNAL OF EXPERIMENTAL BOTANY
顯示於類別:	[生命科學系] 期刊論文

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