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    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/50723


    Title: A tryptophan-rich peptide acts as a transcription activation domain
    Authors: Lin,CH;Lin,G;Chang,CP;Wang,CC
    Contributors: 生命科學系
    Keywords: TRANSFER-RNA SYNTHETASE;METHIONYL-TRANSFER-RNA;BINDING DOMAIN;SACCHAROMYCES-CEREVISIAE;GENE ENCODES;MITOCHONDRIAL;TRANSLATION;COFACTOR;COMPLEX;TARGET
    Date: 2010
    Issue Date: 2012-03-27 18:08:45 (UTC+8)
    Publisher: 國立中央大學
    Abstract: Background: Eukaryotic transcription activators normally consist of a sequence-specific DNA-binding domain (DBD) and a transcription activation domain (AD). While many sequence patterns and motifs have been defined for DBDs, ADs do not share easily recognizable motifs or structures. Results: We report herein that the N-terminal domain of yeast valyl-tRNA synthetase can function as an AD when fused to a DNA-binding protein, LexA, and turn on reporter genes with distinct LexA-responsive promoters. The transcriptional activity was mainly attributed to a five-residue peptide, WYDWW, near the C-terminus of the N domain. Remarkably, the pentapeptide per se retained much of the transcriptional activity. Mutations which substituted tryptophan residues for both of the non-tryptophan residues in the pentapeptide (resulting in W(5)) significantly enhanced its activity (similar to 1.8-fold), while mutations which substituted aromatic residues with alanine residues severely impaired its activity. Accordingly, a much more active peptide, pentatryptophan (W(7)), was produced, which elicited similar to 3-fold higher activity than that of the native pentapeptide and the N domain. Further study indicated that W(7) mediates transcription activation through interacting with the general transcription factor, TFIIB. Conclusions: Since W(7) shares no sequence homology or features with any known transcription activators, it may represent a novel class of AD.
    Relation: BMC MOLECULAR BIOLOGY
    Appears in Collections:[生命科學系] 期刊論文

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