Green Tea Catechins: Inhibitors of Glycerol-3-Phosphate Dehydrogenase

NCU Institutional Repository > 生醫理工學院 > 生命科學系 > 期刊論文 > Item 987654321/50740

請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/50740

題名:	Green Tea Catechins: Inhibitors of Glycerol-3-Phosphate Dehydrogenase
作者:	Kao,CC;Wu,BT;Tsuei,YW;Shih,LJ;Kuo,YL;Kao,YH
貢獻者:	生命科學系
關鍵詞:	3T3-L1 CELLS;EPIGALLOCATECHIN GALLATE;POLYPHENOLS;(-)-EPIGALLOCATECHIN-3-GALLATE;ACCUMULATION;MECHANISMS;CAFFEINE;GENE
日期:	2010
上傳時間:	2012-03-27 18:09:07 (UTC+8)
出版者:	國立中央大學
摘要:	Green tea catechins, especially (-)-epigallocatechin-3-gallate (EGCG), are known to regulate obesity and fat accumulation. We performed a kinetic analysis in a cell-free system to determine the mode of inhibition of glycerol-3-phosphate dehydrogenase (GPDH; EC 1.1.1.8) by EGCG. GPDH catalyzes the beta-nicotinamide adenine dinucleotide (NADH)-dependent reduction of dihydroxyacetone phosphate (DHAP) to yield glycerol-3-phosphate, which serves as one of the major precursors of triacylglycerols. We found that EGCG dose-dependently inhibited GPDH activity at a concentration of approximately 20 mu M for 50% inhibition. The IC(50) values of other green tea catechins, such as (-)-epicatechin, (-)-epicatechin-3-gallate, and (-)-epigallocatechin, were all above 100 mu M. This suggests a catechin type-dependent effect. Based on double-reciprocal plots of the kinetic data, EGCG was a noncompetitive inhibitor of the GPDH substrates, NADH and DHAP, with respective inhibition constants (Ki) of 18 and 31 mu M. Results of this study possibly support previous studies that EGCG mediates fat content.
關聯:	PLANTA MEDICA
顯示於類別:	[生命科學系] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	532	檢視/開啟

在NCUIR中所有的資料項目都受到原著作權保護.

社群 sharing

資料載入中.....